Anti-inflammatory effects of a methanol extract of Dictamnus dasycarpus Turcz. root bark on imiquimod-induced psoriasis
Abstract Background The root bark of Dictamnus dasycarpus Turcz. has been successfully used for the treatment of inflammatory skin conditions such as eczema and pruritus. However, the anti-psoriatic effect of this plant has not until now been investigated. Methods The aim of this project was to inve...
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| Format: | Article |
| Language: | English |
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BMC
2019-12-01
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| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12906-019-2767-2 |
| _version_ | 1818427708891201536 |
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| author | Minjee Choi Jun Koo Yi Si-Yong Kim Jung Hyun Ryu Jinhee Lee Wookbong Kwon Soyoung Jang Dongjun Kim MyoungOk Kim Hyungwoo Kim Sung Hyun Kim Seong-Kyoon Choi Zae Young Ryoo |
| author_facet | Minjee Choi Jun Koo Yi Si-Yong Kim Jung Hyun Ryu Jinhee Lee Wookbong Kwon Soyoung Jang Dongjun Kim MyoungOk Kim Hyungwoo Kim Sung Hyun Kim Seong-Kyoon Choi Zae Young Ryoo |
| author_sort | Minjee Choi |
| collection | DOAJ |
| description | Abstract Background The root bark of Dictamnus dasycarpus Turcz. has been successfully used for the treatment of inflammatory skin conditions such as eczema and pruritus. However, the anti-psoriatic effect of this plant has not until now been investigated. Methods The aim of this project was to investigate whether a methanol extract of Dictamnus dasycarpus Turcz. root bark (MEDD) can be used as a therapeutic agent for psoriasis in C57BL/6 mice model of imiquimod (IMQ)-induced psoriasis. IMQ and MEDD was applied to mouse skin continuously for 7 days. The skin phenotype and the levels of inflammatory cytokines, such as interferon (IFN)-γ and interleukin (IL)-17, were analyzed. The immune cell population was determined by flow cytometry, and STAT1 and 3 protein levels were measured. Results An alleviation of scaly skin phenotype, immune cell infiltration in the dermis, and epidermal hyperplasia was observed after daily MEDD treatment in the lesion-affected area. It was also found that MEDD reduced IL-17 cytokine levels decreased by 44.37% (p < 0.05), the number of IL-17-producing Th17 cells and γδT cells, and the size of the Th1 population secreting IFN-γ decreased by 45.98, 62.21, and 44.42%, respectively (p < 0.05), compared with the vehicle control group. STAT3 signals, associated with IL-17 are also reduced by MEDD. Conclusions An anti-psoriatic effect of MEDD was observed, as determined by decreased skin inflammation, reduced number of inflammatory cytokines, and a smaller population of inflammatory cells. These results contribute to the validation of the use of MEDD in the treatment of psoriasis. |
| first_indexed | 2024-12-14T14:50:02Z |
| format | Article |
| id | doaj.art-14a9b0a1580041b99db55c0eafb94e57 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-14T14:50:02Z |
| publishDate | 2019-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-14a9b0a1580041b99db55c0eafb94e572022-12-21T22:57:09ZengBMCBMC Complementary and Alternative Medicine1472-68822019-12-011911810.1186/s12906-019-2767-2Anti-inflammatory effects of a methanol extract of Dictamnus dasycarpus Turcz. root bark on imiquimod-induced psoriasisMinjee Choi0Jun Koo Yi1Si-Yong Kim2Jung Hyun Ryu3Jinhee Lee4Wookbong Kwon5Soyoung Jang6Dongjun Kim7MyoungOk Kim8Hyungwoo Kim9Sung Hyun Kim10Seong-Kyoon Choi11Zae Young Ryoo12School of Life Science, BK21 Plus KNU Creative Bioresearch Group, Kyungpook National UniversityGyeongsangbukdo Livestock Research instituteSchool of Life Science, BK21 Plus KNU Creative Bioresearch Group, Kyungpook National UniversityPusan National University School of Korean MedicineSchool of Life Science, BK21 