<i>GhCYP710A1</i> Participates in Cotton Resistance to Verticillium Wilt by Regulating Stigmasterol Synthesis and Plasma Membrane Stability
Cotton is an important economic crop. Cotton Verticillium wilt caused by <i>Verticillium dahliae</i> seriously damages production. Phytosterols play roles in plant-pathogen interaction. To explore the function and related mechanism of phytosterols in the interaction between <i>Vert...
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MDPI AG
2022-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/15/8437 |
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| author | Li Huang Guiming Li Qiaoling Wang Qian Meng Fan Xu Qian Chen Fang Liu Yulin Hu Ming Luo |
| author_facet | Li Huang Guiming Li Qiaoling Wang Qian Meng Fan Xu Qian Chen Fang Liu Yulin Hu Ming Luo |
| author_sort | Li Huang |
| collection | DOAJ |
| description | Cotton is an important economic crop. Cotton Verticillium wilt caused by <i>Verticillium dahliae</i> seriously damages production. Phytosterols play roles in plant-pathogen interaction. To explore the function and related mechanism of phytosterols in the interaction between <i>Verticillium dahliae</i> and cotton plants, and the resistance to Verticillium wilt, in this study, we analyzed the changes of sterol composition and content in cotton roots infected by <i>Verticillium dahliae</i>, and identified the sterol C22-desaturase gene <i>GhCYP710A1</i> from upland cotton. Through overexpressing and silencing the gene in cotton plant, and ectopically expressing the gene in <i>Arabidopsis</i>, we characterized the changes of sterol composition and the resistance to Verticillium wilt in transgenic plants. The infection of <i>Verticillium dahliae</i> resulted in the content of total sterol and each sterol category decreasing in cotton root. The ratio of stigmasterol to sitosterol (St/Si) increased, indicating that the conversion of sitosterol to stigmasterol was activated. Consistently, the expression level of <i>GhCYP710A1</i> was upregulated after infection. The GhCYP710A1 has the conservative domain that is essential for sterol C22-desaturase in plant and is highly expressed in root and stem, and its subcellular location is in the endoplasmic reticulum. The ectopic expression of <i>GhCYP710A1</i> gene promoted the synthesis of stigmasterol in <i>Arabidopsis</i>. The St/Si value is dose-dependent with the expression level of <i>GhCYP710A1</i> gene. Meanwhile, the resistance to Verticillium wilt of transgenic <i>Arabidopsis</i> increased and the permeability of cell membrane decreased, and the content of ROS decreased after V991 (a strain of <i>Verticillium dahliae</i>) infection. Consistently, the resistance to Verticillium wilt significantly increased in the transgenic cotton plants overexpressing <i>GhCYP710A1</i>. The membrane permeability and the colonization of V991 strain in transgenic roots were decreased. On the contrary, silencing <i>GhCYP710A1</i> resulted in the resistance to Verticillium wilt being decreased. The membrane permeability and the colonization of V991 were increased in cotton roots. The expression change of <i>GhCYP710A1</i> and the content alteration of stigmasterol lead to changes in JA signal transduction, hypersensitivity and ROS metabolism in cotton, which might be a cause for regulating the Verticillium wilt resistance of cotton plant. These results indicated that <i>GhCYP710A1</i> might be a target gene in cotton resistance breeding. |
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| spelling | doaj.art-14c5a8326a5245cba6b23ee5a82998b12023-12-01T22:57:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012315843710.3390/ijms23158437<i>GhCYP710A1</i> Participates in Cotton Resistance to Verticillium Wilt by Regulating Stigmasterol Synthesis and Plasma Membrane StabilityLi Huang0Guiming Li1Qiaoling Wang2Qian Meng3Fan Xu4Qian Chen5Fang Liu6Yulin Hu7Ming Luo8Key Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture/Biotechnology Research Center of Southwest University, Chongqing 400716, ChinaKey Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture/Biotechnology Research Center of Southwest University, Chongqing 400716, ChinaKey Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture/Biotechnology Research Center of Southwest University, Chongqing 400716, ChinaKey Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture/Biotechnology Research Center of Southwest University, Chongqing 400716, ChinaKey Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture/Biotechnology Research Center of Southwest University, Chongqing 400716, ChinaKey Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture/Biotechnology