Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico
Introduction: Sugarcane is an essential agricultural product for bioethanol production in Mexico. The discovery of both the bacterial community associated with this crop and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. Culture enrichment al...
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| Format: | Article |
| Language: | English |
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Universidad De La Salle Bajío
2018-05-01
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| Series: | Nova Scientia |
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| Online Access: | http://novascientia.delasalle.edu.mx/ojs/index.php/Nova/article/view/1314 |
| _version_ | 1818972963000549376 |
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| author | Nohemí Gabriela Cortés López Bernado Sachman Ruiz Fabiola Miranda Sánchez Rocío Jetzabel Alcántara-Hernández Sandra T del Moral |
| author_facet | Nohemí Gabriela Cortés López Bernado Sachman Ruiz Fabiola Miranda Sánchez Rocío Jetzabel Alcántara-Hernández Sandra T del Moral |
| author_sort | Nohemí Gabriela Cortés López |
| collection | DOAJ |
| description | Introduction: Sugarcane is an essential agricultural product for bioethanol production in Mexico. The discovery of both the bacterial community associated with this crop and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. Culture enrichment allows for the identification of the biodiversity of biological samples. The objective of this research was to identify the bacterial biodiversity related with two complex carbohydrate sources (starch and cellulose) in soils sown with sugarcane in the Papaloapan Basin in Oaxaca, Mexico via a metagenomic approach.
Method: Soil content was analyzed chemically. Liquid LB, LB-starch and LB-1% carboximetilcellulose media were inoculated with 2 g soil and cultured at 180 rpm, 37°C for 48 h. The biomass was collected and the 16S rDNA gene was amplified and a library was constructed which was analyzed by sequencing.
Results: N, K and Zn content of organic matter showed higher values than average, as opposed to P and Na, which were lower than average. In the library, 35 OTUs related to Clostridium, Bacillus, Enterococcus, Lysinibacillus and Citrobacter genera were found which could contain genes for breaking cellulose and starch.
Discussion or Conclusion: This is the first approach to identify the diversity related to starch and cellulose hydrolysis in the Papaloapan region, where the principal genera detected were Clostridium, Bacillus, Enterococcus, Citrobacter and Lysinibacillus in a soil moderately rich in organic matter. |
| first_indexed | 2024-12-20T15:16:37Z |
| format | Article |
| id | doaj.art-14c57195d7e149f59086f6bd13ebc4fa |
| institution | Directory Open Access Journal |
| issn | 2007-0705 |
| language | English |
| last_indexed | 2024-12-20T15:16:37Z |
| publishDate | 2018-05-01 |
| publisher | Universidad De La Salle Bajío |
| record_format | Article |
| series | Nova Scientia |
| spelling | doaj.art-14c57195d7e149f59086f6bd13ebc4fa2022-12-21T19:36:09ZengUniversidad De La Salle BajíoNova Scientia2007-07052018-05-011020Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of MexicoNohemí Gabriela Cortés López0Bernado Sachman Ruiz1Fabiola Miranda Sánchez2Rocío Jetzabel Alcántara-Hernández3Sandra T del Moral4Universidad del PapaloapanCentro Nacional de Investigación Disciplinaria Parasitología Veterinaria, INIFAPCentro de Ciencias Genómicas-UNAMInstituto de Geología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Av. Universidad 3000, Del. Coyoacan, 04510, Mexico City, MexicoUniversidad del PapaloapanIntroduction: Sugarcane is an essential agricultural product for bioethanol production in Mexico. The discovery of both the bacterial community associated with this crop and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. Culture enrichment allows for the identification of the biodiversity of biological samples. The objective of this research was to identify the bacterial biodiversity related with two complex carbohydrate sources (starch and cellulose) in soils sown with sugarcane in the Papaloapan Basin in Oaxaca, Mexico via a metagenomic approach. Method: Soil content was analyzed chemically. Liquid LB, LB-starch and LB-1% carboximetilcellulose media were inoculated with 2 g soil and cultured at 180 rpm, 37°C for 48 h. The biomass was collected and the 16S rDNA gene was amplified and a library was constructed which was analyzed by sequencing. Results: N, K and Zn content of organic matter showed higher values than average, as opposed to P and Na, which were lower than average. In the library, 35 OTUs related to Clostridium, Bacillus, Enterococcus, Lysinibacillus and Citrobacter genera were found which could contain genes for breaking cellulose and starch. Discussion or Conclusion: This is the first approach to identify the diversity related to starch and cellulose hydrolysis in the Papaloapan region, where the principal genera detected were Clostridium, Bacillus, Enterococcus, Citrobacter and Lysinibacillus in a soil moderately rich in organic matter.http://novascientia.delasalle.edu.mx/ojs/index.php/Nova/article/view/1314bacterial biodiversitysugarcaneenriched cultureOTUs |
| spellingShingle | Nohemí Gabriela Cortés López Bernado Sachman Ruiz Fabiola Miranda Sánchez Rocío Jetzabel Alcántara-Hernández Sandra T del Moral Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico Nova Scientia bacterial biodiversity sugarcane enriched culture OTUs |
| title | Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico |
| title_full | Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico |
| title_fullStr | Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico |
| title_full_unstemmed | Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico |
| title_short | Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico |
| title_sort | starch and cellulose related microbial diversity of soil sown with sugarcane crops in the papaloapan basin a megadiverse region of mexico |
| topic | bacterial biodiversity sugarcane enriched culture OTUs |
| url | http://novascientia.delasalle.edu.mx/ojs/index.php/Nova/article/view/1314 |
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