The potential value of LC-MS non-targeted metabonomics in the diagnosis of follicular thyroid carcinoma
BackgroundTo explore the metabolic differences of follicular thyroid carcinoma (FTC) by metabonomics, to find potential biomarkers for the diagnosis of FTC, and to explore the pathogenesis and diagnosis and treatment strategies of FTC.MethodThe metabonomics of 15 patients with FTC and 15 patients wi...
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.1076548/full |
| _version_ | 1797979286724935680 |
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| author | Jiali Qin Yang Yang Wei Du Wei Du Gang Li Yao Wu Ruihua Luo Shanting Liu Jie Fan |
| author_facet | Jiali Qin Yang Yang Wei Du Wei Du Gang Li Yao Wu Ruihua Luo Shanting Liu Jie Fan |
| author_sort | Jiali Qin |
| collection | DOAJ |
| description | BackgroundTo explore the metabolic differences of follicular thyroid carcinoma (FTC) by metabonomics, to find potential biomarkers for the diagnosis of FTC, and to explore the pathogenesis and diagnosis and treatment strategies of FTC.MethodThe metabonomics of 15 patients with FTC and 15 patients with follicular thyroid nodules(FTN) treated in Henan Cancer Hospital were analyzed by liquid chromatography-mass spectrometry (LC-MS).ResultsThe analysis showed that the metabolite profiles of FTC tissues could be well distinguished from those of control tissues, and 6 kinds of lipids were identified respectively, including lysophosphatidic acid(LysoPA) [LysoPA(0:0/18:0),LysoPA(0:0/18:2(9Z,12Z)],LysoPA[20:4(8Z,11Z,14Z,17Z)/0:0)]; phosphatidic acid(PA) [PA(20:3(8Z,11Z,14Z)/0:0),PA(20:4(5Z,8Z,11Z,14Z)/0:0),PA(20:5(5Z,8Z,11Z,14Z,17Z)/0:0)]; lysophosphatidylcholine(LPC) [LPC(18:1),LPC(16:0),LPC[16:1(9Z)/0:0],LPC(17:0),LPC[22:4(7Z,10Z,13Z,16Z),LPC(20:2(11Z,14Z); phosphatidylcholine(PC)(PC(14:0/0:0),PC(16:0/0:0); sphingomyelin(SM) (d18:0/12:0); fatty acid(FA)(18:1(OH3)]. There are 2 kinds of amino acids, including L-glutamate,L-glutamine.There are 3 other metabolites, including retinol,flavin adenine dinucleotide,androsterone glucuronide.Lipid metabolites are the main metabolites in these metabolites.The metabolic pathways related to FTC were analyzed by KEGG and HMDB, and 9 metabolic pathways were found, including 4 amino acid related metabolic pathways, 1 lipid metabolic pathways and 4 other related pathways.ConclusionThere are significant differences in many metabonomic characteristics between FTC and FTN, suggesting that these metabolites can be used as potential biomarkers. Further study found that LysoPA and its analogues can be used as biomarkers in the early diagnosis of FTC.It may be related to the abnormal metabolism of phospholipase D (PLD), the key enzyme of LysoPA synthesis caused by RAS pathway. At the same time, it was found that the metabolic pathway of amino acids and lipids was the main metabolic pathway of FTC. The abnormality of LysoPA may be the cause of follicular tumor carcinogenesis caused by lipid metabolic pathway. |
| first_indexed | 2024-04-11T05:36:35Z |
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| id | doaj.art-14e9a9714a8946b698dc3bb8891d4864 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-04-11T05:36:35Z |
| publishDate | 2022-12-01 |
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| series | Frontiers in Oncology |
| spelling | doaj.art-14e9a9714a8946b698dc3bb8891d48642022-12-22T11:37:56ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-12-011210.3389/fonc.2022.10765481076548The potential value of LC-MS non-targeted metabonomics in the diagnosis of follicular thyroid carcinomaJiali Qin0Yang Yang1Wei Du2Wei Du3Gang Li4Yao Wu5Ruihua Luo6Shanting Liu7Jie Fan8Department of Head Neck and Thyroid Surgery, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan, ChinaDepartment of Head Neck and Thyroid Surgery, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan, ChinaDepartment of Head Neck and Thyroid Surgery, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan, ChinaDepartment of Anatomy, Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Head