Efficacy of Laser-Assisted Hatching on Pregnancy and Live-Birth Rates of Human Frozen-Thawed Embryos
Unexplained repeated in-vitro fertilization (IVF) failure is a major problem that faces both genders. The present study aims to estimate the influence of the laser-assisted hatching (LAH) technique on implantation, clinical pregnancy and live-birth rates using human cryopreserved–thawed embryos prod...
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| Format: | Article |
| Language: | English |
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Ain Shams University, Faculty of Women for Arts, Science & Education
2023-12-01
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| Series: | Journal of Scientific Research in Science |
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| Online Access: | https://jsrs.journals.ekb.eg/article_331811.html |
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| author | Shorouk El-Toukhy Mohamed Ali El-Desouky Ragaa Taha Mansour Mariam Abdur-Rahman |
| author_facet | Shorouk El-Toukhy Mohamed Ali El-Desouky Ragaa Taha Mansour Mariam Abdur-Rahman |
| author_sort | Shorouk El-Toukhy |
| collection | DOAJ |
| description | Unexplained repeated in-vitro fertilization (IVF) failure is a major problem that faces both genders. The present study aims to estimate the influence of the laser-assisted hatching (LAH) technique on implantation, clinical pregnancy and live-birth rates using human cryopreserved–thawed embryos produced by intracytoplasmic sperm injection (ICSI). Materials and methods: A total of 208 subjects was divided randomly into 100 subjects in the control group (Non-LAH), in which the uterine frozen embryo transfer (FET) was done directly; and 108 subjects in LAH group, in which laser shots were applied on day-3 frozen-thawed embryos before uterine transfer. All groups were further subdivided into three female age categories (22-30; 31-35 & 36-40 years). Number of mature, fertilized oocytes, fertilization, clinical pregnancy and live-birth rates were determined according to age distribution. Results: Both pregnancy (37.0 %) and live-birth (57.5 %) rates in LAH group were insignificantly lower than Non-LAH control group having pregnancy and live-birth rates of (45.0 % and 73.3 %, respectively). LAH caused a mild increase in clinical outcomes in females aged over 36 years, but in contrast, no improvement was observed in younger subjects. Conclusion: Our experimental study did not support using LAH as a routine strategy in frozen-thawed embryo transfer cycles. |
| first_indexed | 2024-03-08T05:16:43Z |
| format | Article |
| id | doaj.art-14f0c9f1cffd4c469f02a955944fb891 |
| institution | Directory Open Access Journal |
| issn | 2356-8364 2356-8372 |
| language | English |
| last_indexed | 2024-03-08T05:16:43Z |
| publishDate | 2023-12-01 |
| publisher | Ain Shams University, Faculty of Women for Arts, Science & Education |
| record_format | Article |
| series | Journal of Scientific Research in Science |
| spelling | doaj.art-14f0c9f1cffd4c469f02a955944fb8912024-02-06T16:19:04ZengAin Shams University, Faculty of Women for Arts, Science & EducationJournal of Scientific Research in Science2356-83642356-83722023-12-0140119821210.21608/JSRS.2023.331811Efficacy of Laser-Assisted Hatching on Pregnancy and Live-Birth Rates of Human Frozen-Thawed Embryos Shorouk El-Toukhy0Mohamed Ali El-Desouky1Ragaa Taha Mansour2Mariam Abdur-Rahman3Egyptian IVF-ET Center, Cairo, Egypt.Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt.Egyptian IVF-ET Center, Cairo, Egypt.Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt.Unexplained repeated in-vitro fertilization (IVF) failure is a major problem that faces both genders. The present study aims to estimate the influence of the laser-assisted hatching (LAH) technique on implantation, clinical pregnancy and live-birth rates using human cryopreserved–thawed embryos produced by intracytoplasmic sperm injection (ICSI). Materials and methods: A total of 208 subjects was divided randomly into 100 subjects in the control group (Non-LAH), in which the uterine frozen embryo transfer (FET) was done directly; and 108 subjects in LAH group, in which laser shots were applied on day-3 frozen-thawed embryos before uterine transfer. All groups were further subdivided into three female age categories (22-30; 31-35 & 36-40 years). Number of mature, fertilized oocytes, fertilization, clinical pregnancy and live-birth rates were determined according to age distribution. Results: Both pregnancy (37.0 %) and live-birth (57.5 %) rates in LAH group were insignificantly lower than Non-LAH control group having pregnancy and live-birth rates of (45.0 % and 73.3 %, respectively). LAH caused a mild increase in clinical outcomes in females aged over 36 years, but in contrast, no improvement was observed in younger subjects. Conclusion: Our experimental study did not support using LAH as a routine strategy in frozen-thawed embryo transfer cycles. https://jsrs.journals.ekb.eg/article_331811.htmlin-vitro fertilizationicsilaser-assisted hatchingfrozen embryospregnancy |
| spellingShingle | Shorouk El-Toukhy Mohamed Ali El-Desouky Ragaa Taha Mansour Mariam Abdur-Rahman Efficacy of Laser-Assisted Hatching on Pregnancy and Live-Birth Rates of Human Frozen-Thawed Embryos Journal of Scientific Research in Science in-vitro fertilization icsi laser-assisted hatching frozen embryos pregnancy |
| title | Efficacy of Laser-Assisted Hatching on Pregnancy and Live-Birth Rates of Human Frozen-Thawed Embryos |
| title_full | Efficacy of Laser-Assisted Hatching on Pregnancy and Live-Birth Rates of Human Frozen-Thawed Embryos |
| title_fullStr | Efficacy of Laser-Assisted Hatching on Pregnancy and Live-Birth Rates of Human Frozen-Thawed Embryos |
| title_full_unstemmed | Efficacy of Laser-Assisted Hatching on Pregnancy and Live-Birth Rates of Human Frozen-Thawed Embryos |
| title_short | Efficacy of Laser-Assisted Hatching on Pregnancy and Live-Birth Rates of Human Frozen-Thawed Embryos |
| title_sort | efficacy of laser assisted hatching on pregnancy and live birth rates of human frozen thawed embryos |
| topic | in-vitro fertilization icsi laser-assisted hatching frozen embryos pregnancy |
| url | https://jsrs.journals.ekb.eg/article_331811.html |
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