Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians
Objective : In this article, we report on the intradermal Alcian blue staining method for tracing the meridians of acupuncture. Methods : 1% Alcian blue solution was injected into acupoints by using a 0.5mL insulin syringe with a 31-gauge needle, then the skin was incised and was observed under a s...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Pharmacopuncture Institute
2008-06-01
|
| Series: | Journal of Pharmacopuncture |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3831/KPI.2008.11.2.005 |
| _version_ | 1819153329014439936 |
|---|---|
| author | Baeckkyoung Sung Min-Su Kim2 Vyacheslav Ogay Dae-In Kang Kwang-Sup Soh |
| author_facet | Baeckkyoung Sung Min-Su Kim2 Vyacheslav Ogay Dae-In Kang Kwang-Sup Soh |
| author_sort | Baeckkyoung Sung |
| collection | DOAJ |
| description | Objective : In this article, we report on the intradermal Alcian blue staining method for tracing the meridians of acupuncture.
Methods : 1% Alcian blue solution was injected into acupoints by using a 0.5mL insulin syringe with a 31-gauge needle, then the skin was incised and was observed under a stereoscopic microscope. The specimens were examined by using immunohistochemical methods and were observed under a confocal laser scanning microscope.
Results : A threadlike structure, which was visualized with Alcian blue, existed in dermis layer and proceeded to hypodermis. In this structure, characteristic alignments of rod- shaped nuclei and 1-2μm sized DNA granules were observed. Furthermore, abundant blood capillary plexuses, peripheral nerve endings, and a corpusclelike structure(about 300μm in diameter) were visualized in the skin tissues of acupoints.
Conclusion : It was concluded that the specific threadlike and corpuscle-like structures corresponded to superficial Bonghan
duct and corpuscle, respectively. |
| first_indexed | 2024-12-22T15:03:27Z |
| format | Article |
| id | doaj.art-14e9e1eee7bb4def96d98f51b535588f |
| institution | Directory Open Access Journal |
| issn | 2093-6966 2234-6856 |
| language | English |
| last_indexed | 2024-12-22T15:03:27Z |
| publishDate | 2008-06-01 |
| publisher | Korean Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Pharmacopuncture |
| spelling | doaj.art-14e9e1eee7bb4def96d98f51b535588f2022-12-21T18:22:03ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662234-68562008-06-0111251210.3831/KPI.2008.11.2.005Intradermal Alcian-Blue Injection Method to Trace Acupuncture MeridiansBaeckkyoung Sung0Min-Su Kim21Vyacheslav Ogay2Dae-In Kang3Kwang-Sup Soh4Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, Seoul 151-747, KoreaDepartment of Veterinary Surgery, College of Veterinary Medicine, Chonbuk National University, Jeonju 664-14, KoreaBiomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, Seoul 151-747, KoreaKorean Pharmacopuncture InstituteBiomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, Seoul 151-747, KoreaObjective : In this article, we report on the intradermal Alcian blue staining method for tracing the meridians of acupuncture. Methods : 1% Alcian blue solution was injected into acupoints by using a 0.5mL insulin syringe with a 31-gauge needle, then the skin was incised and was observed under a stereoscopic microscope. The specimens were examined by using immunohistochemical methods and were observed under a confocal laser scanning microscope. Results : A threadlike structure, which was visualized with Alcian blue, existed in dermis layer and proceeded to hypodermis. In this structure, characteristic alignments of rod- shaped nuclei and 1-2μm sized DNA granules were observed. Furthermore, abundant blood capillary plexuses, peripheral nerve endings, and a corpusclelike structure(about 300μm in diameter) were visualized in the skin tissues of acupoints. Conclusion : It was concluded that the specific threadlike and corpuscle-like structures corresponded to superficial Bonghan duct and corpuscle, respectively.http://dx.doi.org/10.3831/KPI.2008.11.2.005AcupointIntradermal injectionAlcian blueTracingSuperficial Bonghan duct and corpuscleImmunohistochemistryDrug delivery |
| spellingShingle | Baeckkyoung Sung Min-Su Kim2 Vyacheslav Ogay Dae-In Kang Kwang-Sup Soh Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians Journal of Pharmacopuncture Acupoint Intradermal injection Alcian blue Tracing Superficial Bonghan duct and corpuscle Immunohistochemistry Drug delivery |
| title | Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians |
| title_full | Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians |
| title_fullStr | Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians |
| title_full_unstemmed | Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians |
| title_short | Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians |
| title_sort | intradermal alcian blue injection method to trace acupuncture meridians |
| topic | Acupoint Intradermal injection Alcian blue Tracing Superficial Bonghan duct and corpuscle Immunohistochemistry Drug delivery |
| url | http://dx.doi.org/10.3831/KPI.2008.11.2.005 |
| work_keys_str_mv | AT baeckkyoungsung intradermalalcianblueinjectionmethodtotraceacupuncturemeridians AT minsukim2 intradermalalcianblueinjectionmethodtotraceacupuncturemeridians AT vyacheslavogay intradermalalcianblueinjectionmethodtotraceacupuncturemeridians AT daeinkang intradermalalcianblueinjectionmethodtotraceacupuncturemeridians AT kwangsupsoh intradermalalcianblueinjectionmethodtotraceacupuncturemeridians |