Evaluation of bactericidal effects of silver hydrosol nanotherapeutics against Enterococcus faecium 1449 drug resistant biofilms
IntroductionSilver (Ag) nanoparticles (NPs) are well documented for their broad-spectrum bactericidal effects. This study aimed to test the effect of bioactive Ag-hydrosol NPs on drug-resistant E. faecium 1449 strain and explore the use of artificial intelligence (AI) for automated detection of the...
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| Format: | Article |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1095156/full |
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| author | Alya Limayem Alya Limayem Mausam Mehta Mausam Mehta Natalie Kondos Natalie Kondos Divya Kaushal Farhat Binte Azam Sriram Chellappan Nan Qin Qingyu Zhou |
| author_facet | Alya Limayem Alya Limayem Mausam Mehta Mausam Mehta Natalie Kondos Natalie Kondos Divya Kaushal Farhat Binte Azam Sriram Chellappan Nan Qin Qingyu Zhou |
| author_sort | Alya Limayem |
| collection | DOAJ |
| description | IntroductionSilver (Ag) nanoparticles (NPs) are well documented for their broad-spectrum bactericidal effects. This study aimed to test the effect of bioactive Ag-hydrosol NPs on drug-resistant E. faecium 1449 strain and explore the use of artificial intelligence (AI) for automated detection of the bacteria.MethodsThe formation of E. faecium 1449 biofilms in the absence and presence of Ag-hydrosol NPs at different concentrations ranging from 12.4 mg/L to 123 mg/L was evaluated using a 3-dimentional culture system. The biofilm reduction was evaluated using the confocal microscopy in addition to the Transmission Electronic Microscopy (TEM) visualization and spectrofluorimetric quantification using a Biotek Synergy Neo2 microplate reader. The cytotoxicity of the NPs was evaluated in human nasal epithelial cells using the MTT assay. The AI technique based on Fast Regional Convolutional Neural Network architecture was used for the automated detection of the bacteria.ResultsTreatment with Ag-hydrosol NPs at concentrations ranging from 12.4 mg/L to 123 mg/L resulted in 78.09% to 95.20% of biofilm reduction. No statistically significant difference in biofilm reduction was found among different batches of Ag-hydrosol NPs. Quantitative concentration-response relationship analysis indicated that Ag-hydrosol NPs exhibited a relative high anti-biofilm activity and low cytotoxicity with an average EC50 and TC50 values of 0.0333 and 6.55 mg/L, respectively, yielding an average therapeutic index value of 197. The AI-assisted TEM image analysis allowed automated detection of E. faecium 1449 with 97% ~ 99% accuracy.DiscussionConclusively, the bioactive Ag-hydrosol NP is a promising nanotherapeutic agent against drug-resistant pathogens. The AI-assisted TEM image analysis was developed with the potential to assess its treatment effect. |
| first_indexed | 2024-04-10T23:42:12Z |
| format | Article |
| id | doaj.art-14ea5908fa2747ee8111c600f47a6373 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-10T23:42:12Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-14ea5908fa2747ee8111c600f47a63732023-01-11T06:19:00ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-01-011210.3389/fcimb.2022.10951561095156Evaluation of bactericidal effects of silver hydrosol nanotherapeutics against Enterococcus faecium 1449 drug resistant biofilmsAlya Limayem0Alya Limayem1Mausam Mehta2Mausam Mehta3Natalie Kondos4Natalie Kondos5Divya Kaushal6Farhat Binte Azam7Sriram Chellappan8Nan Qin9Qingyu Zhou10Department of Biology, College of Arts & Sciences, University of North Florida, Jacksonville, FL, United StatesDepartment of Pharmaceutical Sciences, Graduate Program, Taneja College of Pharmacy, University of South Florida, Tampa, FL, United StatesDepartment of Pharmaceutical Sciences, Graduate Program, Taneja College of Pharmacy, University of South Florida, Tampa, FL, United StatesMorsani College of Medicine, University of South Florida, Tampa, FL, United StatesDepartment of Pharmaceutical Sciences, Graduate Program, Taneja College of Pharmacy, University of South Florida, Tampa, FL, United StatesMorsani College of Medicine, University of South Florida, Tampa, FL, United StatesDepartment of Pharmaceutical Sciences, Graduate