Plus KNU Creative Bioresearch Group, Kyungpook National UniversitySchool of Life Science, BK21 Plus KNU Creative Bioresearch Group, Kyungpook National UniversitySchool of Life Science, BK21 Plus KNU Creative Bioresearch Group, Kyungpook National UniversitySchool of Life Science, BK21 Plus KNU Creative Bioresearch Group, Kyungpook National UniversitySchool of Animal Science Biotechnology, Kyungpook National UniversityPusan National University School of Korean MedicineDepartment of Bio-Medical Analysis, Korea Polytechnic CollegeCore Protein Resources Center, DGISTSchool of Life Science, BK21 Plus KNU Creative Bioresearch Group, Kyungpook National UniversityAbstract Background The root bark of Dictamnus dasycarpus Turcz. has been successfully used for the treatment of inflammatory skin conditions such as eczema and pruritus. However, the anti-psoriatic effect of this plant has not until now been investigated. Methods The aim of this project was to investigate whether a methanol extract of Dictamnus dasycarpus Turcz. root bark (MEDD) can be used as a therapeutic agent for psoriasis in C57BL/6 mice model of imiquimod (IMQ)-induced psoriasis. IMQ and MEDD was applied to mouse skin continuously for 7 days. The skin phenotype and the levels of inflammatory cytokines, such as interferon (IFN)-γ and interleukin (IL)-17, were analyzed. The immune cell population was determined by flow cytometry, and STAT1 and 3 protein levels were measured. Results An alleviation of scaly skin phenotype, immune cell infiltration in the dermis, and epidermal hyperplasia was observed after daily MEDD treatment in the lesion-affected area. It was also found that MEDD reduced IL-17 cytokine levels decreased by 44.37% (p < 0.05), the number of IL-17-producing Th17 cells and γδT cells, and the size of the Th1 population secreting IFN-γ decreased by 45.98, 62.21, and 44.42%, respectively (p < 0.05), compared with the vehicle control group. STAT3 signals, associated with IL-17 are also reduced by MEDD. Conclusions An anti-psoriatic effect of MEDD was observed, as determined by decreased skin inflammation, reduced number of inflammatory cytokines, and a smaller population of inflammatory cells. These results contribute to the validation of the use of MEDD in the treatment of psoriasis.https://doi.org/10.1186/s12906-019-2767-2Dictamnus dasycarpusInflammationPsoriasisSkinGamma delta T cellT helper cell |
| spellingShingle | Minjee Choi Jun Koo Yi Si-Yong Kim Jung Hyun Ryu Jinhee Lee Wookbong Kwon Soyoung Jang Dongjun Kim MyoungOk Kim Hyungwoo Kim Sung Hyun Kim Seong-Kyoon Choi Zae Young Ryoo Anti-inflammatory effects of a methanol extract of Dictamnus dasycarpus Turcz. root bark on imiquimod-induced psoriasis BMC Complementary and Alternative Medicine Dictamnus dasycarpus Inflammation Psoriasis Skin Gamma delta T cell T helper cell |
| title | Anti-inflammatory effects of a methanol extract of Dictamnus dasycarpus Turcz. root bark on imiquimod-induced psoriasis |
| title_full | Anti-inflammatory effects of a methanol extract of Dictamnus dasycarpus Turcz. root bark on imiquimod-induced psoriasis |
| title_fullStr | Anti-inflammatory effects of a methanol extract of Dictamnus dasycarpus Turcz. root bark on imiquimod-induced psoriasis |
| title_full_unstemmed | Anti-inflammatory effects of a methanol extract of Dictamnus dasycarpus Turcz. root bark on imiquimod-induced psoriasis |
| title_short | Anti-inflammatory effects of a methanol extract of Dictamnus dasycarpus Turcz. root bark on imiquimod-induced psoriasis |
| title_sort | anti inflammatory effects of a methanol extract of dictamnus dasycarpus turcz root bark on imiquimod induced psoriasis |
| topic | Dictamnus dasycarpus Inflammation Psoriasis Skin Gamma delta T cell T helper cell |
| url | https://doi.org/10.1186/s12906-019-2767-2 |
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