The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells.
NF-κB signaling plays an essential role in maintaining the undifferentiated state of embryonic stem (ES) cells. However, opposing roles of NF-κB have been reported in mouse and human ES cells, and the role of NF-κB in human induced pluripotent stem (iPS) cells has not yet been clarified. Here, we re...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3577895?pdf=render |
| _version_ | 1818288346957348864 |
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| author | Osamu Takase Masahiro Yoshikawa Mana Idei Junichi Hirahashi Toshiro Fujita Tsuyoshi Takato Takayuki Isagawa Genta Nagae Hirofumi Suemori Hiroyuki Aburatani Keiichi Hishikawa |
| author_facet | Osamu Takase Masahiro Yoshikawa Mana Idei Junichi Hirahashi Toshiro Fujita Tsuyoshi Takato Takayuki Isagawa Genta Nagae Hirofumi Suemori Hiroyuki Aburatani Keiichi Hishikawa |
| author_sort | Osamu Takase |
| collection | DOAJ |
| description | NF-κB signaling plays an essential role in maintaining the undifferentiated state of embryonic stem (ES) cells. However, opposing roles of NF-κB have been reported in mouse and human ES cells, and the role of NF-κB in human induced pluripotent stem (iPS) cells has not yet been clarified. Here, we report the role of NF-κB signaling in maintaining the undifferentiated state of human iPS cells. Compared with differentiated cells, undifferentiated human iPS cells showed an augmentation of NF-κB activity. During differentiation induced by the removal of feeder cells and FGF2, we observed a reduction in NF-κB activity, the expression of the undifferentiation markers Oct3/4 and Nanog, and the up-regulation of the differentiated markers WT-1 and Pax-2. The specific knockdown of NF-κB signaling using p65 siRNA also reduced the expression of Oct3/4 and Nanog and up-regulated WT-1 and Pax-2 but did not change the ES-like colony formation. Our results show that the augmentation of NF-κB signaling maintains the undifferentiated state of human iPS and suggest the importance of this signaling pathway in maintenance of human iPS cells. |
| first_indexed | 2024-12-13T01:54:56Z |
| format | Article |
| id | doaj.art-14c64e79d436496591bf9bd6022fbc1e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T01:54:56Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-14c64e79d436496591bf9bd6022fbc1e2022-12-22T00:03:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5639910.1371/journal.pone.0056399The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells.Osamu TakaseMasahiro YoshikawaMana IdeiJunichi HirahashiToshiro FujitaTsuyoshi TakatoTakayuki IsagawaGenta NagaeHirofumi SuemoriHiroyuki AburataniKeiichi HishikawaNF-κB signaling plays an essential role in maintaining the undifferentiated state of embryonic stem (ES) cells. However, opposing roles of NF-κB have been reported in mouse and human ES cells, and the role of NF-κB in human induced pluripotent stem (iPS) cells has not yet been clarified. Here, we report the role of NF-κB signaling in maintaining the undifferentiated state of human iPS cells. Compared with differentiated cells, undifferentiated human iPS cells showed an augmentation of NF-κB activity. During differentiation induced by the removal of feeder cells and FGF2, we observed a reduction in NF-κB activity, the expression of the undifferentiation markers Oct3/4 and Nanog, and the up-regulation of the differentiated markers WT-1 and Pax-2. The specific knockdown of NF-κB signaling using p65 siRNA also reduced the expression of Oct3/4 and Nanog and up-regulated WT-1 and Pax-2 but did not change the ES-like colony formation. Our results show that the augmentation of NF-κB signaling maintains the undifferentiated state of human iPS and suggest the importance of this signaling pathway in maintenance of human iPS cells.http://europepmc.org/articles/PMC3577895?pdf=render |
| spellingShingle | Osamu Takase Masahiro Yoshikawa Mana Idei Junichi Hirahashi Toshiro Fujita Tsuyoshi Takato Takayuki Isagawa Genta Nagae Hirofumi Suemori Hiroyuki Aburatani Keiichi Hishikawa The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells. PLoS ONE |
| title | The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells. |
| title_full | The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells. |
| title_fullStr | The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells. |
| title_full_unstemmed | The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells. |
| title_short | The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells. |
| title_sort | role of nf κb signaling in the maintenance of pluripotency of human induced pluripotent stem cells |
| url | http://europepmc.org/articles/PMC3577895?pdf=render |
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