Development of Microsatellites for <i>Verbenoxylum reitzii</i> (Verbenaceae), a Tree Endemic to the Brazilian Atlantic Forest
Premise of the study: Microsatellite markers were developed for <i>Verbenoxylum reitzii</i> (Verbenaceae), a tree endemic to the Brazilian Atlantic Forest, to investigate their usefulness in population genetic studies. The loci were tested for cross-amplification in the related genera &l...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-07-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1300005 |
| _version_ | 1818326258129305600 |
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| author | Verônica A. Thode Alice Backes Geraldo Mader Raquel Kriedt Sandro L. Bonatto Loreta B. Freitas |
| author_facet | Verônica A. Thode Alice Backes Geraldo Mader Raquel Kriedt Sandro L. Bonatto Loreta B. Freitas |
| author_sort | Verônica A. Thode |
| collection | DOAJ |
| description | Premise of the study: Microsatellite markers were developed for <i>Verbenoxylum reitzii</i> (Verbenaceae), a tree endemic to the Brazilian Atlantic Forest, to investigate their usefulness in population genetic studies. The loci were tested for cross-amplification in the related genera <i>Recordia</i> and <i>Duranta</i>.
Methods and Results: Eleven polymorphic microsatellite markers were isolated from an enriched library of <i>V. reitzii</i> and characterized. The primers were tested on 60 individuals from three populations of this species. The number of alleles per locus ranged from two to 11, and the observed and expected heterozygosities varied from 0.0 to 1.0 and from 0.088 to 0.758, respectively. Ten loci successfully amplified in <i>R. boliviana</i> and all failed in <i>D. vestita</i>.
Conclusions: Our results suggest the usefulness of the microsatellite loci developed here to access genetic variability for phylogeographic and population genetic studies in <i>V. reitzii</i>, which are important for the conservation of this rare species. |
| first_indexed | 2024-12-13T11:57:31Z |
| format | Article |
| id | doaj.art-14def4feed184bf795004601ddf52bbe |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-13T11:57:31Z |
| publishDate | 2013-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-14def4feed184bf795004601ddf52bbe2022-12-21T23:47:10ZengWileyApplications in Plant Sciences2168-04502013-07-0118130000510.3732/apps.1300005Development of Microsatellites for <i>Verbenoxylum reitzii</i> (Verbenaceae), a Tree Endemic to the Brazilian Atlantic ForestVerônica A. Thode0Alice Backes1Geraldo Mader2Raquel Kriedt3Sandro L. Bonatto4Loreta B. Freitas5Programa de Pós-Graduação em Botânica, Universidade Federal do Rio Grande do Sul, Bento Gonçalves 9500, Porto Alegre,Brazil; Laboratory of Molecular Evolution, Department of Genetics, Universidade Federal do Rio Grande do Sul, PO Box 15053, Porto Alegre, BrazilLaboratory of Molecular Evolution, Department of Genetics, Universidade Federal do Rio Grande do Sul, PO Box 15053, Porto Alegre, BrazilLaboratory of Molecular Evolution, Department of Genetics, Universidade Federal do Rio Grande do Sul, PO Box 15053, Porto Alegre, BrazilLaboratory of Molecular Evolution, Department of Genetics, Universidade Federal do Rio Grande do Sul, PO Box 15053, Porto Alegre, BrazilGenomic and Molecular Biology Laboratory, Pontifícia Universidade Católica do Rio Grande do Sul, Ipiranga 6681, Porto Alegre, BrazilPrograma de Pós-Graduação em Botânica, Universidade Federal do Rio Grande do Sul, Bento Gonçalves 9500, Porto Alegre, Brazil; Laboratory of Molecular Evolution, Department of Genetics, Universidade Federal do Rio Grande do Sul, PO Box 15053, Porto Alegre, BrazilPremise of the study: Microsatellite markers were developed for <i>Verbenoxylum reitzii</i> (Verbenaceae), a tree endemic to the Brazilian Atlantic Forest, to investigate their usefulness in population genetic studies. The loci were tested for cross-amplification in the related genera <i>Recordia</i> and <i>Duranta</i>. Methods and Results: Eleven polymorphic microsatellite markers were isolated from an enriched library of <i>V. reitzii</i> and characterized. The primers were tested on 60 individuals from three populations of this species. The number of alleles per locus ranged from two to 11, and the observed and expected heterozygosities varied from 0.0 to 1.0 and from 0.088 to 0.758, respectively. Ten loci successfully amplified in <i>R. boliviana</i> and all failed in <i>D. vestita</i>. Conclusions: Our results suggest the usefulness of the microsatellite loci developed here to access genetic variability for phylogeographic and population genetic studies in <i>V. reitzii</i>, which are important for the conservation of this rare species.http://www.bioone.org/doi/full/10.3732/apps.1300005cross-amplificationmicrosatelliteRecordiaVerbenaceaeVerbenoxylumVerbenoxylum reitzii |
| spellingShingle | Verônica A. Thode Alice Backes Geraldo Mader Raquel Kriedt Sandro L. Bonatto Loreta B. Freitas Development of Microsatellites for <i>Verbenoxylum reitzii</i> (Verbenaceae), a Tree Endemic to the Brazilian Atlantic Forest Applications in Plant Sciences cross-amplification microsatellite Recordia Verbenaceae Verbenoxylum Verbenoxylum reitzii |
| title | Development of Microsatellites for <i>Verbenoxylum reitzii</i> (Verbenaceae), a Tree Endemic to the Brazilian Atlantic Forest |
| title_full | Development of Microsatellites for <i>Verbenoxylum reitzii</i> (Verbenaceae), a Tree Endemic to the Brazilian Atlantic Forest |
| title_fullStr | Development of Microsatellites for <i>Verbenoxylum reitzii</i> (Verbenaceae), a Tree Endemic to the Brazilian Atlantic Forest |
| title_full_unstemmed | Development of Microsatellites for <i>Verbenoxylum reitzii</i> (Verbenaceae), a Tree Endemic to the Brazilian Atlantic Forest |
| title_short | Development of Microsatellites for <i>Verbenoxylum reitzii</i> (Verbenaceae), a Tree Endemic to the Brazilian Atlantic Forest |
| title_sort | development of microsatellites for i verbenoxylum reitzii i verbenaceae a tree endemic to the brazilian atlantic forest |
| topic | cross-amplification microsatellite Recordia Verbenaceae Verbenoxylum Verbenoxylum reitzii |
| url | http://www.bioone.org/doi/full/10.3732/apps.1300005 |
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