Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro
Zinc oxide ZnO has been widely used in wound dressing, food additive and root canal filling material owing to its antibacterial and antifungal properties. This study describes potential cytotoxic effect of ZnO on human periodontal ligament fibroblasts hPDLFs in vitro. ZnO was characterized us...
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| Format: | Article |
| Language: | English |
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Hitit University
2018-03-01
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| Series: | Hittite Journal of Science and Engineering |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/1506754 |
| _version_ | 1797662630829096960 |
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| author | Sükran Seker |
| author_facet | Sükran Seker |
| author_sort | Sükran Seker |
| collection | DOAJ |
| description | Zinc oxide ZnO has been widely used in wound dressing, food additive and root canal filling material owing to its antibacterial and antifungal properties. This study describes potential cytotoxic effect of ZnO on human periodontal ligament fibroblasts hPDLFs in vitro. ZnO was characterized using BET Braun-Emmet-Teller and DLS dynamic light scattering analyses. The hPDLFs were treated with 0.1, 1, 10, 50 and 100 μg/mL of ZnO for 6 h, 24 h and 48 h durations. After exposure to ZnO, phase contrast microscopy, transmission electron microscopy TEM , mitochondrial function MTT and lactate dehydrogenase LDH assays were used to evaluate cell morphology, particle uptake, cell viability and membrane leakage. In addition, real time cell responses to ZnO exposure were monitored by impedance measurements. Results indicated that ZnO exposure caused toxic effect in a dose- and timedependent manner when compared to control group, especially at 50 and 100 μg/mL. |
| first_indexed | 2024-03-11T19:03:03Z |
| format | Article |
| id | doaj.art-14ef506730f8442a803def3e818e2c2e |
| institution | Directory Open Access Journal |
| issn | 2148-4171 |
| language | English |
| last_indexed | 2024-03-11T19:03:03Z |
| publishDate | 2018-03-01 |
| publisher | Hitit University |
| record_format | Article |
| series | Hittite Journal of Science and Engineering |
| spelling | doaj.art-14ef506730f8442a803def3e818e2c2e2023-10-10T11:17:28ZengHitit UniversityHittite Journal of Science and Engineering2148-41712018-03-01511510.17350/HJSE19030000070150Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In VitroSükran Seker0Ankara University Stem Cell Institute and Ankara University Faculty of Science, Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara, TURKEYZinc oxide ZnO has been widely used in wound dressing, food additive and root canal filling material owing to its antibacterial and antifungal properties. This study describes potential cytotoxic effect of ZnO on human periodontal ligament fibroblasts hPDLFs in vitro. ZnO was characterized using BET Braun-Emmet-Teller and DLS dynamic light scattering analyses. The hPDLFs were treated with 0.1, 1, 10, 50 and 100 μg/mL of ZnO for 6 h, 24 h and 48 h durations. After exposure to ZnO, phase contrast microscopy, transmission electron microscopy TEM , mitochondrial function MTT and lactate dehydrogenase LDH assays were used to evaluate cell morphology, particle uptake, cell viability and membrane leakage. In addition, real time cell responses to ZnO exposure were monitored by impedance measurements. Results indicated that ZnO exposure caused toxic effect in a dose- and timedependent manner when compared to control group, especially at 50 and 100 μg/mL.https://dergipark.org.tr/tr/download/article-file/1506754cytotoxicityzinc oxideperiodontal ligament fibroblastsimpedancein vitro toxicity assays |
| spellingShingle | Sükran Seker Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro Hittite Journal of Science and Engineering cytotoxicity zinc oxide periodontal ligament fibroblasts impedance in vitro toxicity assays |
| title | Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro |
| title_full | Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro |
| title_fullStr | Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro |
| title_full_unstemmed | Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro |
| title_short | Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro |
| title_sort | cytotoxic effects of zinc oxide on human periodontal ligament fibroblasts in vitro |
| topic | cytotoxicity zinc oxide periodontal ligament fibroblasts impedance in vitro toxicity assays |
| url | https://dergipark.org.tr/tr/download/article-file/1506754 |
| work_keys_str_mv | AT sukranseker cytotoxiceffectsofzincoxideonhumanperiodontalligamentfibroblastsinvitro |