Program, Taneja College of Pharmacy, University of South Florida, Tampa, FL, United StatesDepartment of Computer Science & Engineering, College of Engineering, University of South Florida, Tampa, FL, United StatesDepartment of Computer Science & Engineering, College of Engineering, University of South Florida, Tampa, FL, United StatesDepartment of R&D and Analytical Services, Natural Immunogenics Corporation, Sarasota, FL, United StatesDepartment of Pharmaceutical Sciences, Graduate Program, Taneja College of Pharmacy, University of South Florida, Tampa, FL, United StatesIntroductionSilver (Ag) nanoparticles (NPs) are well documented for their broad-spectrum bactericidal effects. This study aimed to test the effect of bioactive Ag-hydrosol NPs on drug-resistant E. faecium 1449 strain and explore the use of artificial intelligence (AI) for automated detection of the bacteria.MethodsThe formation of E. faecium 1449 biofilms in the absence and presence of Ag-hydrosol NPs at different concentrations ranging from 12.4 mg/L to 123 mg/L was evaluated using a 3-dimentional culture system. The biofilm reduction was evaluated using the confocal microscopy in addition to the Transmission Electronic Microscopy (TEM) visualization and spectrofluorimetric quantification using a Biotek Synergy Neo2 microplate reader. The cytotoxicity of the NPs was evaluated in human nasal epithelial cells using the MTT assay. The AI technique based on Fast Regional Convolutional Neural Network architecture was used for the automated detection of the bacteria.ResultsTreatment with Ag-hydrosol NPs at concentrations ranging from 12.4 mg/L to 123 mg/L resulted in 78.09% to 95.20% of biofilm reduction. No statistically significant difference in biofilm reduction was found among different batches of Ag-hydrosol NPs. Quantitative concentration-response relationship analysis indicated that Ag-hydrosol NPs exhibited a relative high anti-biofilm activity and low cytotoxicity with an average EC50 and TC50 values of 0.0333 and 6.55 mg/L, respectively, yielding an average therapeutic index value of 197. The AI-assisted TEM image analysis allowed automated detection of E. faecium 1449 with 97% ~ 99% accuracy.DiscussionConclusively, the bioactive Ag-hydrosol NP is a promising nanotherapeutic agent against drug-resistant pathogens. The AI-assisted TEM image analysis was developed with the potential to assess its treatment effect.https://www.frontiersin.org/articles/10.3389/fcimb.2022.1095156/fullmulti-drug resistanceE. faeciumbioactive silver hydrosol nanoparticlesbactericidal treatmentcytotoxicityartificial intelligence |
| spellingShingle | Alya Limayem Alya Limayem Mausam Mehta Mausam Mehta Natalie Kondos Natalie Kondos Divya Kaushal Farhat Binte Azam Sriram Chellappan Nan Qin Qingyu Zhou Evaluation of bactericidal effects of silver hydrosol nanotherapeutics against Enterococcus faecium 1449 drug resistant biofilms Frontiers in Cellular and Infection Microbiology multi-drug resistance E. faecium bioactive silver hydrosol nanoparticles bactericidal treatment cytotoxicity artificial intelligence |
| title | Evaluation of bactericidal effects of silver hydrosol nanotherapeutics against Enterococcus faecium 1449 drug resistant biofilms |
| title_full | Evaluation of bactericidal effects of silver hydrosol nanotherapeutics against Enterococcus faecium 1449 drug resistant biofilms |
| title_fullStr | Evaluation of bactericidal effects of silver hydrosol nanotherapeutics against Enterococcus faecium 1449 drug resistant biofilms |
| title_full_unstemmed | Evaluation of bactericidal effects of silver hydrosol nanotherapeutics against Enterococcus faecium 1449 drug resistant biofilms |
| title_short | Evaluation of bactericidal effects of silver hydrosol nanotherapeutics against Enterococcus faecium 1449 drug resistant biofilms |
| title_sort | evaluation of bactericidal effects of silver hydrosol nanotherapeutics against enterococcus faecium 1449 drug resistant biofilms |
| topic | multi-drug resistance E. faecium bioactive silver hydrosol nanoparticles bactericidal treatment cytotoxicity artificial intelligence |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1095156/full |
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