Combination of Host-Associated <i>Rummeliibacillus</i> sp. and <i>Microbacterium</i> sp. Positively Modulated the Growth, Feed Utilization, and Intestinal Microbial Population of Olive Flounder (<i>Paralichthys olivaceus</i>)
Two novel strains of <i>Rummeliibacillus</i> sp. and <i>Microbacterium</i> sp. were identified from the intestine of olive flounder (<i>Paralichthys olivaceus</i>) and characterized in vitro as potential probiotics. Feeds without probiotic and with a 50:50 mixture...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/12/11/1443 |
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| author | Su-Jeong Lee So Hee Kim Da-In Noh Young-Sun Lee Tae-Rim Kim Md Tawheed Hasan Eun-Woo Lee Won Je Jang |
| author_facet | Su-Jeong Lee So Hee Kim Da-In Noh Young-Sun Lee Tae-Rim Kim Md Tawheed Hasan Eun-Woo Lee Won Je Jang |
| author_sort | Su-Jeong Lee |
| collection | DOAJ |
| description | Two novel strains of <i>Rummeliibacillus</i> sp. and <i>Microbacterium</i> sp. were identified from the intestine of olive flounder (<i>Paralichthys olivaceus</i>) and characterized in vitro as potential probiotics. Feeds without probiotic and with a 50:50 mixture of these two strains (1 × 10<sup>8</sup> CFU/g feed) were denoted as the control and Pro diets, respectively. Three randomly selected tanks (20 flounders/tank, ~11.4 g each) were used for each diet replication. After 8 weeks of feeding, the growth and feed utilization of the flounder in the Pro group improved (<i>p</i> < 0.05) compared to the control. Among four immune parameters, only myeloperoxidase activity was elevated in the Pro group. Serum biochemistry, intestinal microbial richness (Chao1), and diversity (Shannon index) remained unchanged (<i>p</i> ≥ 0.05), but phylogenetic diversity was enriched in the Pro fish intestine. Significantly lower Firmicutes and higher Proteobacteria were found in the Pro diet; the genus abundance in the control and Pro was as follows: <i>Staphylococcus</i> > <i>Lactobacillus</i> > <i>Corynebacterium</i> and <i>Lactobacillus</i> > <i>Staphylococcus</i> > <i>Corynebacterium</i>, respectively. Microbial linear discriminant scores and a cladogram analysis showed significant modulation. Therefore, the combination of two host-associated probiotics improved the growth and intestinal microbial population of flounder and could be supplemented in the Korean flounder industry. |
| first_indexed | 2024-03-09T17:00:14Z |
| format | Article |
| id | doaj.art-14f116b0ef724460b604df07d413b65b |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T17:00:14Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-14f116b0ef724460b604df07d413b65b2023-11-24T14:30:29ZengMDPI AGBiology2079-77372023-11-011211144310.3390/biology12111443Combination of Host-Associated <i>Rummeliibacillus</i> sp. and <i>Microbacterium</i> sp. Positively Modulated the Growth, Feed Utilization, and Intestinal Microbial Population of Olive Flounder (<i>Paralichthys olivaceus</i>)Su-Jeong Lee0So Hee Kim1Da-In Noh2Young-Sun Lee3Tae-Rim Kim4Md Tawheed Hasan5Eun-Woo Lee6Won Je Jang7Biopharmaceutical Engineering Major, Dong-Eui University, Busan 47340, Republic of KoreaSoutheast Sea Fisheries Research Institute, National Institute of Fisheries Science, Tongyeong 53085, Republic of KoreaBiopharmaceutical Engineering Major, Dong-Eui University, Busan 47340, Republic of KoreaBiopharmaceutical Engineering Major, Dong-Eui University, