Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae and Contact to Animals in Estonia
We have attempted to define the prevalence and risk factors of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-Enterobacteriaceae) carriage, and to characterize antimicrobial susceptibility, beta-lactamase genes, and major types of isolated strains in volunteers, with a specific...
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MDPI AG
2020-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/8/1130 |
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| author | Kaidi Telling Age Brauer Mailis Laht Piret Kalmus Karolin Toompere Veljo Kisand Matti Maimets Maido Remm Tanel Tenson Irja Lutsar |
| author_facet | Kaidi Telling Age Brauer Mailis Laht Piret Kalmus Karolin Toompere Veljo Kisand Matti Maimets Maido Remm Tanel Tenson Irja Lutsar |
| author_sort | Kaidi Telling |
| collection | DOAJ |
| description | We have attempted to define the prevalence and risk factors of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-Enterobacteriaceae) carriage, and to characterize antimicrobial susceptibility, beta-lactamase genes, and major types of isolated strains in volunteers, with a specific focus on humans in contact with animals. Samples were collected from 207 volunteers (veterinarians, pig farmers, dog owners, etc.) and cultured on selective agar. Clonal relationships of the isolated ESBL-Enterobacteriaceae were determined by whole genome sequencing and multi-locus sequence typing. Beta-lactamases were detected using a homology search. Subjects filled in questionnaires analyzed by univariate and multiple logistic regression. Colonization with ESBL-Enterobacteriaceae was found in fecal samples of 14 individuals (6.8%; 95%CI: 3.75–11.09%). In multiple regression analysis, working as a pig farmer was a significant risk factor for ESBL-Enterobacteriaceae carriage (OR 4.8; 95%CI 1.2–19.1). The only species isolated was <i>Escherichia coli</i> that distributed into 11 sequence types. All ESBL-Enterobacteriaceae isolates were of CTX-M genotype, with the <i>bla</i>CTX-M-1 being the most prevalent and more common in pig farmers than in other groups. Despite the generally low prevalence of ESBL-Enterobacteriaceae in Estonia, the pig farmers may still pose a threat to transfer resistant microorganisms. The clinical relevance of predominant <i>bla</i>CTX-M-1 carrying <i>E. coli</i> is still unclear and needs further studies. |
| first_indexed | 2024-03-10T18:10:44Z |
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| id | doaj.art-14cae7586df4465895f81d2bb30e6c2e |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T18:10:44Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-14cae7586df4465895f81d2bb30e6c2e2023-11-20T08:04:51ZengMDPI AGMicroorganisms2076-26072020-07-0188113010.3390/microorganisms8081130Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae and Contact to Animals in EstoniaKaidi Telling0Age Brauer1Mailis Laht2Piret Kalmus3Karolin Toompere4Veljo Kisand5Matti Maimets6Maido Remm7Tanel Tenson8Irja Lutsar9Department of Microbiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411 Tartu, EstoniaInstitute of Molecular and Cell Biology, University of Tartu, 51010 Tartu, EstoniaInstitute of Technology, University of Tartu, 50411 Tartu, EstoniaThe Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, 51014 Tartu, EstoniaInstitute of Family Medicine and Public Health, University of Tartu, 50411 Tartu, EstoniaInstitute of Technology, University of Tartu, 50411 Tartu, EstoniaInstitute of Clinical Medicine, University of Tartu, 50406 Tartu, EstoniaInstitute of Molecular and Cell Biology, University of Tartu, 51010 Tartu, EstoniaInstitute of Technology, University of Tartu, 50411 Tartu, EstoniaDepartment of Microbiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411 Tartu, EstoniaWe have attempted to define the prevalence and risk factors of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-Enterobacteriaceae) carriage, and to characterize antimicrobial susceptibility, beta-lactamase genes, and major types of isolated strains in volunteers, with a specific focus on humans in contact with animals. Samples were collected from 207 volunteers (veterinarians, pig farmers, dog owners, etc.) and cultured on selective agar. Clonal relationships of the isolated ESBL-Enterobacteriaceae were determined by whole genome sequencing and multi-locus sequence typing. Beta-lactamases were detected using a homology search. Subjects filled in questionnaires analyzed by univariate and multiple logistic regression. Colonization with ESBL-Enterobacteriaceae was found in fecal samples of 14 individuals (6.8%; 95%CI: 3.75–11.09%). In multiple regression analysis, working as a pig farmer was a significant risk factor for ESBL-Enterobacteriaceae carriage (OR 4.8; 95%CI 1.2–19.1). The only species isolated was <i>Escherichia coli</i> that distributed into 11 sequence types. All ESBL-Enterobacteriaceae isolates were of CTX-M genotype, with the <i>bla</i>CTX-M-1 being the most prevalent and more common in pig farmers than in other groups. Despite the generally low prevalence of ESBL-Enterobacteriaceae in Estonia, the pig farmers may still pose a threat to transfer resistant microorganisms. The clinical relevance of predominant <i>bla</i>CTX-M-1 carrying <i>E. coli</i> is still unclear and needs further studies.https://www.mdpi.com/2076-2607/8/8/1130ESBL<i>Escherichia coli</i>healthy volunteerone healthCTX-Mwhole genome sequencing |
| spellingShingle | Kaidi Telling Age Brauer Mailis Laht Piret Kalmus Karolin Toompere Veljo Kisand Matti Maimets Maido Remm Tanel Tenson Irja Lutsar Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae and Contact to Animals in Estonia Microorganisms ESBL <i>Escherichia coli</i> healthy volunteer one health CTX-M whole genome sequencing |
| title | Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae and Contact to Animals in Estonia |
| title_full | Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae and Contact to Animals in Estonia |
| title_fullStr | Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae and Contact to Animals in Estonia |
| title_full_unstemmed | Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae and Contact to Animals in Estonia |
| title_short | Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae and Contact to Animals in Estonia |
| title_sort | characteristics of extended spectrum beta lactamase producing enterobacteriaceae and contact to animals in estonia |
| topic | ESBL <i>Escherichia coli</i> healthy volunteer one health CTX-M whole genome sequencing |
| url | https://www.mdpi.com/2076-2607/8/8/1130 |
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