Fluoxetine Increases the Expression of miR-572 and miR-663a in Human Neuroblastoma Cell Lines.
Evidence suggests neuroprotective effects of fluoxetine, a selective serotonin reuptake inhibitor (SSRI), on the developed neurons in the adult brain. In contrast, the drug may be deleterious to immature or undifferentiated neural cells, although the mechanism is unclear. Recent investigations have...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5055328?pdf=render |
| _version_ | 1818942414483619840 |
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| author | Mahesh Mundalil Vasu Ayyappan Anitha Taro Takahashi Ismail Thanseem Keiko Iwata Tetsuya Asakawa Katsuaki Suzuki |
| author_facet | Mahesh Mundalil Vasu Ayyappan Anitha Taro Takahashi Ismail Thanseem Keiko Iwata Tetsuya Asakawa Katsuaki Suzuki |
| author_sort | Mahesh Mundalil Vasu |
| collection | DOAJ |
| description | Evidence suggests neuroprotective effects of fluoxetine, a selective serotonin reuptake inhibitor (SSRI), on the developed neurons in the adult brain. In contrast, the drug may be deleterious to immature or undifferentiated neural cells, although the mechanism is unclear. Recent investigations have suggested that microRNAs (miRNA) may be critical for effectiveness of psychotropic drugs including SSRI. We investigated whether fluoxetine could modulate expressions of neurologically relevant miRNAs in two neuroblastoma SK-N-SH and SH-SY5Y cell lines. Initial screening results revealed that three (miR-489, miR-572 and miR-663a) and four (miR-320a, miR-489, miR-572 and miR-663a) miRNAs were up-regulated in SK-N-SH cells and SH-SY5Y cells, respectively, after 24 hours treatment of fluoxetine (1-25 μM). Cell viability was reduced according to the dose of fluoxetine. The upregulation of miR-572 and miR-663a was consistent in both the SH-SY5Y and SK-N-SH cells, confirmed by a larger scale culture condition. Our data is the first in vitro evidence that fluoxetine could increase the expression of miRNAs in undifferentiated neural cells, and that putative target genes of those miRNAs have been shown to be involved in fundamental neurodevelopmental processes. |
| first_indexed | 2024-12-20T07:11:03Z |
| format | Article |
| id | doaj.art-14a8445d0c204343b7278d2dee355a93 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T07:11:03Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-14a8445d0c204343b7278d2dee355a932022-12-21T19:48:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011110e016442510.1371/journal.pone.0164425Fluoxetine Increases the Expression of miR-572 and miR-663a in Human Neuroblastoma Cell Lines.Mahesh Mundalil VasuAyyappan AnithaTaro TakahashiIsmail ThanseemKeiko IwataTetsuya AsakawaKatsuaki SuzukiEvidence suggests neuroprotective effects of fluoxetine, a selective serotonin reuptake inhibitor (SSRI), on the developed neurons in the adult brain. In contrast, the drug may be deleterious to immature or undifferentiated neural cells, although the mechanism is unclear. Recent investigations have suggested that microRNAs (miRNA) may be critical for effectiveness of psychotropic drugs including SSRI. We investigated whether fluoxetine could modulate expressions of neurologically relevant miRNAs in two neuroblastoma SK-N-SH and SH-SY5Y cell lines. Initial screening results revealed that three (miR-489, miR-572 and miR-663a) and four (miR-320a, miR-489, miR-572 and miR-663a) miRNAs were up-regulated in SK-N-SH cells and SH-SY5Y cells, respectively, after 24 hours treatment of fluoxetine (1-25 μM). Cell viability was reduced according to the dose of fluoxetine. The upregulation of miR-572 and miR-663a was consistent in both the SH-SY5Y and SK-N-SH cells, confirmed by a larger scale culture condition. Our data is the first in vitro evidence that fluoxetine could increase the expression of miRNAs in undifferentiated neural cells, and that putative target genes of those miRNAs have been shown to be involved in fundamental neurodevelopmental processes.http://europepmc.org/articles/PMC5055328?pdf=render |
| spellingShingle | Mahesh Mundalil Vasu Ayyappan Anitha Taro Takahashi Ismail Thanseem Keiko Iwata Tetsuya Asakawa Katsuaki Suzuki Fluoxetine Increases the Expression of miR-572 and miR-663a in Human Neuroblastoma Cell Lines. PLoS ONE |
| title | Fluoxetine Increases the Expression of miR-572 and miR-663a in Human Neuroblastoma Cell Lines. |
| title_full | Fluoxetine Increases the Expression of miR-572 and miR-663a in Human Neuroblastoma Cell Lines. |
| title_fullStr | Fluoxetine Increases the Expression of miR-572 and miR-663a in Human Neuroblastoma Cell Lines. |
| title_full_unstemmed | Fluoxetine Increases the Expression of miR-572 and miR-663a in Human Neuroblastoma Cell Lines. |
| title_short | Fluoxetine Increases the Expression of miR-572 and miR-663a in Human Neuroblastoma Cell Lines. |
| title_sort | fluoxetine increases the expression of mir 572 and mir 663a in human neuroblastoma cell lines |
| url | http://europepmc.org/articles/PMC5055328?pdf=render |
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