Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults
The gut microbiome plays a major role in the maintenance of human health. Characterizing the taxonomy and metabolic functions of the human gut microbiome is necessary for enhancing health. Here, we analyzed the metagenomic sequencing, assembly and construction of a meta-gene catalogue of the human g...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Genes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4425/12/3/331 |
| _version_ | 1797395278706245632 |
|---|---|
| author | Nachon Raethong Massalin Nakphaichit Narissara Suratannon Witida Sathitkowitchai Wanlapa Weerapakorn Suttipun Keawsompong Wanwipa Vongsangnak |
| author_facet | Nachon Raethong Massalin Nakphaichit Narissara Suratannon Witida Sathitkowitchai Wanlapa Weerapakorn Suttipun Keawsompong Wanwipa Vongsangnak |
| author_sort | Nachon Raethong |
| collection | DOAJ |
| description | The gut microbiome plays a major role in the maintenance of human health. Characterizing the taxonomy and metabolic functions of the human gut microbiome is necessary for enhancing health. Here, we analyzed the metagenomic sequencing, assembly and construction of a meta-gene catalogue of the human gut microbiome with the overall aim of investigating the taxonomy and metabolic functions of the gut microbiome in Thai adults. As a result, the integrative analysis of 16S rRNA gene and whole metagenome shotgun (WMGS) sequencing data revealed that the dominant gut bacterial families were <i>Lachnospiraceae</i> and <i>Ruminococcaceae</i> of the Firmicutes phylum. Consistently, across 3.8 million (M) genes annotated from 163.5 gigabases (Gb) of WMGS sequencing data, a significant number of genes associated with carbohydrate metabolism of the dominant bacterial families were identified. Further identification of bacterial community-wide metabolic functions promisingly highlighted the importance of <i>Roseburia</i> and <i>Faecalibacterium</i> involvement in central carbon metabolism, sugar utilization and metabolism towards butyrate biosynthesis. This work presents an initial study of shotgun metagenomics in a Thai population-based cohort in a developing Southeast Asian country. |
| first_indexed | 2024-03-09T00:33:10Z |
| format | Article |
| id | doaj.art-14df3d5a02ce4faaaa8fcf7f0fa1314c |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T00:33:10Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-14df3d5a02ce4faaaa8fcf7f0fa1314c2023-12-11T18:21:58ZengMDPI AGGenes2073-44252021-02-0112333110.3390/genes12030331Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai AdultsNachon Raethong0Massalin Nakphaichit1Narissara Suratannon2Witida Sathitkowitchai3Wanlapa Weerapakorn4Suttipun Keawsompong5Wanwipa Vongsangnak6Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, ThailandDepartment of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, ThailandPediatric Allergy & Clinical Immunology Research Unit, Division of Allergy and Immunology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, The Thai Red Cross Society, Bangkok 10330, ThailandDepartment of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, ThailandPediatric Allergy & Clinical Immunology Research Unit, Division of Allergy and Immunology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, The Thai Red Cross Society, Bangkok 10330, ThailandDepartment of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, ThailandDepartment of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, ThailandThe gut microbiome plays a major role in the maintenance of human health. Characterizing the taxonomy and metabolic functions of the human gut microbiome is necessary for enhancing health. Here, we analyzed the metagenomic sequencing, assembly and construction of a meta-gene catalogue of the human gut microbiome with the overall aim of investigating the taxonomy and metabolic functions of the gut microbiome in Thai adults. As a result, the integrative analysis of 16S rRNA gene and whole metagenome shotgun (WMGS) sequencing data revealed that the dominant gut bacterial families were <i>Lachnospiraceae</i> and <i>Ruminococcaceae</i> of the Firmicutes phylum. Consistently, across 3.8 million (M) genes annotated from 163.5 gigabases (Gb) of WMGS sequencing data, a significant number of genes associated with carbohydrate metabolism of the dominant bacterial families were identified. Further identification of bacterial community-wide metabolic functions promisingly highlighted the importance of <i>Roseburia</i> and <i>Faecalibacterium</i> involvement in central carbon metabolism, sugar utilization and metabolism towards butyrate biosynthesis. This work presents an initial study of shotgun metagenomics in a Thai population-based cohort in a developing Southeast Asian country.https://www.mdpi.com/2073-4425/12/3/331gut microbiomeThai adultswhole metagenome shotgun (WMGS) sequencingmeta-gene cataloguemetabolic functions |
| spellingShingle | Nachon Raethong Massalin Nakphaichit Narissara Suratannon Witida Sathitkowitchai Wanlapa Weerapakorn Suttipun Keawsompong Wanwipa Vongsangnak Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults Genes gut microbiome Thai adults whole metagenome shotgun (WMGS) sequencing meta-gene catalogue metabolic functions |
| title | Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults |
| title_full | Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults |
| title_fullStr | Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults |
| title_full_unstemmed | Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults |
| title_short | Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults |
| title_sort | analysis of human gut microbiome taxonomy and metabolic functions in thai adults |
| topic | gut microbiome Thai adults whole metagenome shotgun (WMGS) sequencing meta-gene catalogue metabolic functions |
| url | https://www.mdpi.com/2073-4425/12/3/331 |
| work_keys_str_mv | AT nachonraethong analysisofhumangutmicrobiometaxonomyandmetabolicfunctionsinthaiadults AT massalinnakphaichit analysisofhumangutmicrobiometaxonomyandmetabolicfunctionsinthaiadults AT narissarasuratannon analysisofhumangutmicrobiometaxonomyandmetabolicfunctionsinthaiadults AT witidasathitkowitchai analysisofhumangutmicrobiometaxonomyandmetabolicfunctionsinthaiadults AT wanlapaweerapakorn analysisofhumangutmicrobiometaxonomyandmetabolicfunctionsinthaiadults AT suttipunkeawsompong analysisofhumangutmicrobiometaxonomyandmetabolicfunctionsinthaiadults AT wanwipavongsangnak analysisofhumangutmicrobiometaxonomyandmetabolicfunctionsinthaiadults |