Research Center of Southwest University, Chongqing 400716, ChinaKey Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture/Biotechnology Research Center of Southwest University, Chongqing 400716, ChinaKey Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture/Biotechnology Research Center of Southwest University, Chongqing 400716, ChinaKey Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture/Biotechnology Research Center of Southwest University, Chongqing 400716, ChinaCotton is an important economic crop. Cotton Verticillium wilt caused by <i>Verticillium dahliae</i> seriously damages production. Phytosterols play roles in plant-pathogen interaction. To explore the function and related mechanism of phytosterols in the interaction between <i>Verticillium dahliae</i> and cotton plants, and the resistance to Verticillium wilt, in this study, we analyzed the changes of sterol composition and content in cotton roots infected by <i>Verticillium dahliae</i>, and identified the sterol C22-desaturase gene <i>GhCYP710A1</i> from upland cotton. Through overexpressing and silencing the gene in cotton plant, and ectopically expressing the gene in <i>Arabidopsis</i>, we characterized the changes of sterol composition and the resistance to Verticillium wilt in transgenic plants. The infection of <i>Verticillium dahliae</i> resulted in the content of total sterol and each sterol category decreasing in cotton root. The ratio of stigmasterol to sitosterol (St/Si) increased, indicating that the conversion of sitosterol to stigmasterol was activated. Consistently, the expression level of <i>GhCYP710A1</i> was upregulated after infection. The GhCYP710A1 has the conservative domain that is essential for sterol C22-desaturase in plant and is highly expressed in root and stem, and its subcellular location is in the endoplasmic reticulum. The ectopic expression of <i>GhCYP710A1</i> gene promoted the synthesis of stigmasterol in <i>Arabidopsis</i>. The St/Si value is dose-dependent with the expression level of <i>GhCYP710A1</i> gene. Meanwhile, the resistance to Verticillium wilt of transgenic <i>Arabidopsis</i> increased and the permeability of cell membrane decreased, and the content of ROS decreased after V991 (a strain of <i>Verticillium dahliae</i>) infection. Consistently, the resistance to Verticillium wilt significantly increased in the transgenic cotton plants overexpressing <i>GhCYP710A1</i>. The membrane permeability and the colonization of V991 strain in transgenic roots were decreased. On the contrary, silencing <i>GhCYP710A1</i> resulted in the resistance to Verticillium wilt being decreased. The membrane permeability and the colonization of V991 were increased in cotton roots. The expression change of <i>GhCYP710A1</i> and the content alteration of stigmasterol lead to changes in JA signal transduction, hypersensitivity and ROS metabolism in cotton, which might be a cause for regulating the Verticillium wilt resistance of cotton plant. These results indicated that <i>GhCYP710A1</i> might be a target gene in cotton resistance breeding.https://www.mdpi.com/1422-0067/23/15/8437cottonsterol C22-desaturaselipid raftJAphytosterolstigmasterol |
| spellingShingle | Li Huang Guiming Li Qiaoling Wang Qian Meng Fan Xu Qian Chen Fang Liu Yulin Hu Ming Luo <i>GhCYP710A1</i> Participates in Cotton Resistance to Verticillium Wilt by Regulating Stigmasterol Synthesis and Plasma Membrane Stability International Journal of Molecular Sciences cotton sterol C22-desaturase lipid raft JA phytosterol stigmasterol |
| title | <i>GhCYP710A1</i> Participates in Cotton Resistance to Verticillium Wilt by Regulating Stigmasterol Synthesis and Plasma Membrane Stability |
| title_full | <i>GhCYP710A1</i> Participates in Cotton Resistance to Verticillium Wilt by Regulating Stigmasterol Synthesis and Plasma Membrane Stability |
| title_fullStr | <i>GhCYP710A1</i> Participates in Cotton Resistance to Verticillium Wilt by Regulating Stigmasterol Synthesis and Plasma Membrane Stability |
| title_full_unstemmed | <i>GhCYP710A1</i> Participates in Cotton Resistance to Verticillium Wilt by Regulating Stigmasterol Synthesis and Plasma Membrane Stability |
| title_short | <i>GhCYP710A1</i> Participates in Cotton Resistance to Verticillium Wilt by Regulating Stigmasterol Synthesis and Plasma Membrane Stability |
| title_sort | i ghcyp710a1 i participates in cotton resistance to verticillium wilt by regulating stigmasterol synthesis and plasma membrane stability |
| topic | cotton sterol C22-desaturase lipid raft JA phytosterol stigmasterol |
| url | https://www.mdpi.com/1422-0067/23/15/8437 |
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