EnZolv delignification of cotton spinning mill waste and optimization of process parameters using response surface methodology (RSM)
Abstract Background EnZolv is a novel enzyme-based, eco-friendly biomass pretreatment process that has shown great potential in the field of textile engineering and biotechnology. It employs laccase from Hexagonia hirta MSF2 and 2% ethanol in the process of delignification. The process is designed t...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-03-01
|
| Series: | Biotechnology for Biofuels and Bioproducts |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-024-02473-w |
| _version_ | 1797266886832947200 |
|---|---|
| author | Santhoshkumar Subramaniam Kumutha Karunanandham A. S. M. Raja S. K. Shukla Sivakumar Uthandi |
| author_facet | Santhoshkumar Subramaniam Kumutha Karunanandham A. S. M. Raja S. K. Shukla Sivakumar Uthandi |
| author_sort | Santhoshkumar Subramaniam |
| collection | DOAJ |
| description | Abstract Background EnZolv is a novel enzyme-based, eco-friendly biomass pretreatment process that has shown great potential in the field of textile engineering and biotechnology. It employs laccase from Hexagonia hirta MSF2 and 2% ethanol in the process of delignification. The process is designed to evaluate optimal conditions to remove lignin and other impurities from cotton spinning mill waste (CSMW), without compromising the quality and strength of the fibers. CSMW is a low-cost and readily available source of cellulose, making it an ideal candidate for delignification using EnZolv. By optimizing the pretreatment conditions and harnessing the potential of enzymatic delignification, this research aims to contribute to more sustainable and efficient ways of utilizing lignocellulosic biomass in various industries for the production of biochemical and bioproducts. Results The present study emphasizes the EnZolv pretreatment in the delignification of cotton spinning mill wastes irrespective of the cellulose content. EnZolv process parameters such as, moisture content, enzyme load, incubation time, incubation temperature, and shaking speed were optimized. Under pre-optimized conditions, the percent lignin reduction was 61.34%, 61.64%, 41.85%, 35.34%, and 35.83% in blowroom droppings (BD), flat strips (FS), lickerin fly (LF), microdust (MD) and comber noils (CN), respectively. Using response surface methodology (RSM), the statistically optimized EnZolv pretreatment conditions showed lignin reduction of 59.16%, 62.88%, 48.26%, 34.64%, and 45.99% in BD, FS, LF, MD, and CN, respectively. Conclusion Traditional chemical-based pretreatment methods often involve harsh chemicals and high energy consumption, which can have detrimental effects on the environment. In contrast, EnZolv offers a greener approach by utilizing enzymes that are biodegradable and more environmentally friendly. The resulting fibers from EnZolv treatment exhibit improved properties that make them suitable for various applications. Some of the key properties include enhanced cellulose recovery, reduced lignin content, and improved biophysical and structural characteristics. These improvements can contribute to the fiber's performance and processability in different industries and future thrust for the production of cellulose-derived and lignin-derived bioproducts. Graphical Abstract |
| first_indexed | 2024-04-25T01:07:49Z |
| format | Article |
| id | doaj.art-14ade9aa09134d14bdc23439d707e108 |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-04-25T01:07:49Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-14ade9aa09134d14bdc23439d707e1082024-03-10T12:08:06ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542024-03-0117111910.1186/s13068-024-02473-wEnZolv delignification of cotton spinning mill waste and optimization of process parameters using response surface methodology (RSM)Santhoshkumar Subramaniam0Kumutha Karunanandham1A. S. M. Raja2S. K. Shukla3Sivakumar Uthandi4Biocatalysts Laboratory, Department of Agricultural Microbiology, Tamil Nadu Agricultural University (TNAU)Department of Agricultural Microbiology, Agricultural College and Research InstituteICAR-Central Institute for Research on Cotton TechnologyICAR-Central Institute for Research on Cotton TechnologyBiocatalysts Laboratory, Department of Agricultural Microbiology, Tamil Nadu Agricultural University (TNAU)Abstract Background EnZolv is a novel enzyme-based, eco-friendly biomass pretreatment process that has shown great potential in the field of textile