Sodium Hexametaphosphate Serves as an Inducer of Calcium Signaling
In bacteria, polymers of inorganic phosphates, particularly linear polyphosphate, are used as alternative phosphate donors for adenosine triphosphate production. A six-chain form of sodium metaphosphate, sodium hexametaphosphate (SHMP), is believed to have no physiological functions in mammalian cel...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/4/577 |
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| author | Daiki Katano Woojin Kang Yuichirou Harada Natsuko Kawano Mami Miyado Takako Saito Mio Fukuoka Mitsutoshi Yamada Kenji Miyado |
| author_facet | Daiki Katano Woojin Kang Yuichirou Harada Natsuko Kawano Mami Miyado Takako Saito Mio Fukuoka Mitsutoshi Yamada Kenji Miyado |
| author_sort | Daiki Katano |
| collection | DOAJ |
| description | In bacteria, polymers of inorganic phosphates, particularly linear polyphosphate, are used as alternative phosphate donors for adenosine triphosphate production. A six-chain form of sodium metaphosphate, sodium hexametaphosphate (SHMP), is believed to have no physiological functions in mammalian cells. In this study, we explored the possible effects of SHMP on mammalian cells, using mouse oocytes, which are useful for observing various spatiotemporal intracellular changes. Fertilization-competent oocytes were isolated from the oviducts of superovulated mice and cultured in an SHMP-containing medium. In the absence of co-incubation with sperm, SHMP-treated oocytes frequently formed pronuclei and developed into two-cell embryos owing to the increase in calcium concentration in the cytoplasm. We discovered an intriguing role for SHMP as an initiator of calcium rise in mouse oocytes, presumably in a wide variety of mammalian cells. |
| first_indexed | 2024-03-11T05:11:41Z |
| format | Article |
| id | doaj.art-14c15bf7e90d4d3c998c5948f14e1dc5 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T05:11:41Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-14c15bf7e90d4d3c998c5948f14e1dc52023-11-17T18:28:26ZengMDPI AGBiomolecules2218-273X2023-03-0113457710.3390/biom13040577Sodium Hexametaphosphate Serves as an Inducer of Calcium SignalingDaiki Katano0Woojin Kang1Yuichirou Harada2Natsuko Kawano3Mami Miyado4Takako Saito5Mio Fukuoka6Mitsutoshi Yamada7Kenji Miyado8Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashimita, Kawasaki 214-8571, JapanDepartment of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya 157-8535, JapanDepartment of Molecular Pathology, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku 192-0397, JapanDepartment of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashimita, Kawasaki 214-8571, JapanDepartment of Food and Nutrition, Beppu University, 82 Kita-Ishigaki, Beppu 874-8501, JapanDepartment of Applied Life Sciences, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, JapanDepartment of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku 160-8582, JapanDepartment of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku 160-8582, JapanDepartment of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya 157-8535, JapanIn bacteria, polymers of inorganic phosphates, particularly linear polyphosphate, are used as alternative phosphate donors for adenosine triphosphate production. A six-chain form of sodium metaphosphate, sodium hexametaphosphate (SHMP), is believed to have no physiological functions in mammalian cells. In this study, we explored the possible effects of SHMP on mammalian cells, using mouse oocytes, which are useful for observing various spatiotemporal intracellular changes. Fertilization-competent oocytes were isolated from the oviducts of superovulated mice and cultured in an SHMP-containing medium. In the absence of co-incubation with sperm, SHMP-treated oocytes frequently formed pronuclei and developed into two-cell embryos owing to the increase in calcium concentration in the cytoplasm. We discovered an intriguing role for SHMP as an initiator of calcium rise in mouse oocytes, presumably in a wide variety of mammalian cells.https://www.mdpi.com/2218-273X/13/4/577hexametaphosphatefemale reproductionoocyteparthenogenesiscalcium rise |
| spellingShingle | Daiki Katano Woojin Kang Yuichirou Harada Natsuko Kawano Mami Miyado Takako Saito Mio Fukuoka Mitsutoshi Yamada Kenji Miyado Sodium Hexametaphosphate Serves as an Inducer of Calcium Signaling Biomolecules hexametaphosphate female reproduction oocyte parthenogenesis calcium rise |
| title | Sodium Hexametaphosphate Serves as an Inducer of Calcium Signaling |
| title_full | Sodium Hexametaphosphate Serves as an Inducer of Calcium Signaling |
| title_fullStr | Sodium Hexametaphosphate Serves as an Inducer of Calcium Signaling |
| title_full_unstemmed | Sodium Hexametaphosphate Serves as an Inducer of Calcium Signaling |
| title_short | Sodium Hexametaphosphate Serves as an Inducer of Calcium Signaling |
| title_sort | sodium hexametaphosphate serves as an inducer of calcium signaling |
| topic | hexametaphosphate female reproduction oocyte parthenogenesis calcium rise |
| url | https://www.mdpi.com/2218-273X/13/4/577 |
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