Neck and Thyroid Surgery, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan, ChinaDepartment of Head Neck and Thyroid Surgery, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan, ChinaDepartment of Head Neck and Thyroid Surgery, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan, ChinaDepartment of Head Neck and Thyroid Surgery, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan, ChinaDepartment of Head Neck and Thyroid Surgery, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan, ChinaBackgroundTo explore the metabolic differences of follicular thyroid carcinoma (FTC) by metabonomics, to find potential biomarkers for the diagnosis of FTC, and to explore the pathogenesis and diagnosis and treatment strategies of FTC.MethodThe metabonomics of 15 patients with FTC and 15 patients with follicular thyroid nodules(FTN) treated in Henan Cancer Hospital were analyzed by liquid chromatography-mass spectrometry (LC-MS).ResultsThe analysis showed that the metabolite profiles of FTC tissues could be well distinguished from those of control tissues, and 6 kinds of lipids were identified respectively, including lysophosphatidic acid(LysoPA) [LysoPA(0:0/18:0),LysoPA(0:0/18:2(9Z,12Z)],LysoPA[20:4(8Z,11Z,14Z,17Z)/0:0)]; phosphatidic acid(PA) [PA(20:3(8Z,11Z,14Z)/0:0),PA(20:4(5Z,8Z,11Z,14Z)/0:0),PA(20:5(5Z,8Z,11Z,14Z,17Z)/0:0)]; lysophosphatidylcholine(LPC) [LPC(18:1),LPC(16:0),LPC[16:1(9Z)/0:0],LPC(17:0),LPC[22:4(7Z,10Z,13Z,16Z),LPC(20:2(11Z,14Z); phosphatidylcholine(PC)(PC(14:0/0:0),PC(16:0/0:0); sphingomyelin(SM) (d18:0/12:0); fatty acid(FA)(18:1(OH3)]. There are 2 kinds of amino acids, including L-glutamate,L-glutamine.There are 3 other metabolites, including retinol,flavin adenine dinucleotide,androsterone glucuronide.Lipid metabolites are the main metabolites in these metabolites.The metabolic pathways related to FTC were analyzed by KEGG and HMDB, and 9 metabolic pathways were found, including 4 amino acid related metabolic pathways, 1 lipid metabolic pathways and 4 other related pathways.ConclusionThere are significant differences in many metabonomic characteristics between FTC and FTN, suggesting that these metabolites can be used as potential biomarkers. Further study found that LysoPA and its analogues can be used as biomarkers in the early diagnosis of FTC.It may be related to the abnormal metabolism of phospholipase D (PLD), the key enzyme of LysoPA synthesis caused by RAS pathway. At the same time, it was found that the metabolic pathway of amino acids and lipids was the main metabolic pathway of FTC. The abnormality of LysoPA may be the cause of follicular tumor carcinogenesis caused by lipid metabolic pathway.https://www.frontiersin.org/articles/10.3389/fonc.2022.1076548/fullfollicular thyroid carcinomametabonomicslipid metabolitesRASLysoPA |
| spellingShingle | Jiali Qin Yang Yang Wei Du Wei Du Gang Li Yao Wu Ruihua Luo Shanting Liu Jie Fan The potential value of LC-MS non-targeted metabonomics in the diagnosis of follicular thyroid carcinoma Frontiers in Oncology follicular thyroid carcinoma metabonomics lipid metabolites RAS LysoPA |
| title | The potential value of LC-MS non-targeted metabonomics in the diagnosis of follicular thyroid carcinoma |
| title_full | The potential value of LC-MS non-targeted metabonomics in the diagnosis of follicular thyroid carcinoma |
| title_fullStr | The potential value of LC-MS non-targeted metabonomics in the diagnosis of follicular thyroid carcinoma |
| title_full_unstemmed | The potential value of LC-MS non-targeted metabonomics in the diagnosis of follicular thyroid carcinoma |
| title_short | The potential value of LC-MS non-targeted metabonomics in the diagnosis of follicular thyroid carcinoma |
| title_sort | potential value of lc ms non targeted metabonomics in the diagnosis of follicular thyroid carcinoma |
| topic | follicular thyroid carcinoma metabonomics lipid metabolites RAS LysoPA |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.1076548/full |
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