6-Shogaol Inhibits Oxidative Stress-Induced Rat Vascular Smooth Muscle Cell Apoptosis by Regulating OXR1-p53 Axis
Apoptosis of vascular smooth muscle cells (VSMCs) is closely related to the pathogenesis of cardiovascular diseases, and oxidative stress is an important cause of VSMCs’ death. Inhibiting VSMCs apoptosis is an effective preventive strategy in slowing down the development of cardiovascular disease, e...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-01-01
|
| Series: | Frontiers in Molecular Biosciences |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.808162/full |
| _version_ | 1797658087060930560 |
|---|---|
| author | Jing Liu Jing Liu Jing Liu Bin Li Bin Li Wenlian Li Taowen Pan Taowen Pan Yunpeng Diao Yunpeng Diao Fangjun Wang |
| author_facet | Jing Liu Jing Liu Jing Liu Bin Li Bin Li Wenlian Li Taowen Pan Taowen Pan Yunpeng Diao Yunpeng Diao Fangjun Wang |
| author_sort | Jing Liu |
| collection | DOAJ |
| description | Apoptosis of vascular smooth muscle cells (VSMCs) is closely related to the pathogenesis of cardiovascular diseases, and oxidative stress is an important cause of VSMCs’ death. Inhibiting VSMCs apoptosis is an effective preventive strategy in slowing down the development of cardiovascular disease, especially for atherosclerosis. In this study, we found that oxidation resistance protein 1 (OXR1), a crucial participator for responding to oxidative stress, could modulate the expression of p53, the key regulator of cell apoptosis. Our results revealed that oxidative stress promoted VSMCs apoptosis by overexpression of the OXR1-p53 axis, and 6-shogaol (6S), a major biologically active compound in ginger, could effectively attenuate cell death by preventing the upregulated expression of the OXR1-p53 axis. Quantitative proteomics analysis revealed that the degradation of p53 mediated by OXR1 might be related to the enhanced assembly of SCF ubiquitin ligase complexes, which is reported to closely relate to the modification of ubiquitination or neddylation and subsequent degradation of p53. |
| first_indexed | 2024-03-11T17:54:47Z |
| format | Article |
| id | doaj.art-14aec76fbcef43fdb21dba8e2bd2fb7b |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-03-11T17:54:47Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-14aec76fbcef43fdb21dba8e2bd2fb7b2023-10-17T17:10:20ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-01-01910.3389/fmolb.2022.8081628081626-Shogaol Inhibits Oxidative Stress-Induced Rat Vascular Smooth Muscle Cell Apoptosis by Regulating OXR1-p53 AxisJing Liu0Jing Liu1Jing Liu2Bin Li3Bin Li4Wenlian Li5Taowen Pan6Taowen Pan7Yunpeng Diao8Yunpeng Diao9Fangjun Wang10College of Pharmacy, College of Integrative Medicine, Dalian Medical University, Dalian, ChinaKey Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, ChinaDalian Anti-Infective Traditional Chinese Medicine Development Engineering Technology Research Center, Dalian, ChinaCollege of Pharmacy, College of Integrative Medicine, Dalian Medical University, Dalian, ChinaDalian Anti-Infective Traditional Chinese Medicine Development Engineering Technology Research Center, Dalian, ChinaCollege of Pharmacy, College of Integrative Medicine, Dalian Medical University, Dalian, ChinaCollege of Pharmacy, College of Integrative Medicine, Dalian Medical University, Dalian, ChinaDalian Anti-Infective Traditional Chinese Medicine Development Engineering Technology Research Center, Dalian, ChinaCollege of Pharmacy, College of Integrative Medicine, Dalian Medical University, Dalian, ChinaDalian Anti-Infective Traditional Chinese Medicine Development Engineering Technology Research Center, Dalian, ChinaKey Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, ChinaApoptosis of vascular smooth muscle cells (VSMCs) is closely