Spermidine Affects Cardiac Function in Heart Failure Mice by Influencing the Gut Microbiota and Cardiac Galectin-3
Spermidine, which can be synthesized by the gut microbiota, can prevent cardiac hypertrophy and delay the progression to heart failure (HF). However, it is not clear whether the effect of spermidine on cardiac function is mediated by modulating the gut microbiota when HF occurs. Female HF Kunming mi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Cardiovascular Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2021.765591/full |
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| author | Yufeng Chen Zhiqin Guo Shaonan Li Shaonan Li Zhen Liu Zhen Liu Pingan Chen |
| author_facet | Yufeng Chen Zhiqin Guo Shaonan Li Shaonan Li Zhen Liu Zhen Liu Pingan Chen |
| author_sort | Yufeng Chen |
| collection | DOAJ |
| description | Spermidine, which can be synthesized by the gut microbiota, can prevent cardiac hypertrophy and delay the progression to heart failure (HF). However, it is not clear whether the effect of spermidine on cardiac function is mediated by modulating the gut microbiota when HF occurs. Female HF Kunming mice induced by transverse aortic constriction were administered spermidine (HF+S group) or its antagonist (HF+SR group). Echocardiography, messenger ribonucleic acid (RNA) and protein expression of galectin-3 in the heart, cardiomyocyte apoptosis assays and gut microbiota analysis were detected. Left ventricular end-diastolic volume and diameter (LVVd and LVDd), and left ventricular end-systolic volume and diameter in the HF+SR group were significantly enlarged compared with those in the HF group (all P < 0.05). The HF+S group had a smaller LVDd and LVVd than the HF+SR group (5.01 ± 0.67 vs. 6.13 ± 0.45 mm, P = 0.033; 121.44 ± 38.74 vs. 189.94 ± 31.42 μL, P = 0.033). The messenger RNA and protein expression of galectin-3 and the number of apoptotic cardiomyocytes increased significantly in the HF+SR group compared to the HF group. Gut microbiota analysis showed that spermidine antagonists reduced the Firmicutes/Bacteroidetes ratio and changed the microbial community richness and diversity. In conclusion, spermidine can improve cardiac function in HF, and the regulation of gut microbiota and cardiac fibrosis may be a factor in the effect of spermidine on the improvement of cardiac function. |
| first_indexed | 2024-12-14T13:45:53Z |
| format | Article |
| id | doaj.art-14d2dab2f2824d2592143a09aea413d7 |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-12-14T13:45:53Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-14d2dab2f2824d2592143a09aea413d72022-12-21T22:59:19ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2021-12-01810.3389/fcvm.2021.765591765591Spermidine Affects Cardiac Function in Heart Failure Mice by Influencing the Gut Microbiota and Cardiac Galectin-3Yufeng Chen0Zhiqin Guo1Shaonan Li2Shaonan Li3Zhen Liu4Zhen Liu5Pingan Chen6Department of Cardiology, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou, ChinaDepartment of Cardiology, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou, ChinaDepartment of Cardiology, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou, ChinaDepartment of Cardiology, Guangzhou First People's Hospital, Guangzhou, ChinaDepartment of Cardiology, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou, ChinaDepartment of Cardiology, Guangzhou First People's Hospital, Guangzhou, ChinaDepartment of Cardiology, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou, ChinaSpermidine, which can be synthesized by the gut microbiota, can prevent cardiac hypertrophy and delay the progression to heart failure (HF). However, it is not clear whether the effect of spermidine on cardiac function is mediated by modulating the gut microbiota when HF occurs. Female HF Kunming mice induced by transverse aortic constriction were administered spermidine (HF+S group) or its antagonist (HF+SR group). Echocardiography, messenger ribonucleic acid (RNA) and protein expression of galectin-3 in the heart, cardiomyocyte apoptosis assays and gut microbiota analysis were detected. Left ventricular end-diastolic volume and diameter (LVVd and LVDd), and left ventricular end-systolic volume and diameter in the HF+SR group were significantly enlarged compared with those in the HF group (all P < 0.05). The HF+S group had a smaller LVDd and LVVd than the HF+SR group (5.01 ± 0.67 vs. 6.13 ± 0.45 mm, P = 0.033; 121.44 ± 38.74 vs. 189.94 ± 31.42 μL, P = 0.033). The messenger RNA and protein expression of galectin-3 and the number of apoptotic cardiomyocytes increased significantly in the HF+SR group compared to the HF group. Gut microbiota analysis showed that spermidine antagonists reduced the Firmicutes/Bacteroidetes ratio and changed the microbial community richness and diversity. In conclusion, spermidine can improve cardiac function in HF, and the regulation of gut microbiota and cardiac fibrosis may be a factor in the effect of spermidine on the improvement of cardiac function.https://www.frontiersin.org/articles/10.3389/fcvm.2021.765591/fullheart failurespermidinemicrobiotacardiac fibrosisgalectin-3 |
| spellingShingle | Yufeng Chen Zhiqin Guo Shaonan Li Shaonan Li Zhen Liu Zhen Liu Pingan Chen Spermidine Affects Cardiac Function in Heart Failure Mice by Influencing the Gut Microbiota and Cardiac Galectin-3 Frontiers in Cardiovascular Medicine heart failure spermidine microbiota cardiac fibrosis galectin-3 |
| title | Spermidine Affects Cardiac Function in Heart Failure Mice by Influencing the Gut Microbiota and Cardiac Galectin-3 |
| title_full | Spermidine Affects Cardiac Function in Heart Failure Mice by Influencing the Gut Microbiota and Cardiac Galectin-3 |
| title_fullStr | Spermidine Affects Cardiac Function in Heart Failure Mice by Influencing the Gut Microbiota and Cardiac Galectin-3 |
| title_full_unstemmed | Spermidine Affects Cardiac Function in Heart Failure Mice by Influencing the Gut Microbiota and Cardiac Galectin-3 |
| title_short | Spermidine Affects Cardiac Function in Heart Failure Mice by Influencing the Gut Microbiota and Cardiac Galectin-3 |
| title_sort | spermidine affects cardiac function in heart failure mice by influencing the gut microbiota and cardiac galectin 3 |
| topic | heart failure spermidine microbiota cardiac fibrosis galectin-3 |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2021.765591/full |
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