Effect of sulforaphane on antioxidant capacity and mitochondrial function of hepatocytes in vitro and in rats with hepatic oxidative stress
Objective To investigate the effect of sulforaphane (SFN) on oxidative stress and mitochondrial injury in hepatocytes. Methods We examined malondialdehyde (MDA) content by ELISA in the liver tissues and the protein expression of the antioxidant enzymes including hemeoxygenase-1 (HO-1), NADH dehydrog...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Third Military Medical University
2020-01-01
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| Series: | Di-san junyi daxue xuebao |
| Subjects: | |
| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/201908073.htm |
| _version_ | 1818676260970168320 |
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| author | LEI Peng TIAN Sicong TENG Chunying SUN Yao SHAN Yujuan LI Baolong |
| author_facet | LEI Peng TIAN Sicong TENG Chunying SUN Yao SHAN Yujuan LI Baolong |
| author_sort | LEI Peng |
| collection | DOAJ |
| description | Objective To investigate the effect of sulforaphane (SFN) on oxidative stress and mitochondrial injury in hepatocytes. Methods We examined malondialdehyde (MDA) content by ELISA in the liver tissues and the protein expression of the antioxidant enzymes including hemeoxygenase-1 (HO-1), NADH dehydrogenase quinone 1 (NQO1) and glutathione S-transferase (GST) using Western blotting in the liver tissues from a rat model of hepatic oxidative stress established by high-fat diet. We also assessed the level of reactive oxygen species (ROS) and mitochondrial membrane protential by fluorescence microscopy, tested glutathione (GSH) and superoxide dismutase (SOD) levels using ELISA, and detected mitochondrial DNA (mtDNA) content using real-time quantitative PCR in cultured hepatocytes (HHL-5 cells) under fatty acid-induced oxidative stress. Results In the rat model of hepatic oxidative stress, SFN significantly reduced high-fat diet-induced elevation of MDA level (P < 0.05) and up-regulated the protein expression of the antioxidant enzymes in the liver tissues (P < 0.05). In cultured HHL-5 cells, SFN obviously reduced free fatty acid-induced elevation of ROS level (P < 0.05) and increased GSH content (P < 0.05), SOD level (P < 0.05), mitochondrial membrane potential (P < 0.05), and the content of mtDNA (P < 0.05). Conclusion SFN can enhance the antioxidant capacity of hepatocytes to improve high-fat diet-induced hepatic oxidative stress in rats and provide protection of mitochondrial function in the hepatocytes. |
| first_indexed | 2024-12-17T08:40:39Z |
| format | Article |
| id | doaj.art-14ef0c1cc0c14300afc704f166328234 |
| institution | Directory Open Access Journal |
| issn | 1000-5404 |
| language | zho |
| last_indexed | 2024-12-17T08:40:39Z |
| publishDate | 2020-01-01 |
| publisher | Editorial Office of Journal of Third Military Medical University |
| record_format | Article |
| series | Di-san junyi daxue xuebao |
| spelling | doaj.art-14ef0c1cc0c14300afc704f1663282342022-12-21T21:56:21ZzhoEditorial Office of Journal of Third Military Medical UniversityDi-san junyi daxue xuebao1000-54042020-01-0142214114710.16016/j.1000-5404.201908073Effect of sulforaphane on antioxidant capacity and mitochondrial function of hepatocytes in vitro and in rats with hepatic oxidative stressLEI Peng0TIAN Sicong1TENG Chunying2SUN Yao3SHAN Yujuan4LI Baolong5Faculty of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang Province, 150001Faculty of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang Province, 150001Faculty of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang Province, 150001Faculty of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang Province, 150001Faculty of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang Province, 150001Center of Drug Safety and Evaluation, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, 150040, ChinaObjective To investigate the effect of sulforaphane (SFN) on oxidative stress and mitochondrial injury in hepatocytes. Methods We examined malondialdehyde (MDA) content by ELISA in the liver tissues and the protein expression of the antioxidant enzymes including hemeoxygenase-1 (HO-1), NADH dehydrogenase quinone 1 (NQO1) and glutathione S-transferase (GST) using Western blotting in the liver tissues from a rat model of hepatic oxidative stress established by high-fat diet. We also assessed the level of reactive oxygen species (ROS) and mitochondrial membrane protential by fluorescence microscopy, tested glutathione (GSH) and superoxide dismutase (SOD) levels using ELISA, and detected mitochondrial DNA (mtDNA) content using real-time quantitative PCR in cultured hepatocytes (HHL-5 cells) under fatty acid-induced oxidative stress. Results In the rat model of hepatic oxidative stress, SFN significantly reduced high-fat diet-induced elevation of MDA level (P < 0.05) and up-regulated the protein expression of the antioxidant enzymes in the liver tissues (P < 0.05). In cultured HHL-5 cells, SFN obviously reduced free fatty acid-induced elevation of ROS level (P < 0.05) and increased GSH content (P < 0.05), SOD level (P < 0.05), mitochondrial membrane potential (P < 0.05), and the content of mtDNA (P < 0.05). Conclusion SFN can enhance the antioxidant capacity of hepatocytes to improve high-fat diet-induced hepatic oxidative stress in rats and provide protection of mitochondrial function in the hepatocytes.http://aammt.tmmu.edu.cn/Upload/rhtml/201908073.htmsulforaphanehigh-fat dietmitochondrial functionantioxidant capacity |
| spellingShingle | LEI Peng TIAN Sicong TENG Chunying SUN Yao SHAN Yujuan LI Baolong Effect of sulforaphane on antioxidant capacity and mitochondrial function of hepatocytes in vitro and in rats with hepatic oxidative stress Di-san junyi daxue xuebao sulforaphane high-fat diet mitochondrial function antioxidant capacity |
| title | Effect of sulforaphane on antioxidant capacity and mitochondrial function of hepatocytes in vitro and in rats with hepatic oxidative stress |
| title_full | Effect of sulforaphane on antioxidant capacity and mitochondrial function of hepatocytes in vitro and in rats with hepatic oxidative stress |
| title_fullStr | Effect of sulforaphane on antioxidant capacity and mitochondrial function of hepatocytes in vitro and in rats with hepatic oxidative stress |
| title_full_unstemmed | Effect of sulforaphane on antioxidant capacity and mitochondrial function of hepatocytes in vitro and in rats with hepatic oxidative stress |
| title_short | Effect of sulforaphane on antioxidant capacity and mitochondrial function of hepatocytes in vitro and in rats with hepatic oxidative stress |
| title_sort | effect of sulforaphane on antioxidant capacity and mitochondrial function of hepatocytes in vitro and in rats with hepatic oxidative stress |
| topic | sulforaphane high-fat diet mitochondrial function antioxidant capacity |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/201908073.htm |
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