engineering and biotechnology. It employs laccase from Hexagonia hirta MSF2 and 2% ethanol in the process of delignification. The process is designed to evaluate optimal conditions to remove lignin and other impurities from cotton spinning mill waste (CSMW), without compromising the quality and strength of the fibers. CSMW is a low-cost and readily available source of cellulose, making it an ideal candidate for delignification using EnZolv. By optimizing the pretreatment conditions and harnessing the potential of enzymatic delignification, this research aims to contribute to more sustainable and efficient ways of utilizing lignocellulosic biomass in various industries for the production of biochemical and bioproducts. Results The present study emphasizes the EnZolv pretreatment in the delignification of cotton spinning mill wastes irrespective of the cellulose content. EnZolv process parameters such as, moisture content, enzyme load, incubation time, incubation temperature, and shaking speed were optimized. Under pre-optimized conditions, the percent lignin reduction was 61.34%, 61.64%, 41.85%, 35.34%, and 35.83% in blowroom droppings (BD), flat strips (FS), lickerin fly (LF), microdust (MD) and comber noils (CN), respectively. Using response surface methodology (RSM), the statistically optimized EnZolv pretreatment conditions showed lignin reduction of 59.16%, 62.88%, 48.26%, 34.64%, and 45.99% in BD, FS, LF, MD, and CN, respectively. Conclusion Traditional chemical-based pretreatment methods often involve harsh chemicals and high energy consumption, which can have detrimental effects on the environment. In contrast, EnZolv offers a greener approach by utilizing enzymes that are biodegradable and more environmentally friendly. The resulting fibers from EnZolv treatment exhibit improved properties that make them suitable for various applications. Some of the key properties include enhanced cellulose recovery, reduced lignin content, and improved biophysical and structural characteristics. These improvements can contribute to the fiber's performance and processability in different industries and future thrust for the production of cellulose-derived and lignin-derived bioproducts. Graphical Abstracthttps://doi.org/10.1186/s13068-024-02473-wCotton spinning mill wasteEnZolvLaccaseOptimizationPretreatmentResponse surface methodology |
| spellingShingle | Santhoshkumar Subramaniam Kumutha Karunanandham A. S. M. Raja S. K. Shukla Sivakumar Uthandi EnZolv delignification of cotton spinning mill waste and optimization of process parameters using response surface methodology (RSM) Biotechnology for Biofuels and Bioproducts Cotton spinning mill waste EnZolv Laccase Optimization Pretreatment Response surface methodology |
| title | EnZolv delignification of cotton spinning mill waste and optimization of process parameters using response surface methodology (RSM) |
| title_full | EnZolv delignification of cotton spinning mill waste and optimization of process parameters using response surface methodology (RSM) |
| title_fullStr | EnZolv delignification of cotton spinning mill waste and optimization of process parameters using response surface methodology (RSM) |
| title_full_unstemmed | EnZolv delignification of cotton spinning mill waste and optimization of process parameters using response surface methodology (RSM) |
| title_short | EnZolv delignification of cotton spinning mill waste and optimization of process parameters using response surface methodology (RSM) |
| title_sort | enzolv delignification of cotton spinning mill waste and optimization of process parameters using response surface methodology rsm |
| topic | Cotton spinning mill waste EnZolv Laccase Optimization Pretreatment Response surface methodology |
| url | https://doi.org/10.1186/s13068-024-02473-w |
| work_keys_str_mv | AT santhoshkumarsubramaniam enzolvdelignificationofcottonspinningmillwasteandoptimizationofprocessparametersusingresponsesurfacemethodologyrsm AT kumuthakarunanandham enzolvdelignificationofcottonspinningmillwasteandoptimizationofprocessparametersusingresponsesurfacemethodologyrsm AT asmraja enzolvdelignificationofcottonspinningmillwasteandoptimizationofprocessparametersusingresponsesurfacemethodologyrsm AT skshukla enzolvdelignificationofcottonspinningmillwasteandoptimizationofprocessparametersusingresponsesurfacemethodologyrsm AT sivakumaruthandi enzolvdelignificationofcottonspinningmillwasteandoptimizationofprocessparametersusingresponsesurfacemethodologyrsm |