Recombinant chicken interleukin-7 as a potent adjuvant increases the immunogenicity and protection of inactivated infectious bursal disease vaccine
Abstract Our previous work showed that a plasmid-based chicken interleukin-7 (chIL-7) gene expression vector possessed potent adjuvant activity for a VP2 DNA vaccine against chicken infectious bursal disease virus (IBDV). Whether recombinant chIL-7 prepared in procaryotic expression system has the a...
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| Format: | Article |
| Language: | English |
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BMC
2018-02-01
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| Series: | Veterinary Research |
| Online Access: | http://link.springer.com/article/10.1186/s13567-017-0497-3 |
| _version_ | 1819102783125585920 |
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| author | Dan Cui Jianlou Zhang Yuzhu Zuo Shanshan Huo Yonghong Zhang Liyue Wang Xiujin Li Fei Zhong |
| author_facet | Dan Cui Jianlou Zhang Yuzhu Zuo Shanshan Huo Yonghong Zhang Liyue Wang Xiujin Li Fei Zhong |
| author_sort | Dan Cui |
| collection | DOAJ |
| description | Abstract Our previous work showed that a plasmid-based chicken interleukin-7 (chIL-7) gene expression vector possessed potent adjuvant activity for a VP2 DNA vaccine against chicken infectious bursal disease virus (IBDV). Whether recombinant chIL-7 prepared in procaryotic expression system has the adjuvant activity for inactivated IBDV vaccine remains unknown. Here, we prepared recombinant chIL-7 using an E. coli expression system and analyzed its adjuvant activity for the inactivated IBDV vaccine. The results show that the recombinant chIL-7 was successfully prepared in E. coli using the pET20b vector, which possessed biological activity to stimulate mouse B lymphocyte proliferation. Co-administration of the chIL-7 with inactivated IBDV vaccine significantly increased specific serum antibody titers against IBDV, enhanced lymphocyte proliferation and IFN-γ and IL-4 productions, and increased protection against virulent IBDV infection. |
| first_indexed | 2024-12-22T01:40:03Z |
| format | Article |
| id | doaj.art-14c079a7749e483b9b428c1d52400b7a |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-12-22T01:40:03Z |
| publishDate | 2018-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-14c079a7749e483b9b428c1d52400b7a2022-12-21T18:43:15ZengBMCVeterinary Research1297-97162018-02-0149111110.1186/s13567-017-0497-3Recombinant chicken interleukin-7 as a potent adjuvant increases the immunogenicity and protection of inactivated infectious bursal disease vaccineDan Cui0Jianlou Zhang1Yuzhu Zuo2Shanshan Huo3Yonghong Zhang4Liyue Wang5Xiujin Li6Fei Zhong7Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine/College of Animal Science and Technology, Agricultural University of HebeiLaboratory of Molecular Virology and Immunology, College of Veterinary Medicine/College of Animal Science and Technology, Agricultural University of HebeiLaboratory of Molecular Virology and Immunology, College of Veterinary Medicine/College of Animal Science and Technology, Agricultural University of HebeiLaboratory of Molecular Virology and Immunology, College of Veterinary Medicine/College of Animal Science and Technology, Agricultural University of HebeiLaboratory of Molecular Virology and Immunology, College of Veterinary Medicine/College of Animal Science and Technology, Agricultural University of HebeiLaboratory of Molecular Virology and Immunology, College of Veterinary Medicine/College of Animal Science and Technology, Agricultural University of HebeiDepartment of Biotechnology, College of Environmental and Chemical Engineering, Yanshan UniversityLaboratory of Molecular Virology and Immunology, College of Veterinary Medicine/College of Animal Science and Technology, Agricultural University of HebeiAbstract Our previous work showed that a plasmid-based chicken interleukin-7 (chIL-7) gene expression vector possessed potent adjuvant activity for a VP2 DNA vaccine against chicken infectious bursal disease virus (IBDV). Whether recombinant chIL-7 prepared in procaryotic expression system has the adjuvant activity for inactivated IBDV vaccine remains unknown. Here, we prepared recombinant chIL-7 using an E. coli expression system and analyzed its adjuvant activity for the inactivated IBDV vaccine. The results show that the recombinant chIL-7 was successfully prepared in E. coli using the pET20b vector, which possessed biological activity to stimulate mouse B lymphocyte proliferation. Co-administration of the chIL-7 with inactivated IBDV vaccine significantly increased specific serum antibody titers against IBDV, enhanced lymphocyte proliferation and IFN-γ and IL-4 productions, and increased protection against virulent IBDV infection.http://link.springer.com/article/10.1186/s13567-017-0497-3 |
| spellingShingle | Dan Cui Jianlou Zhang Yuzhu Zuo Shanshan Huo Yonghong Zhang Liyue Wang Xiujin Li Fei Zhong Recombinant chicken interleukin-7 as a potent adjuvant increases the immunogenicity and protection of inactivated infectious bursal disease vaccine Veterinary Research |
| title | Recombinant chicken interleukin-7 as a potent adjuvant increases the immunogenicity and protection of inactivated infectious bursal disease vaccine |
| title_full | Recombinant chicken interleukin-7 as a potent adjuvant increases the immunogenicity and protection of inactivated infectious bursal disease vaccine |
| title_fullStr | Recombinant chicken interleukin-7 as a potent adjuvant increases the immunogenicity and protection of inactivated infectious bursal disease vaccine |
| title_full_unstemmed | Recombinant chicken interleukin-7 as a potent adjuvant increases the immunogenicity and protection of inactivated infectious bursal disease vaccine |
| title_short | Recombinant chicken interleukin-7 as a potent adjuvant increases the immunogenicity and protection of inactivated infectious bursal disease vaccine |
| title_sort | recombinant chicken interleukin 7 as a potent adjuvant increases the immunogenicity and protection of inactivated infectious bursal disease vaccine |
| url | http://link.springer.com/article/10.1186/s13567-017-0497-3 |
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