Biocomposite Hydrogels for the Treatment of Bacterial Infections: Physicochemical Characterization and In Vitro Assessment
Hydrogels based on natural and synthetic polymers and inorganic nanoparticles proved to be a viable strategy in the fight against some Gram-positive and Gram-negative bacteria. Additionally, numerous studies have demonstrated the advantages of using ZnO nanoparticles in medicine due to their high an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/12/2079 |
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| author | Delia Mihaela Rata Anca Niculina Cadinoiu Marcel Popa Leonard Ionut Atanase Oana Maria Daraba Irina Popescu Laura Ecaterina Romila Daniela Luminita Ichim |
| author_facet | Delia Mihaela Rata Anca Niculina Cadinoiu Marcel Popa Leonard Ionut Atanase Oana Maria Daraba Irina Popescu Laura Ecaterina Romila Daniela Luminita Ichim |
| author_sort | Delia Mihaela Rata |
| collection | DOAJ |
| description | Hydrogels based on natural and synthetic polymers and inorganic nanoparticles proved to be a viable strategy in the fight against some Gram-positive and Gram-negative bacteria. Additionally, numerous studies have demonstrated the advantages of using ZnO nanoparticles in medicine due to their high antibacterial efficacy and relatively low cost. Consequently, the purpose of our study was to incorporate ZnO nanoparticles into chitosan/poly (vinyl alcohol)-based hydrogels in order to obtain a biocomposite with antimicrobial properties. These biocomposite hydrogels, prepared by a double crosslinking (covalent and ionic) were characterized from a structural, morphological, swelling degree, and mechanical point of view. FTIR spectroscopy demonstrated both the apparition of new imine and acetal bonds due to covalent crosslinking and the presence of the sulfate group following ionic crosslinking. The morphology, swelling degree, and mechanical properties of the obtained hydrogels were influenced by both the degree of covalent crosslinking and the amount of ZnO nanoparticles incorporated. In vitro cytotoxicity assessment showed that hydrogels without ZnONPs are non-cytotoxic while the biocomposite hydrogels are weak (with 3% ZnONPs) or moderately (with 4 and 5% ZnONPs) cytotoxic. Compared to nanoparticle-free hydrogels, the biocomposite hydrogels show significant antimicrobial activity against <i>S. aureus</i>, <i>E. coli</i>, and <i>K. pneumonia</i>. |
| first_indexed | 2024-03-10T03:19:08Z |
| format | Article |
| id | doaj.art-14a937b5681e4be2a66f73f4ef734f48 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T03:19:08Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-14a937b5681e4be2a66f73f4ef734f482023-11-23T10:05:37ZengMDPI AGPharmaceutics1999-49232021-12-011312207910.3390/pharmaceutics13122079Biocomposite Hydrogels for the Treatment of Bacterial Infections: Physicochemical Characterization and In Vitro AssessmentDelia Mihaela Rata0Anca Niculina Cadinoiu1Marcel Popa2Leonard Ionut Atanase3Oana Maria Daraba4Irina Popescu5Laura Ecaterina Romila6Daniela Luminita Ichim7Faculty of Medical Dentistry, Apollonia University of Iasi, 700511 Iasi, RomaniaFaculty of Medical Dentistry, Apollonia University of Iasi, 700511 Iasi, RomaniaFaculty of Medical Dentistry, Apollonia University of Iasi, 700511 Iasi, RomaniaFaculty of Medical Dentistry, Apollonia University of Iasi, 700511 Iasi, RomaniaFaculty of Medical Dentistry, Apollonia University of Iasi, 700511 Iasi, RomaniaPetru Poni Institute of Macromolecular Chemistry, Aleea Grigore Ghica Voda 41A, 700487 Iasi, RomaniaFaculty of Medical Dentistry, Apollonia University of Iasi, 700511 Iasi, RomaniaFaculty of Medical Dentistry, Apollonia University of Iasi, 700511 Iasi, RomaniaHydrogels based on natural and synthetic polymers and inorganic nanoparticles proved to be a viable strategy in the fight against some Gram-positive and Gram-negative bacteria. Additionally, numerous studies have demonstrated the advantages of using ZnO nanoparticles in medicine due to their high antibacterial efficacy and relatively low cost. Consequently, the purpose of our study was to incorporate ZnO nanoparticles into chitosan/poly (vinyl alcohol)-based hydrogels in order to obtain a biocomposite with antimicrobial properties. These biocomposite hydrogels, prepared by a double crosslinking (covalent and ionic) were characterized from a structural, morphological, swelling degree, and mechanical point of view. FTIR spectroscopy demonstrated both the apparition of new imine and acetal bonds due to covalent crosslinking and the presence of the sulfate group following ionic crosslinking. The morphology, swelling degree, and mechanical properties of the obtained hydrogels were influenced by both the degree of covalent crosslinking and the amount of ZnO nanoparticles incorporated. In vitro cytotoxicity assessment showed that hydrogels without ZnONPs are non-cytotoxic while the biocomposite hydrogels are weak (with 3% ZnONPs) or moderately (with 4 and 5% ZnONPs) cytotoxic. Compared to nanoparticle-free hydrogels, the biocomposite hydrogels show significant antimicrobial activity against <i>S. aureus</i>, <i>E. coli</i>, and <i>K. pneumonia</i>.https://www.mdpi.com/1999-4923/13/12/2079chitosanpoly (vinyl alcohol)ZnO nanoparticleshydrogelsantimicrobial activities |
| spellingShingle | Delia Mihaela Rata Anca Niculina Cadinoiu Marcel Popa Leonard Ionut Atanase Oana Maria Daraba Irina Popescu Laura Ecaterina Romila Daniela Luminita Ichim Biocomposite Hydrogels for the Treatment of Bacterial Infections: Physicochemical Characterization and In Vitro Assessment Pharmaceutics chitosan poly (vinyl alcohol) ZnO nanoparticles hydrogels antimicrobial activities |
| title | Biocomposite Hydrogels for the Treatment of Bacterial Infections: Physicochemical Characterization and In Vitro Assessment |
| title_full | Biocomposite Hydrogels for the Treatment of Bacterial Infections: Physicochemical Characterization and In Vitro Assessment |
| title_fullStr | Biocomposite Hydrogels for the Treatment of Bacterial Infections: Physicochemical Characterization and In Vitro Assessment |
| title_full_unstemmed | Biocomposite Hydrogels for the Treatment of Bacterial Infections: Physicochemical Characterization and In Vitro Assessment |
| title_short | Biocomposite Hydrogels for the Treatment of Bacterial Infections: Physicochemical Characterization and In Vitro Assessment |
| title_sort | biocomposite hydrogels for the treatment of bacterial infections physicochemical characterization and in vitro assessment |
| topic | chitosan poly (vinyl alcohol) ZnO nanoparticles hydrogels antimicrobial activities |
| url | https://www.mdpi.com/1999-4923/13/12/2079 |
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