Protective Action of Phosphorus 6CH in SARS-CoV-2 Spike Protein Induced Pathogenecity in Gallus-gallus Embryo
Introduction: In the search of effective medicines against Coronavirus Disease-2019 (COVID-19) besides the conventional mode of treatment many medicines belonging to alternative therapeutics claimed to be effective in this disease. In homeopathy- a branch of alternative medicine, some medicines are...
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JCDR Research and Publications Private Limited
2022-08-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://www.jcdr.net/articles/PDF/16760/56788_CE(AD)_F[SK]_PF1(AG_SH)_PFA(AG_KM)_PN(KM).pdf |
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| author | Debasmita Chatterjee Krishnendu Paira Pritam Goswami Sayak Ghosh Dharitri Choudhuri Satadal Das |
| author_facet | Debasmita Chatterjee Krishnendu Paira Pritam Goswami Sayak Ghosh Dharitri Choudhuri Satadal Das |
| author_sort | Debasmita Chatterjee |
| collection | DOAJ |
| description | Introduction: In the search of effective medicines against Coronavirus Disease-2019 (COVID-19) besides the conventional mode of treatment many medicines belonging to alternative therapeutics claimed to be effective in this disease. In homeopathy- a branch of alternative medicine, some medicines are claimed to be effective in COVID-19 after human trials.
Aim: To study whether ultradiluted preparation of Phosphorus 6CH (centesimal (C) dilutions, using Hanhemann’s (H) dilution method) can protect damaging action of Delta Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) spike protein Receptor Binding Domain (RBD) in Gallus gallus embryo in relation to their gross appearances, histopathological changes and cytokine changes.
Materials and Methods: The present in-vivo study was an experimental analysis carried out at the Genetic Research Laboratory of Heritage Institute of Technology, Kolkata, West Bengal, India. The whole experimental study was on 20,000 subjects in a time period of November 2021 to January 2022 and the data collected were analysed using statistical software Minitab. About 14 days old Gallus gallus embryonated eggs were inoculated with the antigen along with the vehicle alcohol controls. The Phosphorus 6CH was used to see whether it can prevent or cure the damaging action of the spike protein in the embryo in different experimental sets.
Results: The notable finding in this experiment is the remarkable elevated expression of Interleukin (IL)-10 gene in the curative, preventive sets as well as in the medicine control sets in comparison to antigen and alcohol control sets. In case of Transforming Growth Factor, (TGF) β1 there was enhanced expression of TGF β1 gene in the alcohol 6C set and antigen set which gets ameliorated with Phosphorus 6CH. The morbid anatomy of the embryo and the histopathological picture of the liver of the embryo also reflected similar findings in these two experimental sets. After statistical analysis it was found that there was significant correlation in between Interferon (IF) γ and IL-10 in these experimental results which appears very important.
Conclusion: The homeopathic medicine phosphorus 6CH is capable of maintaining cytokine balance in Delta SARS-CoV-2 spike protein RBD induced pathogenecity in Gallus gallus embryo. |
| first_indexed | 2024-04-10T09:32:45Z |
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| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-04-10T09:32:45Z |
| publishDate | 2022-08-01 |
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| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-14a86baafcb54ee29ea4e202468433ef2023-02-18T09:58:37ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2022-08-01168DC53DC5710.7860/JCDR/2022/56788.16760Protective Action of Phosphorus 6CH in SARS-CoV-2 Spike Protein Induced Pathogenecity in Gallus-gallus EmbryoDebasmita Chatterjee0Krishnendu Paira1Pritam Goswami2Sayak Ghosh3Dharitri Choudhuri4Satadal Das5Research Associate, Department of Genetic Research Laboratory, Heritage Institute of Technology, Kolkata, West Bengal, India.Chief Coordinator-Research Projects, Department of Genetic Research Laboratory, Heritage