In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia
Introduction: Over 170 municipalities in Colombia have been invaded by Lissachatina fulica, an African snail that can carry larvae of nematodes of interest in human and veterinary health. Nematodes enter the host snail as larvae L1 and then change to L2 and L3, the infectious form for vertebrates. O...
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| Format: | Article |
| Language: | English |
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Instituto Nacional de Salud
2018-08-01
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| Series: | Biomédica: revista del Instituto Nacional de Salud |
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| Online Access: | https://www.revistabiomedica.org/index.php/biomedica/article/view/3408 |
| _version_ | 1818011780774887424 |
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| author | Nelly Solfania Heredia Ann Sabrina Ávila Luz Elena Velásquez |
| author_facet | Nelly Solfania Heredia Ann Sabrina Ávila Luz Elena Velásquez |
| author_sort | Nelly Solfania Heredia |
| collection | DOAJ |
| description | Introduction: Over 170 municipalities in Colombia have been invaded by Lissachatina fulica, an African snail that can carry larvae of nematodes of interest in human and veterinary health. Nematodes enter the host snail as larvae L1 and then change to L2 and L3, the infectious form for vertebrates.
Objective: To standardize culture in vitro of L3 carried by L. fulica from Santa Fe de Antioquia.
Materials and methods: Between July and November, 2014, 10 snails were collected, killed, and conserved with HCl 0.7%. Larvae were recovered using the Baermann technique and cultured for 36 days in Schneider, DMEM and RPMI media, with and without SFB 20% and distilled water with SFB 20%. Replacements were made every 36 hours; larvae were measured with an ocular micrometer on a microscope. Summary statistics were estimated; box and whisker plots were made; the t Student test was performed in SPSS 18™. A p-value below 0.05 was assumed as significant.
Results: Fifty per cent of the larvae survived. The highest survival and growth was 85% in supplemented DMEM. The final average length of larvae in supplemented media exceeded the initial one. There were significant differences between the average length of larvae cultured in supplemented media and the initial length. The initial width of larvae did not change.
Conclusions: The best medium for the culture of L3 larvae was supplemented DMEM. The length provided more information than the width for the larval growth evaluation. The larvae studied did not correspond to Angiostrongylus cantonensis, A. costaricensis or Aelurostrongylus abstrusus. |
| first_indexed | 2024-04-14T06:12:53Z |
| format | Article |
| id | doaj.art-14da65a9637445ff877dd66b3dda1694 |
| institution | Directory Open Access Journal |
| issn | 0120-4157 0120-4157 |
| language | English |
| last_indexed | 2024-04-14T06:12:53Z |
| publishDate | 2018-08-01 |
| publisher | Instituto Nacional de Salud |
| record_format | Article |
| series | Biomédica: revista del Instituto Nacional de Salud |
| spelling | doaj.art-14da65a9637445ff877dd66b3dda16942022-12-22T02:08:19ZengInstituto Nacional de SaludBiomédica: revista del Instituto Nacional de Salud0120-41570120-41572018-08-01380242910.7705/biomedica.v38i3.34082118In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de AntioquiaNelly Solfania Heredia0Ann Sabrina Ávila1Luz Elena Velásquez2Grupo de Microbiología Ambiental, Escuela de Microbiología, Universidad de Antioquia, Medellín, ColombiaGrupo de Microbiología Ambiental, Escuela de Microbiología, Universidad de Antioquia, Medellín, ColombiaGrupo de Microbiología Ambiental, Escuela de Microbiología, Universidad de Antioquia, Medellín, Colombia Programa de Estudio y Control de Enfermedades Tropicales, PECET, Universidad de Antioquia, Medellín, ColombiaIntroduction: Over 170 municipalities in Colombia have been invaded by Lissachatina fulica, an African snail that can carry larvae of nematodes of interest in human and veterinary health. Nematodes enter the host snail as larvae L1 and then change to L2 and L3, the infectious form for vertebrates. Objective: To standardize culture in vitro of L3 carried by L. fulica from Santa Fe de Antioquia. Materials and methods: Between July and November, 2014, 10 snails were collected, killed, and conserved with HCl 0.7%. Larvae were recovered using the Baermann technique and cultured for 36 days in Schneider, DMEM and RPMI media, with and without SFB 20% and distilled water with SFB 20%. Replacements were made every 36 hours; larvae were measured with an ocular micrometer on a microscope. Summary statistics were estimated; box and whisker plots were made; the t Student test was performed in SPSS 18™. A p-value below 0.05 was assumed as significant. Results: Fifty per cent of the larvae survived. The highest survival and growth was 85% in supplemented DMEM. The final average length of larvae in supplemented media exceeded the initial one. There were significant differences between the average length of larvae cultured in supplemented media and the initial length. The initial width of larvae did not change. Conclusions: The best medium for the culture of L3 larvae was supplemented DMEM. The length provided more information than the width for the larval growth evaluation. The larvae studied did not correspond to Angiostrongylus cantonensis, A. costaricensis or Aelurostrongylus abstrusus.https://www.revistabiomedica.org/index.php/biomedica/article/view/3408Nematodasnailsculture mediain vitro techniquesColombia |
| spellingShingle | Nelly Solfania Heredia Ann Sabrina Ávila Luz Elena Velásquez In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia Biomédica: revista del Instituto Nacional de Salud Nematoda snails culture media in vitro techniques Colombia |
| title | In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia |
| title_full | In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia |
| title_fullStr | In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia |
| title_full_unstemmed | In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia |
| title_short | In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia |
| title_sort | in vitro culture of l3 larvae of nematodes obtained from the african giant snail lissachatina fulica mollusca gastropoda in santa fe de antioquia |
| topic | Nematoda snails culture media in vitro techniques Colombia |
| url | https://www.revistabiomedica.org/index.php/biomedica/article/view/3408 |
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