The best time of cytotoxicity for extracted cell wall from Lactobacillus casei and paracasei in K562 cell line
Background: The aim of this study was to evaluate the effect of extracted cell walls from Lactobacillus casei and Lactobacillus paracasei as probiotic bacteria (isolated from common carp intestine) on K562 and the role of cell concentration on the results of MTT [3-(4,5-Dimethylthiazol-2-yl)2,5- Dip...
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| Format: | Article |
| Language: | fas |
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Tehran University of Medical Sciences
2013-02-01
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| Series: | Tehran University Medical Journal |
| Subjects: | |
| Online Access: | http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-53&slc_lang=en&sid=1 |
| _version_ | 1818303292797616128 |
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| author | Riki M Farokhi F Tukmechi A |
| author_facet | Riki M Farokhi F Tukmechi A |
| author_sort | Riki M |
| collection | DOAJ |
| description | Background: The aim of this study was to evaluate the effect of extracted cell walls from Lactobacillus casei and Lactobacillus paracasei as probiotic bacteria (isolated from common carp intestine) on K562 and the role of cell concentration on the results of MTT [3-(4,5-Dimethylthiazol-2-yl)2,5- Diphenyl tetrazolium Bromide] test.Methods: For this purpose, bacteria were cultured in specific medium (MRS broth) at anaerobic condition for 24-48 hour. After incubation period culture medium was centri-fuged, then the cells were washed twice with PBS buffer to remove additional medium. Finally, collected bacterial cell disrupted by Sonication and cell walls were separated from other components by centrifugation. After that, different concentrations of cell walls (500, 1000, 2000 and 4000 µg/ml) were prepared in RPMI medium for each bacteria, separately. Then anticancer properties of the cell walls were determined in vitro at 12, 24, 48 and 72 h, also the effect of K562 concentration was assayed with MTT technique.Results: The results showed extracted cell wall from both probiotic statistically (P=0.098) have anti turmeric properties in K562 and their properties will arise in relation with concentration. As well as, we found that the number of cell had not any affect on the result of MTT assay.Conclusion: We conclude that the cytotoxicity property of extracted cell wall is related in the type of bacteria, but this anticancer property would warrant further study on the clinical application of extracted cell wall. |
| first_indexed | 2024-12-13T05:52:29Z |
| format | Article |
| id | doaj.art-14a8b23299a54e0bb8bf2676d53de975 |
| institution | Directory Open Access Journal |
| issn | 1683-1764 1735-7322 |
| language | fas |
| last_indexed | 2024-12-13T05:52:29Z |
| publishDate | 2013-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Tehran University Medical Journal |
| spelling | doaj.art-14a8b23299a54e0bb8bf2676d53de9752022-12-21T23:57:30ZfasTehran University of Medical SciencesTehran University Medical Journal1683-17641735-73222013-02-017011691699The best time of cytotoxicity for extracted cell wall from Lactobacillus casei and paracasei in K562 cell lineRiki MFarokhi FTukmechi ABackground: The aim of this study was to evaluate the effect of extracted cell walls from Lactobacillus casei and Lactobacillus paracasei as probiotic bacteria (isolated from common carp intestine) on K562 and the role of cell concentration on the results of MTT [3-(4,5-Dimethylthiazol-2-yl)2,5- Diphenyl tetrazolium Bromide] test.Methods: For this purpose, bacteria were cultured in specific medium (MRS broth) at anaerobic condition for 24-48 hour. After incubation period culture medium was centri-fuged, then the cells were washed twice with PBS buffer to remove additional medium. Finally, collected bacterial cell disrupted by Sonication and cell walls were separated from other components by centrifugation. After that, different concentrations of cell walls (500, 1000, 2000 and 4000 µg/ml) were prepared in RPMI medium for each bacteria, separately. Then anticancer properties of the cell walls were determined in vitro at 12, 24, 48 and 72 h, also the effect of K562 concentration was assayed with MTT technique.Results: The results showed extracted cell wall from both probiotic statistically (P=0.098) have anti turmeric properties in K562 and their properties will arise in relation with concentration. As well as, we found that the number of cell had not any affect on the result of MTT assay.Conclusion: We conclude that the cytotoxicity property of extracted cell wall is related in the type of bacteria, but this anticancer property would warrant further study on the clinical application of extracted cell wall.http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-53&slc_lang=en&sid=1anticancer propertiesK562MTT testprobiotic |
| spellingShingle | Riki M Farokhi F Tukmechi A The best time of cytotoxicity for extracted cell wall from Lactobacillus casei and paracasei in K562 cell line Tehran University Medical Journal anticancer properties K562 MTT test probiotic |
| title | The best time of cytotoxicity for extracted cell wall from Lactobacillus casei and paracasei in K562 cell line |
| title_full | The best time of cytotoxicity for extracted cell wall from Lactobacillus casei and paracasei in K562 cell line |
| title_fullStr | The best time of cytotoxicity for extracted cell wall from Lactobacillus casei and paracasei in K562 cell line |
| title_full_unstemmed | The best time of cytotoxicity for extracted cell wall from Lactobacillus casei and paracasei in K562 cell line |
| title_short | The best time of cytotoxicity for extracted cell wall from Lactobacillus casei and paracasei in K562 cell line |
| title_sort | best time of cytotoxicity for extracted cell wall from lactobacillus casei and paracasei in k562 cell line |
| topic | anticancer properties K562 MTT test probiotic |
| url | http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-53&slc_lang=en&sid=1 |
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