The Fly Homologue of MFSD11 Is Possibly Linked to Nutrient Homeostasis and Has a Potential Role in Locomotion: A First Characterization of the Atypical Solute Carrier CG18549 in Drosophila Melanogaster
Cellular transport and function are dependent on substrate influx and efflux of various compounds. In humans, the largest superfamily of transporters is the SoLute Carriers (SLCs). Many transporters are orphans and little to nothing is known about their expression and/or function, yet they have been...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/11/1024 |
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| author | Mikaela M. Ceder Frida A. Lindberg Emelie Perland Michael J. Williams Robert Fredriksson |
| author_facet | Mikaela M. Ceder Frida A. Lindberg Emelie Perland Michael J. Williams Robert Fredriksson |
| author_sort | Mikaela M. Ceder |
| collection | DOAJ |
| description | Cellular transport and function are dependent on substrate influx and efflux of various compounds. In humans, the largest superfamily of transporters is the SoLute Carriers (SLCs). Many transporters are orphans and little to nothing is known about their expression and/or function, yet they have been assigned to a cluster called atypical SLCs. One of these atypical SLCs is MFSD11. Here we present a first in-depth characterization of the MFSD11, CG18549. By gene expression and behavior analysis on ubiquitous and brain-specific knockdown flies. <i>CG18549</i> knockdown flies were found to have altered adipokinetic hormone and adipokinteic hormone receptor expression as well as reduced vesicular monoamine transporter expression; to exhibit an altered locomotor behavior, and to have an altered reaction to stress stimuli. Furthermore, the gene expression of <i>CG18549</i> in the brain was visualized and abundant expression in both the larvae and adult brain was observed, a result that is coherent with the FlyAtlas Anatomy microarray. The exact mechanism behind the observed behaviors is not fully understood, but this study provides new insights into the expression and function of <i>CG18549</i>. Clearly, these results provide a strong example as to why it is vital to fully characterize orphan transporters and through that gain knowledge about the body during normal condition and disease. |
| first_indexed | 2024-03-10T05:24:20Z |
| format | Article |
| id | doaj.art-14da0473b00842a7ac8a54d4a8d22745 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T05:24:20Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-14da0473b00842a7ac8a54d4a8d227452023-11-22T23:47:19ZengMDPI AGInsects2075-44502021-11-011211102410.3390/insects12111024The Fly Homologue of MFSD11 Is Possibly Linked to Nutrient Homeostasis and Has a Potential Role in Locomotion: A First Characterization of the Atypical Solute Carrier CG18549 in Drosophila MelanogasterMikaela M. Ceder0Frida A. Lindberg1Emelie Perland2Michael J. Williams3Robert Fredriksson4Molecular Neuropharmacology, Department of Pharmaceutical Biosciences, Uppsala University, 751 24 Uppsala, SwedenMolecular Neuropharmacology, Department of Pharmaceutical Biosciences, Uppsala University, 751 24 Uppsala, SwedenMolecular Neuropharmacology, Department of Pharmaceutical Biosciences, Uppsala University, 751 24 Uppsala, SwedenFunctional Pharmacology, Department of Neuroscience, Uppsala University, 751 24 Uppsala, SwedenMolecular Neuropharmacology, Department of Pharmaceutical Biosciences, Uppsala University, 751 24 Uppsala, SwedenCellular transport and function are dependent on substrate influx and efflux of various compounds. In humans, the largest superfamily of transporters is the SoLute Carriers (SLCs). Many transporters are orphans and little to nothing is known about their expression and/or function, yet they have been assigned to a cluster called atypical SLCs. One of these atypical SLCs is MFSD11. Here we present a first in-depth characterization of the MFSD11, CG18549. By gene expression and behavior analysis on ubiquitous and brain-specific knockdown flies. <i>CG18549</i> knockdown flies were found to have altered adipokinetic hormone and adipokinteic hormone receptor expression as well as reduced vesicular monoamine transporter expression; to exhibit an altered locomotor behavior, and to have an altered reaction to stress stimuli. Furthermore, the gene expression of <i>CG18549</i> in the brain was visualized and abundant expression in both the larvae and adult brain was observed, a result that is coherent with the FlyAtlas Anatomy microarray. The exact mechanism behind the observed behaviors is not fully understood, but this study provides new insights into the expression and function of <i>CG18549</i>. Clearly, these results provide a strong example as to why it is vital to fully characterize orphan transporters and through that gain knowledge about the body during normal condition and disease.https://www.mdpi.com/2075-4450/12/11/1024solute carrierMFSMFSD11CG18549drosophila melanogasterlocomotion |
| spellingShingle | Mikaela M. Ceder Frida A. Lindberg Emelie Perland Michael J. Williams Robert Fredriksson The Fly Homologue of MFSD11 Is Possibly Linked to Nutrient Homeostasis and Has a Potential Role in Locomotion: A First Characterization of the Atypical Solute Carrier CG18549 in Drosophila Melanogaster Insects solute carrier MFS MFSD11 CG18549 drosophila melanogaster locomotion |
| title | The Fly Homologue of MFSD11 Is Possibly Linked to Nutrient Homeostasis and Has a Potential Role in Locomotion: A First Characterization of the Atypical Solute Carrier CG18549 in Drosophila Melanogaster |
| title_full | The Fly Homologue of MFSD11 Is Possibly Linked to Nutrient Homeostasis and Has a Potential Role in Locomotion: A First Characterization of the Atypical Solute Carrier CG18549 in Drosophila Melanogaster |
| title_fullStr | The Fly Homologue of MFSD11 Is Possibly Linked to Nutrient Homeostasis and Has a Potential Role in Locomotion: A First Characterization of the Atypical Solute Carrier CG18549 in Drosophila Melanogaster |
| title_full_unstemmed | The Fly Homologue of MFSD11 Is Possibly Linked to Nutrient Homeostasis and Has a Potential Role in Locomotion: A First Characterization of the Atypical Solute Carrier CG18549 in Drosophila Melanogaster |
| title_short | The Fly Homologue of MFSD11 Is Possibly Linked to Nutrient Homeostasis and Has a Potential Role in Locomotion: A First Characterization of the Atypical Solute Carrier CG18549 in Drosophila Melanogaster |
| title_sort | fly homologue of mfsd11 is possibly linked to nutrient homeostasis and has a potential role in locomotion a first characterization of the atypical solute carrier cg18549 in drosophila melanogaster |
| topic | solute carrier MFS MFSD11 CG18549 drosophila melanogaster locomotion |
| url | https://www.mdpi.com/2075-4450/12/11/1024 |
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