Phenolic Content, Antioxidant Effects and Tyrosinase Inhibitory Activity of Extract of Some Stachys Species from Iran
Background: Given the importance of Stachys genus in Iran and their significant biological activity, we collected three species of this plant: St. aucheri Benth., Stachys benthamiana Boiss and St. inflata Benth. from different regions of Iran. We examined the free radical scavenging potential, total...
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| Format: | Article |
| Language: | English |
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Bushehr University of Medical Sciences
2019-10-01
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| Series: | Iranian South Medical Journal |
| Subjects: | |
| Online Access: | http://ismj.bpums.ac.ir/article-1-1142-en.html |
| _version_ | 1818230000203071488 |
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| author | Najmeh Edraki Mojtaba Asadollahi Hajar Hemmatian Mehdi Khoshneviszadeh Omidreza Firuzi Amirhossein Sakhteman Amireza Jasbi |
| author_facet | Najmeh Edraki Mojtaba Asadollahi Hajar Hemmatian Mehdi Khoshneviszadeh Omidreza Firuzi Amirhossein Sakhteman Amireza Jasbi |
| author_sort | Najmeh Edraki |
| collection | DOAJ |
| description | Background: Given the importance of Stachys genus in Iran and their significant biological activity, we collected three species of this plant: St. aucheri Benth., Stachys benthamiana Boiss and St. inflata Benth. from different regions of Iran. We examined the free radical scavenging potential, total phenol content and tyrosinase inhibitory activity of their methanol, methanol 80% and dichloromethane extracts.
Materials and Methods: Antioxidant activity and total phenol content of the mentioned extracts were assessed using DPPH and FolinCiocalteu reagent, respectively. The inhibitory activity of the extracts on tyrosinase was also investigated.
Results: Total phenol content of different extracts was high in the extracts as 98.5-139.7 mg eq. gallic acid/g in methanol 80% extract, 36.8-54.7 mg eq. gallic acid/g in methanol extract and 17.9-44.9 mg eq. gallic acid/g in dichloromethane extract. Furthermore, their antioxidant activity was correlated with total phenol content of the extracts. Methanol 80% extract had the highest total phenol content and antioxidant activity. Evaluation of the tyrosinase inhibitory potential of the extracts revealed that St.inflata exhibited a considerable inhibitory potential (IC50= 5.4 ± 0.6 µM).
Conclusion: It can be concluded that tyrosinase inhibitory activity of Stachys genus might be due to the less polar compounds and these compounds are mostly present in the dichloromethane extract of St. inflatai over St. aucheri and St. benthamiana |
| first_indexed | 2024-12-12T10:27:32Z |
| format | Article |
| id | doaj.art-14a97898368b432c9cbe8c6edf8cde42 |
| institution | Directory Open Access Journal |
| issn | 1735-4374 1735-6954 |
| language | English |
| last_indexed | 2024-12-12T10:27:32Z |
| publishDate | 2019-10-01 |
| publisher | Bushehr University of Medical Sciences |
| record_format | Article |
| series | Iranian South Medical Journal |
| spelling | doaj.art-14a97898368b432c9cbe8c6edf8cde422022-12-22T00:27:25ZengBushehr University of Medical SciencesIranian South Medical Journal1735-43741735-69542019-10-01224191199Phenolic Content, Antioxidant Effects and Tyrosinase Inhibitory Activity of Extract of Some Stachys Species from IranNajmeh Edraki0Mojtaba Asadollahi1Hajar Hemmatian2Mehdi Khoshneviszadeh3Omidreza Firuzi4Amirhossein Sakhteman5Amireza Jasbi6 Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran<br>Medicinal chemistry department, School of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Medicinal chemistry department, School of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Background: Given the importance of Stachys genus in Iran and their significant biological activity, we collected three species of this plant: St. aucheri Benth., Stachys benthamiana Boiss and St. inflata Benth. from different regions of Iran. We examined the free radical scavenging potential, total phenol content and tyrosinase inhibitory activity of their methanol, methanol 80% and dichloromethane extracts. Materials and Methods: Antioxidant activity and total phenol content of the mentioned extracts were assessed using DPPH and FolinCiocalteu reagent, respectively. The inhibitory activity of the extracts on tyrosinase was also investigated. Results: Total phenol content of different extracts was high in the extracts as 98.5-139.7 mg eq. gallic acid/g in methanol 80% extract, 36.8-54.7 mg eq. gallic acid/g in methanol extract and 17.9-44.9 mg eq. gallic acid/g in dichloromethane extract. Furthermore, their antioxidant activity was correlated with total phenol content of the extracts. Methanol 80% extract had the highest total phenol content and antioxidant activity. Evaluation of the tyrosinase inhibitory potential of the extracts revealed that St.inflata exhibited a considerable inhibitory potential (IC50= 5.4 ± 0.6 µM). Conclusion: It can be concluded that tyrosinase inhibitory activity of Stachys genus might be due to the less polar compounds and these compounds are mostly present in the dichloromethane extract of St. inflatai over St. aucheri and St. benthamianahttp://ismj.bpums.ac.ir/article-1-1142-en.htmlTyrosinasetyrosinase inhibitiontotal phenolDPPHantioxidant activityStachys |
| spellingShingle | Najmeh Edraki Mojtaba Asadollahi Hajar Hemmatian Mehdi Khoshneviszadeh Omidreza Firuzi Amirhossein Sakhteman Amireza Jasbi Phenolic Content, Antioxidant Effects and Tyrosinase Inhibitory Activity of Extract of Some Stachys Species from Iran Iranian South Medical Journal Tyrosinase tyrosinase inhibition total phenol DPPH antioxidant activity Stachys |
| title | Phenolic Content, Antioxidant Effects and Tyrosinase Inhibitory Activity of Extract of Some Stachys Species from Iran |
| title_full | Phenolic Content, Antioxidant Effects and Tyrosinase Inhibitory Activity of Extract of Some Stachys Species from Iran |
| title_fullStr | Phenolic Content, Antioxidant Effects and Tyrosinase Inhibitory Activity of Extract of Some Stachys Species from Iran |
| title_full_unstemmed | Phenolic Content, Antioxidant Effects and Tyrosinase Inhibitory Activity of Extract of Some Stachys Species from Iran |
| title_short | Phenolic Content, Antioxidant Effects and Tyrosinase Inhibitory Activity of Extract of Some Stachys Species from Iran |
| title_sort | phenolic content antioxidant effects and tyrosinase inhibitory activity of extract of some stachys species from iran |
| topic | Tyrosinase tyrosinase inhibition total phenol DPPH antioxidant activity Stachys |
| url | http://ismj.bpums.ac.ir/article-1-1142-en.html |
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