related to the pathogenesis of cardiovascular diseases, and oxidative stress is an important cause of VSMCs’ death. Inhibiting VSMCs apoptosis is an effective preventive strategy in slowing down the development of cardiovascular disease, especially for atherosclerosis. In this study, we found that oxidation resistance protein 1 (OXR1), a crucial participator for responding to oxidative stress, could modulate the expression of p53, the key regulator of cell apoptosis. Our results revealed that oxidative stress promoted VSMCs apoptosis by overexpression of the OXR1-p53 axis, and 6-shogaol (6S), a major biologically active compound in ginger, could effectively attenuate cell death by preventing the upregulated expression of the OXR1-p53 axis. Quantitative proteomics analysis revealed that the degradation of p53 mediated by OXR1 might be related to the enhanced assembly of SCF ubiquitin ligase complexes, which is reported to closely relate to the modification of ubiquitination or neddylation and subsequent degradation of p53.https://www.frontiersin.org/articles/10.3389/fmolb.2022.808162/fullvascular smooth muscle cellOXR1p53oxidative stressapoptosis6-shogaol (PubChem CID: 5281794) |
| spellingShingle | Jing Liu Jing Liu Jing Liu Bin Li Bin Li Wenlian Li Taowen Pan Taowen Pan Yunpeng Diao Yunpeng Diao Fangjun Wang 6-Shogaol Inhibits Oxidative Stress-Induced Rat Vascular Smooth Muscle Cell Apoptosis by Regulating OXR1-p53 Axis Frontiers in Molecular Biosciences vascular smooth muscle cell OXR1 p53 oxidative stress apoptosis 6-shogaol (PubChem CID: 5281794) |
| title | 6-Shogaol Inhibits Oxidative Stress-Induced Rat Vascular Smooth Muscle Cell Apoptosis by Regulating OXR1-p53 Axis |
| title_full | 6-Shogaol Inhibits Oxidative Stress-Induced Rat Vascular Smooth Muscle Cell Apoptosis by Regulating OXR1-p53 Axis |
| title_fullStr | 6-Shogaol Inhibits Oxidative Stress-Induced Rat Vascular Smooth Muscle Cell Apoptosis by Regulating OXR1-p53 Axis |
| title_full_unstemmed | 6-Shogaol Inhibits Oxidative Stress-Induced Rat Vascular Smooth Muscle Cell Apoptosis by Regulating OXR1-p53 Axis |
| title_short | 6-Shogaol Inhibits Oxidative Stress-Induced Rat Vascular Smooth Muscle Cell Apoptosis by Regulating OXR1-p53 Axis |
| title_sort | 6 shogaol inhibits oxidative stress induced rat vascular smooth muscle cell apoptosis by regulating oxr1 p53 axis |
| topic | vascular smooth muscle cell OXR1 p53 oxidative stress apoptosis 6-shogaol (PubChem CID: 5281794) |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.808162/full |
| work_keys_str_mv | AT jingliu 6shogaolinhibitsoxidativestressinducedratvascularsmoothmusclecellapoptosisbyregulatingoxr1p53axis AT jingliu 6shogaolinhibitsoxidativestressinducedratvascularsmoothmusclecellapoptosisbyregulatingoxr1p53axis AT jingliu 6shogaolinhibitsoxidativestressinducedratvascularsmoothmusclecellapoptosisbyregulatingoxr1p53axis AT binli 6shogaolinhibitsoxidativestressinducedratvascularsmoothmusclecellapoptosisbyregulatingoxr1p53axis AT binli 6shogaolinhibitsoxidativestressinducedratvascularsmoothmusclecellapoptosisbyregulatingoxr1p53axis AT wenlianli 6shogaolinhibitsoxidativestressinducedratvascularsmoothmusclecellapoptosisbyregulatingoxr1p53axis AT taowenpan 6shogaolinhibitsoxidativestressinducedratvascularsmoothmusclecellapoptosisbyregulatingoxr1p53axis AT taowenpan 6shogaolinhibitsoxidativestressinducedratvascularsmoothmusclecellapoptosisbyregulatingoxr1p53axis AT yunpengdiao 6shogaolinhibitsoxidativestressinducedratvascularsmoothmusclecellapoptosisbyregulatingoxr1p53axis AT yunpengdiao 6shogaolinhibitsoxidativestressinducedratvascularsmoothmusclecellapoptosisbyregulatingoxr1p53axis AT fangjunwang 6shogaolinhibitsoxidativestressinducedratvascularsmoothmusclecellapoptosisbyregulatingoxr1p53axis |