The Effect of LRP-1 Gene and Mir-205 on Cardiovascular Disease in Iraqi Patients with Familial Hypercholesterolemia
Familial hypercholesterolemia (FH) is a genetic disorder characterized by autosomal inheritance in genes related to LDL-C metabolism, with the major clinical features of hyper-LDL-cholesterolemia and premature coronary artery disease. (LRP-1) is a member of the LDLR family. It is a membrane receptor...
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| Format: | Article |
| Language: | English |
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University of Technology, Baghdad
2021-09-01
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| Series: | Journal of Applied Sciences and Nanotechnology |
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| Online Access: | https://jasn.uotechnology.edu.iq/article_15545_6c8afa668f1615751952d50345134464.pdf |
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| author | Zinah Ghareeb Hiba AlKhafaji Mohanad Al-Saedi |
| author_facet | Zinah Ghareeb Hiba AlKhafaji Mohanad Al-Saedi |
| author_sort | Zinah Ghareeb |
| collection | DOAJ |
| description | Familial hypercholesterolemia (FH) is a genetic disorder characterized by autosomal inheritance in genes related to LDL-C metabolism, with the major clinical features of hyper-LDL-cholesterolemia and premature coronary artery disease. (LRP-1) is a member of the LDLR family. It is a membrane receptor with scavenging and signaling properties. LRP-1 interacts with a wide range of extracellular ligands as well as intracellular scaffolding and signaling proteins, which makes it important in crucial clinical circumstances like cardiovascular disease, cancer, and neurological illnesses. Mir-205 uses these molecules as biomarker for cardiovascular diseases. This study aims to measure gene expression for the LPR-1 gene and its relationship to the development of cardiovascular disease in familial hypercholesterolemia and non-familial hypercholesterolemia. Also, it studies the indication whether mir-205 regulates the action of the LRP-1 gene in terms of increasing or decreasing gene expression. However, the available methods for measuring LRP1 levels are direct and quantitative using Poly Chain Reaction (RT-PCR) in real time, not at its ends. In the present study, blood was isolated from 150 individuals distributed into three groups: Group 1 included: 50 samples from a healthy group; Group 2: 50 samples from non-Familial hypercholesterolemia patients; Group 3:50 samples Familial hyperchol-esterolemia patients. The results showed that LRP1 protein expression was significantly reduced in patients with F.H compared with normal control in a small cohort from an Iraqi population. This pilot study suggests that the reduced LRP1 protein expression may be associated with cardiovascular disease progression. |
| first_indexed | 2024-12-22T02:02:48Z |
| format | Article |
| id | doaj.art-14e5b9e7f48c44b28c92cdbd8f723598 |
| institution | Directory Open Access Journal |
| issn | 2788-6867 |
| language | English |
| last_indexed | 2024-12-22T02:02:48Z |
| publishDate | 2021-09-01 |
| publisher | University of Technology, Baghdad |
| record_format | Article |
| series | Journal of Applied Sciences and Nanotechnology |
| spelling | doaj.art-14e5b9e7f48c44b28c92cdbd8f7235982022-12-21T18:42:36ZengUniversity of Technology, BaghdadJournal of Applied Sciences and Nanotechnology2788-68672021-09-0113243110.53293/jasn.2021.3691.103615545The Effect of LRP-1 Gene and Mir-205 on Cardiovascular Disease in Iraqi Patients with Familial HypercholesterolemiaZinah Ghareeb0Hiba AlKhafaji1Mohanad Al-Saedi2Department of Applied Sciences, University of Technology, IraqDepartment of Applied Sciences, University of Technology, IraqIraqi Hereditary Company, IraqFamilial hypercholesterolemia (FH) is a genetic disorder characterized by autosomal inheritance in genes related to LDL-C metabolism, with the major clinical features of hyper-LDL-cholesterolemia and premature coronary artery disease. (LRP-1) is a member of the LDLR family. It is a membrane receptor with scavenging and signaling properties. LRP-1 interacts with a wide range of extracellular ligands as well as intracellular scaffolding and signaling proteins, which makes it important in crucial clinical circumstances like cardiovascular disease, cancer, and neurological illnesses. Mir-205 uses these molecules as biomarker for cardiovascular diseases. This study aims to measure gene expression for the LPR-1 gene and its relationship to the development of cardiovascular disease in familial hypercholesterolemia and non-familial hypercholesterolemia. Also, it studies the indication whether mir-205 regulates the action of the LRP-1 gene in terms of increasing or decreasing gene expression. However, the available methods for measuring LRP1 levels are direct and quantitative using Poly Chain Reaction (RT-PCR) in real time, not at its ends. In the present study, blood was isolated from 150 individuals distributed into three groups: Group 1 included: 50 samples from a healthy group; Group 2: 50 samples from non-Familial hypercholesterolemia patients; Group 3:50 samples Familial hyperchol-esterolemia patients. The results showed that LRP1 protein expression was significantly reduced in patients with F.H compared with normal control in a small cohort from an Iraqi population. This pilot study suggests that the reduced LRP1 protein expression may be associated with cardiovascular disease progression.https://jasn.uotechnology.edu.iq/article_15545_6c8afa668f1615751952d50345134464.pdffamilial hypercholesterolemialrp-1 genmir-205 genecardiovascular disease |
| spellingShingle | Zinah Ghareeb Hiba AlKhafaji Mohanad Al-Saedi The Effect of LRP-1 Gene and Mir-205 on Cardiovascular Disease in Iraqi Patients with Familial Hypercholesterolemia Journal of Applied Sciences and Nanotechnology familial hypercholesterolemia lrp-1 gen mir-205 gene cardiovascular disease |
| title | The Effect of LRP-1 Gene and Mir-205 on Cardiovascular Disease in Iraqi Patients with Familial Hypercholesterolemia |
| title_full | The Effect of LRP-1 Gene and Mir-205 on Cardiovascular Disease in Iraqi Patients with Familial Hypercholesterolemia |
| title_fullStr | The Effect of LRP-1 Gene and Mir-205 on Cardiovascular Disease in Iraqi Patients with Familial Hypercholesterolemia |
| title_full_unstemmed | The Effect of LRP-1 Gene and Mir-205 on Cardiovascular Disease in Iraqi Patients with Familial Hypercholesterolemia |
| title_short | The Effect of LRP-1 Gene and Mir-205 on Cardiovascular Disease in Iraqi Patients with Familial Hypercholesterolemia |
| title_sort | effect of lrp 1 gene and mir 205 on cardiovascular disease in iraqi patients with familial hypercholesterolemia |
| topic | familial hypercholesterolemia lrp-1 gen mir-205 gene cardiovascular disease |
| url | https://jasn.uotechnology.edu.iq/article_15545_6c8afa668f1615751952d50345134464.pdf |
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