The Physical, Chemical, and the Biological Stability Test on Liposome EPC-TEL 2.5 as the Newest Drug Delivery Systems (Drug Carrier), In Vitro and In Vivo
This experiment is carried out in order to improve the stability of the Liposome EPC-TEL 2.5 physically, chemically, and biologically. As a new formula, this liposome that has contained phosphatidylcholine from egg yolk=EPC and Tetra-ether Lipid (TEL) from membrane of Sulfolobus acidocaldarius or Th...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Indonesia
2010-10-01
|
| Series: | Makara Journal of Health Research |
| Subjects: | |
| Online Access: | http://journal.ui.ac.id/index.php/health/article/view/264 |
| _version_ | 1797718132922515456 |
|---|---|
| author | Ernie Purwaningsih Wawaimuli Arozal Sri Widia A. Jusman |
| author_facet | Ernie Purwaningsih Wawaimuli Arozal Sri Widia A. Jusman |
| author_sort | Ernie Purwaningsih |
| collection | DOAJ |
| description | This experiment is carried out in order to improve the stability of the Liposome EPC-TEL 2.5 physically, chemically, and biologically. As a new formula, this liposome that has contained phosphatidylcholine from egg yolk=EPC and Tetra-ether Lipid (TEL) from membrane of Sulfolobus acidocaldarius or Thermoplasma acidophilum had never been tested on their stability. The stability of liposome to carry the drug into the targeted cells or organs is required for determining the therapeutic dose of the drugs. Physically, the test was done by measuring the amount and diameter of liposome after incubating at 4º C, at room temperature, and 37º C. Chemically, the test was also done using the same parameters after introduction of chemical solution of NaCl, CaCl2; MgCl2; at the pH of 5; 7; 9. The measurements was carried out on day 1; 7; and month 1; 2; and 3. Biologically, liposome EPC-TEL 2.5 was injected Intra-Peritoneally to mice to detect the degradation of TEL in their liver, at the minute of 0; 30 ; 60 ; the hour of 2; 4; and 8. The results of these tests were shown that liposome EPC-TEL 2.5 was stable until the last month of 1 at 4º C and 37º C on physical stability test; more stable at the chemical solution of NaCl and CaCl2 at the pH of 5 and 7 until two months; and TEL was degradable in liver of mice. |
| first_indexed | 2024-03-12T08:46:30Z |
| format | Article |
| id | doaj.art-14dcdc237eb64a5291d1b06d91139383 |
| institution | Directory Open Access Journal |
| issn | 2356-3664 2356-3656 |
| language | English |
| last_indexed | 2024-03-12T08:46:30Z |
| publishDate | 2010-10-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Makara Journal of Health Research |
| spelling | doaj.art-14dcdc237eb64a5291d1b06d911393832023-09-02T16:25:42ZengUniversitas IndonesiaMakara Journal of Health Research2356-36642356-36562010-10-01112848910.7454/msk.v11i2.264256The Physical, Chemical, and the Biological Stability Test on Liposome EPC-TEL 2.5 as the Newest Drug Delivery Systems (Drug Carrier), In Vitro and In VivoErnie Purwaningsih0Wawaimuli Arozal1Sri Widia A. Jusman2Department of Medical Pharmacy, Faculty of Medicine, Universitas Indonesia, Jakarta 10430Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta 10430Department of Biochemistry and Biomolecular Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta 10430This experiment is carried out in order to improve the stability of the Liposome EPC-TEL 2.5 physically, chemically, and biologically. As a new formula, this liposome that has contained phosphatidylcholine from egg yolk=EPC and Tetra-ether Lipid (TEL) from membrane of Sulfolobus acidocaldarius or Thermoplasma acidophilum had never been tested on their stability. The stability of liposome to carry the drug into the targeted cells or organs is required for determining the therapeutic dose of the drugs. Physically, the test was done by measuring the amount and diameter of liposome after incubating at 4º C, at room temperature, and 37º C. Chemically, the test was also done using the same parameters after introduction of chemical solution of NaCl, CaCl2; MgCl2; at the pH of 5; 7; 9. The measurements was carried out on day 1; 7; and month 1; 2; and 3. Biologically, liposome EPC-TEL 2.5 was injected Intra-Peritoneally to mice to detect the degradation of TEL in their liver, at the minute of 0; 30 ; 60 ; the hour of 2; 4; and 8. The results of these tests were shown that liposome EPC-TEL 2.5 was stable until the last month of 1 at 4º C and 37º C on physical stability test; more stable at the chemical solution of NaCl and CaCl2 at the pH of 5 and 7 until two months; and TEL was degradable in liver of mice.http://journal.ui.ac.id/index.php/health/article/view/264liposomeEPC-TEL2.5stabilitynanotechnology |
| spellingShingle | Ernie Purwaningsih Wawaimuli Arozal Sri Widia A. Jusman The Physical, Chemical, and the Biological Stability Test on Liposome EPC-TEL 2.5 as the Newest Drug Delivery Systems (Drug Carrier), In Vitro and In Vivo Makara Journal of Health Research liposome EPC-TEL2.5 stability nanotechnology |
| title | The Physical, Chemical, and the Biological Stability Test on Liposome EPC-TEL 2.5 as the Newest Drug Delivery Systems (Drug Carrier), In Vitro and In Vivo |
| title_full | The Physical, Chemical, and the Biological Stability Test on Liposome EPC-TEL 2.5 as the Newest Drug Delivery Systems (Drug Carrier), In Vitro and In Vivo |
| title_fullStr | The Physical, Chemical, and the Biological Stability Test on Liposome EPC-TEL 2.5 as the Newest Drug Delivery Systems (Drug Carrier), In Vitro and In Vivo |
| title_full_unstemmed | The Physical, Chemical, and the Biological Stability Test on Liposome EPC-TEL 2.5 as the Newest Drug Delivery Systems (Drug Carrier), In Vitro and In Vivo |
| title_short | The Physical, Chemical, and the Biological Stability Test on Liposome EPC-TEL 2.5 as the Newest Drug Delivery Systems (Drug Carrier), In Vitro and In Vivo |
| title_sort | physical chemical and the biological stability test on liposome epc tel 2 5 as the newest drug delivery systems drug carrier in vitro and in vivo |
| topic | liposome EPC-TEL2.5 stability nanotechnology |
| url | http://journal.ui.ac.id/index.php/health/article/view/264 |
| work_keys_str_mv | AT erniepurwaningsih thephysicalchemicalandthebiologicalstabilitytestonliposomeepctel25asthenewestdrugdeliverysystemsdrugcarrierinvitroandinvivo AT wawaimuliarozal thephysicalchemicalandthebiologicalstabilitytestonliposomeepctel25asthenewestdrugdeliverysystemsdrugcarrierinvitroandinvivo AT sriwidiaajusman thephysicalchemicalandthebiologicalstabilitytestonliposomeepctel25asthenewestdrugdeliverysystemsdrugcarrierinvitroandinvivo AT erniepurwaningsih physicalchemicalandthebiologicalstabilitytestonliposomeepctel25asthenewestdrugdeliverysystemsdrugcarrierinvitroandinvivo AT wawaimuliarozal physicalchemicalandthebiologicalstabilitytestonliposomeepctel25asthenewestdrugdeliverysystemsdrugcarrierinvitroandinvivo AT sriwidiaajusman physicalchemicalandthebiologicalstabilitytestonliposomeepctel25asthenewestdrugdeliverysystemsdrugcarrierinvitroandinvivo |