Evaluation of cytotoxic activity from Temurui (Murraya koenigii [Linn.] Spreng) leaf extracts against HeLa cell line using MTT assay
Temurui (Murraya koenigii [Linn.] Spreng) is a local plant of Aceh. The leaves of M. koenigii are used in most of the local foods as spices. Nowadays, cancer is claimed as the second deadly disease in the world where the number of sufferers increases every year. Cervical cancer (HeLa) is one of the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Journal of Advanced Pharmaceutical Technology & Research |
| Subjects: | |
| Online Access: | http://www.japtr.org/article.asp?issn=2231-4040;year=2019;volume=10;issue=2;spage=51;epage=55;aulast=Amna |
| _version_ | 1818407931000913920 |
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| author | Ulil Amna Halimatussakdiah Puji Wahyuningsih Nurdin Saidi Rosnani Nasution |
| author_facet | Ulil Amna Halimatussakdiah Puji Wahyuningsih Nurdin Saidi Rosnani Nasution |
| author_sort | Ulil Amna |
| collection | DOAJ |
| description | Temurui (Murraya koenigii [Linn.] Spreng) is a local plant of Aceh. The leaves of M. koenigii are used in most of the local foods as spices. Nowadays, cancer is claimed as the second deadly disease in the world where the number of sufferers increases every year. Cervical cancer (HeLa) is one of the most dominant cancers that happen in developing country, including Indonesia. Some chemotherapeutic agents using synthetic drugs have been used to treat cancer, but they are relatively expensive and cause poisoning that limits their use. Based on literatures, M.koenigii serves the potential secondary metabolites that could be developed as anticancer agent. This study aims to evaluate the cytotoxic activity from three extracts of M. koenigii leaves against HeLa cell line, including hexane, ethyl acetate, and methanol. Methodology used included extraction process, phytochemical screening, and cytotoxicity evaluation. The result showed that all the three extracts demonstrated a potent cytotoxic activity for HeLa cancer cells. Hexane and ethyl acetate showed a very strong cytotoxic effect with CD50 values <1 μg/mL, whereas methanol extract showed cytotoxic effect with CD50 value of 2.25 μg/mL. These results prove the potential of M. koenigii as an anticancer agent. |
| first_indexed | 2024-12-14T09:35:40Z |
| format | Article |
| id | doaj.art-14b87237a8fe42d6822c6d0f6f6800c9 |
| institution | Directory Open Access Journal |
| issn | 2231-4040 0976-2094 |
| language | English |
| last_indexed | 2024-12-14T09:35:40Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Advanced Pharmaceutical Technology & Research |
| spelling | doaj.art-14b87237a8fe42d6822c6d0f6f6800c92022-12-21T23:07:56ZengWolters Kluwer Medknow PublicationsJournal of Advanced Pharmaceutical Technology & Research2231-40400976-20942019-01-01102515510.4103/japtr.JAPTR_373_18Evaluation of cytotoxic activity from Temurui (Murraya koenigii [Linn.] Spreng) leaf extracts against HeLa cell line using MTT assayUlil AmnaHalimatussakdiahPuji WahyuningsihNurdin SaidiRosnani NasutionTemurui (Murraya koenigii [Linn.] Spreng) is a local plant of Aceh. The leaves of M. koenigii are used in most of the local foods as spices. Nowadays, cancer is claimed as the second deadly disease in the world where the number of sufferers increases every year. Cervical cancer (HeLa) is one of the most dominant cancers that happen in developing country, including Indonesia. Some chemotherapeutic agents using synthetic drugs have been used to treat cancer, but they are relatively expensive and cause poisoning that limits their use. Based on literatures, M.koenigii serves the potential secondary metabolites that could be developed as anticancer agent. This study aims to evaluate the cytotoxic activity from three extracts of M. koenigii leaves against HeLa cell line, including hexane, ethyl acetate, and methanol. Methodology used included extraction process, phytochemical screening, and cytotoxicity evaluation. The result showed that all the three extracts demonstrated a potent cytotoxic activity for HeLa cancer cells. Hexane and ethyl acetate showed a very strong cytotoxic effect with CD50 values <1 μg/mL, whereas methanol extract showed cytotoxic effect with CD50 value of 2.25 μg/mL. These results prove the potential of M. koenigii as an anticancer agent.http://www.japtr.org/article.asp?issn=2231-4040;year=2019;volume=10;issue=2;spage=51;epage=55;aulast=AmnaAnticancercytotoxicHeLaMurraya koenigiiTemurui |
| spellingShingle | Ulil Amna Halimatussakdiah Puji Wahyuningsih Nurdin Saidi Rosnani Nasution Evaluation of cytotoxic activity from Temurui (Murraya koenigii [Linn.] Spreng) leaf extracts against HeLa cell line using MTT assay Journal of Advanced Pharmaceutical Technology & Research Anticancer cytotoxic HeLa Murraya koenigii Temurui |
| title | Evaluation of cytotoxic activity from Temurui (Murraya koenigii [Linn.] Spreng) leaf extracts against HeLa cell line using MTT assay |
| title_full | Evaluation of cytotoxic activity from Temurui (Murraya koenigii [Linn.] Spreng) leaf extracts against HeLa cell line using MTT assay |
| title_fullStr | Evaluation of cytotoxic activity from Temurui (Murraya koenigii [Linn.] Spreng) leaf extracts against HeLa cell line using MTT assay |
| title_full_unstemmed | Evaluation of cytotoxic activity from Temurui (Murraya koenigii [Linn.] Spreng) leaf extracts against HeLa cell line using MTT assay |
| title_short | Evaluation of cytotoxic activity from Temurui (Murraya koenigii [Linn.] Spreng) leaf extracts against HeLa cell line using MTT assay |
| title_sort | evaluation of cytotoxic activity from temurui murraya koenigii linn spreng leaf extracts against hela cell line using mtt assay |
| topic | Anticancer cytotoxic HeLa Murraya koenigii Temurui |
| url | http://www.japtr.org/article.asp?issn=2231-4040;year=2019;volume=10;issue=2;spage=51;epage=55;aulast=Amna |
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