Genetic relation in Capcicum annum [L.] cultivars through microsatellite markers: SSR and ISSR
Capsicum annum [L.] is one of the most economically important vegetable crops in India. In order to assess the genetic relation,DNA from thirteen capsicum cultivars were screened using inter simple sequence repeat (ISSR) and microsatellite (SSR)markers. Five ISSR primers amplified 204 reproducible b...
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| Format: | Article |
| Language: | English |
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Indian Society of Plant Breeders
2011-03-01
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| Series: | Electronic Journal of Plant Breeding |
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| Online Access: | http://sites.google.com/site/ejpb2011/vol-2-1/Vol-2-1-67-76.pdf?attredirects=0 |
| _version_ | 1818534080026771456 |
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| author | Avni S Patel, Sasidharan N., Ashish G Vala and Vinay kumar |
| author_facet | Avni S Patel, Sasidharan N., Ashish G Vala and Vinay kumar |
| author_sort | Avni S Patel, Sasidharan N., Ashish G Vala and Vinay kumar |
| collection | DOAJ |
| description | Capsicum annum [L.] is one of the most economically important vegetable crops in India. In order to assess the genetic relation,DNA from thirteen capsicum cultivars were screened using inter simple sequence repeat (ISSR) and microsatellite (SSR)markers. Five ISSR primers amplified 204 reproducible bands of which 139 were polymorphic. The percentage of polymorphicbands detected by ISSR was 100%. The highest polymorphic bands obtained by the use of primers UBC-809 (34) and UBC-66(53). A total of 1-5 alleles were detected by six SSR primers, with an average of two alleles per primer. The number of alleles perlocus ranged one (ssrCAMS-811) to five (ssrCAMS-142). The polymorphism information content (PIC) values ranged from 0.27(ssrCAMS-405) to 0.67 (ssrCAMS - 142). This study reveals the great importance of guaranteeing the differentiation of chillicultivars and the application for certification purposes |
| first_indexed | 2024-12-11T18:07:05Z |
| format | Article |
| id | doaj.art-14ca9eabf8be4521a00a952a1d8c76aa |
| institution | Directory Open Access Journal |
| issn | 0975-928X |
| language | English |
| last_indexed | 2024-12-11T18:07:05Z |
| publishDate | 2011-03-01 |
| publisher | Indian Society of Plant Breeders |
| record_format | Article |
| series | Electronic Journal of Plant Breeding |
| spelling | doaj.art-14ca9eabf8be4521a00a952a1d8c76aa2022-12-22T00:55:41ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2011-03-01216776Genetic relation in Capcicum annum [L.] cultivars through microsatellite markers: SSR and ISSRAvni S Patel, Sasidharan N., Ashish G Vala and Vinay kumarCapsicum annum [L.] is one of the most economically important vegetable crops in India. In order to assess the genetic relation,DNA from thirteen capsicum cultivars were screened using inter simple sequence repeat (ISSR) and microsatellite (SSR)markers. Five ISSR primers amplified 204 reproducible bands of which 139 were polymorphic. The percentage of polymorphicbands detected by ISSR was 100%. The highest polymorphic bands obtained by the use of primers UBC-809 (34) and UBC-66(53). A total of 1-5 alleles were detected by six SSR primers, with an average of two alleles per primer. The number of alleles perlocus ranged one (ssrCAMS-811) to five (ssrCAMS-142). The polymorphism information content (PIC) values ranged from 0.27(ssrCAMS-405) to 0.67 (ssrCAMS - 142). This study reveals the great importance of guaranteeing the differentiation of chillicultivars and the application for certification purposeshttp://sites.google.com/site/ejpb2011/vol-2-1/Vol-2-1-67-76.pdf?attredirects=0Capsicumgenetic diversityinter simple sequence repeat (ISSR)polymorphismsimple sequence repeat (SSR). |
| spellingShingle | Avni S Patel, Sasidharan N., Ashish G Vala and Vinay kumar Genetic relation in Capcicum annum [L.] cultivars through microsatellite markers: SSR and ISSR Electronic Journal of Plant Breeding Capsicum genetic diversity inter simple sequence repeat (ISSR) polymorphism simple sequence repeat (SSR). |
| title | Genetic relation in Capcicum annum [L.] cultivars through microsatellite markers: SSR and ISSR |
| title_full | Genetic relation in Capcicum annum [L.] cultivars through microsatellite markers: SSR and ISSR |
| title_fullStr | Genetic relation in Capcicum annum [L.] cultivars through microsatellite markers: SSR and ISSR |
| title_full_unstemmed | Genetic relation in Capcicum annum [L.] cultivars through microsatellite markers: SSR and ISSR |
| title_short | Genetic relation in Capcicum annum [L.] cultivars through microsatellite markers: SSR and ISSR |
| title_sort | genetic relation in capcicum annum l cultivars through microsatellite markers ssr and issr |
| topic | Capsicum genetic diversity inter simple sequence repeat (ISSR) polymorphism simple sequence repeat (SSR). |
| url | http://sites.google.com/site/ejpb2011/vol-2-1/Vol-2-1-67-76.pdf?attredirects=0 |
| work_keys_str_mv | AT avnispatelsasidharannashishgvalaandvinaykumar geneticrelationincapcicumannumlcultivarsthroughmicrosatellitemarkersssrandissr |