Institute of Technology, Kolkata, West Bengal, India.MD-PGT, Department of Repertory, West Bengal University of Health Sciences, Kolkata, West Bengal, India.Medical Officer, Department of Medical OPD, Kelomal PHC, Midnapur, West Bengal, India.Postgraduate Student, Department of Biotechnology, St. Xaviers’ College, Kolkata, West Bengal, India.Principal Scientist, Department of Genetic Research Laboratory, Heritage Institute of Technology, Kolkata, West Bengal, India.Introduction: In the search of effective medicines against Coronavirus Disease-2019 (COVID-19) besides the conventional mode of treatment many medicines belonging to alternative therapeutics claimed to be effective in this disease. In homeopathy- a branch of alternative medicine, some medicines are claimed to be effective in COVID-19 after human trials. Aim: To study whether ultradiluted preparation of Phosphorus 6CH (centesimal (C) dilutions, using Hanhemann’s (H) dilution method) can protect damaging action of Delta Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) spike protein Receptor Binding Domain (RBD) in Gallus gallus embryo in relation to their gross appearances, histopathological changes and cytokine changes. Materials and Methods: The present in-vivo study was an experimental analysis carried out at the Genetic Research Laboratory of Heritage Institute of Technology, Kolkata, West Bengal, India. The whole experimental study was on 20,000 subjects in a time period of November 2021 to January 2022 and the data collected were analysed using statistical software Minitab. About 14 days old Gallus gallus embryonated eggs were inoculated with the antigen along with the vehicle alcohol controls. The Phosphorus 6CH was used to see whether it can prevent or cure the damaging action of the spike protein in the embryo in different experimental sets. Results: The notable finding in this experiment is the remarkable elevated expression of Interleukin (IL)-10 gene in the curative, preventive sets as well as in the medicine control sets in comparison to antigen and alcohol control sets. In case of Transforming Growth Factor, (TGF) β1 there was enhanced expression of TGF β1 gene in the alcohol 6C set and antigen set which gets ameliorated with Phosphorus 6CH. The morbid anatomy of the embryo and the histopathological picture of the liver of the embryo also reflected similar findings in these two experimental sets. After statistical analysis it was found that there was significant correlation in between Interferon (IF) γ and IL-10 in these experimental results which appears very important. Conclusion: The homeopathic medicine phosphorus 6CH is capable of maintaining cytokine balance in Delta SARS-CoV-2 spike protein RBD induced pathogenecity in Gallus gallus embryo.https://www.jcdr.net/articles/PDF/16760/56788_CE(AD)_F[SK]_PF1(AG_SH)_PFA(AG_KM)_PN(KM).pdfcytokineshistopathologyultradilution |
| spellingShingle | Debasmita Chatterjee Krishnendu Paira Pritam Goswami Sayak Ghosh Dharitri Choudhuri Satadal Das Protective Action of Phosphorus 6CH in SARS-CoV-2 Spike Protein Induced Pathogenecity in Gallus-gallus Embryo Journal of Clinical and Diagnostic Research cytokines histopathology ultradilution |
| title | Protective Action of Phosphorus 6CH in SARS-CoV-2 Spike Protein Induced Pathogenecity in Gallus-gallus Embryo |
| title_full | Protective Action of Phosphorus 6CH in SARS-CoV-2 Spike Protein Induced Pathogenecity in Gallus-gallus Embryo |
| title_fullStr | Protective Action of Phosphorus 6CH in SARS-CoV-2 Spike Protein Induced Pathogenecity in Gallus-gallus Embryo |
| title_full_unstemmed | Protective Action of Phosphorus 6CH in SARS-CoV-2 Spike Protein Induced Pathogenecity in Gallus-gallus Embryo |
| title_short | Protective Action of Phosphorus 6CH in SARS-CoV-2 Spike Protein Induced Pathogenecity in Gallus-gallus Embryo |
| title_sort | protective action of phosphorus 6ch in sars cov 2 spike protein induced pathogenecity in gallus gallus embryo |
| topic | cytokines histopathology ultradilution |
| url | https://www.jcdr.net/articles/PDF/16760/56788_CE(AD)_F[SK]_PF1(AG_SH)_PFA(AG_KM)_PN(KM).pdf |
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