A model for preservation of thymocyte-depleted thymus
DiGeorge syndrome is a disorder caused by a microdeletion on the long arm of chromosome 22. Approximately 1% of patients diagnosed with DiGeorge syndrome may have an absence of a functional thymus, which characterizes the complete form of the syndrome. These patients require urgent treatment to reco...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2023-08-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2023000100654&lng=en&tlng=en |
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| author | A.S. Dias N.R. Damaceno-Rodrigues T.M. Gimenez P.M. Oliveira M.C. Zerbini M. Carneiro-Sampaio V. Odone Filho M.B. Jatene D.M. Vasconcelos V. Rocha E.M. Novak |
| author_facet | A.S. Dias N.R. Damaceno-Rodrigues T.M. Gimenez P.M. Oliveira M.C. Zerbini M. Carneiro-Sampaio V. Odone Filho M.B. Jatene D.M. Vasconcelos V. Rocha E.M. Novak |
| author_sort | A.S. Dias |
| collection | DOAJ |
| description | DiGeorge syndrome is a disorder caused by a microdeletion on the long arm of chromosome 22. Approximately 1% of patients diagnosed with DiGeorge syndrome may have an absence of a functional thymus, which characterizes the complete form of the syndrome. These patients require urgent treatment to reconstitute T cell immunity. Thymus transplantation is a promising investigational procedure for reconstitution of thymic function in infants with congenital athymia. Here, we demonstrate a possible optimization of the preparation of thymus slices for transplantation through prior depletion of thymocytes and leukocyte cell lineages followed by cryopreservation with cryoprotective media (5% dextran FP 40, 5% Me2SO, and 5% FBS) while preserving tissue architecture. Thymus fragments were stored in liquid nitrogen at -196°C for 30 days or one year. The tissue architecture of the fragments was preserved, including the distinction between medullary thymic epithelial cells (TECs), cortical TECs, and Hassall bodies. Moreover, depleted thymus fragments cryopreserved for one year were recolonized by intrathymic injections of 3×106 thymocytes per mL, demonstrating the capability of these fragments to support T cell development. Thus, this technique opens up the possibility of freezing and storing large volumes of thymus tissue for immediate transplantation into patients with DiGeorge syndrome or atypical (Omenn-like) phenotype. |
| first_indexed | 2024-03-12T14:52:23Z |
| format | Article |
| id | doaj.art-14ceb14f44e54c68a0776c8c8802bc0c |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-03-12T14:52:23Z |
| publishDate | 2023-08-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-14ceb14f44e54c68a0776c8c8802bc0c2023-08-15T07:48:01ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2023-08-015610.1590/1414-431x2023e12647A model for preservation of thymocyte-depleted thymusA.S. Diashttps://orcid.org/0000-0002-3534-575XN.R. Damaceno-Rodrigueshttps://orcid.org/0000-0003-4287-9167T.M. Gimenezhttps://orcid.org/0000-0003-4341-8215P.M. Oliveirahttps://orcid.org/0000-0002-7694-5030M.C. Zerbinihttps://orcid.org/0000-0002-7408-9816M. Carneiro-Sampaiohttps://orcid.org/0000-0002-1924-4043V. Odone Filhohttps://orcid.org/0000-0003-4221-0551M.B. Jatenehttps://orcid.org/0000-0002-3465-0533D.M. Vasconceloshttps://orcid.org/0000-0002-3930-3354V. Rochahttps://orcid.org/0000-0003-0094-619XE.M. Novakhttps://orcid.org/0000-0002-5732-4845DiGeorge syndrome is a disorder caused by a microdeletion on the long arm of chromosome 22. Approximately 1% of patients diagnosed with DiGeorge syndrome may have an absence of a functional thymus, which characterizes the complete form of the syndrome. These patients require urgent treatment to reconstitute T cell immunity. Thymus transplantation is a promising investigational procedure for reconstitution of thymic function in infants with congenital athymia. Here, we demonstrate a possible optimization of the preparation of thymus slices for transplantation through prior depletion of thymocytes and leukocyte cell lineages followed by cryopreservation with cryoprotective media (5% dextran FP 40, 5% Me2SO, and 5% FBS) while preserving tissue architecture. Thymus fragments were stored in liquid nitrogen at -196°C for 30 days or one year. The tissue architecture of the fragments was preserved, including the distinction between medullary thymic epithelial cells (TECs), cortical TECs, and Hassall bodies. Moreover, depleted thymus fragments cryopreserved for one year were recolonized by intrathymic injections of 3×106 thymocytes per mL, demonstrating the capability of these fragments to support T cell development. Thus, this technique opens up the possibility of freezing and storing large volumes of thymus tissue for immediate transplantation into patients with DiGeorge syndrome or atypical (Omenn-like) phenotype.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2023000100654&lng=en&tlng=enThymus tissueDepletionCryopreservationDiGeorge syndrome |
| spellingShingle | A.S. Dias N.R. Damaceno-Rodrigues T.M. Gimenez P.M. Oliveira M.C. Zerbini M. Carneiro-Sampaio V. Odone Filho M.B. Jatene D.M. Vasconcelos V. Rocha E.M. Novak A model for preservation of thymocyte-depleted thymus Brazilian Journal of Medical and Biological Research Thymus tissue Depletion Cryopreservation DiGeorge syndrome |
| title | A model for preservation of thymocyte-depleted thymus |
| title_full | A model for preservation of thymocyte-depleted thymus |
| title_fullStr | A model for preservation of thymocyte-depleted thymus |
| title_full_unstemmed | A model for preservation of thymocyte-depleted thymus |
| title_short | A model for preservation of thymocyte-depleted thymus |
| title_sort | model for preservation of thymocyte depleted thymus |
| topic | Thymus tissue Depletion Cryopreservation DiGeorge syndrome |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2023000100654&lng=en&tlng=en |
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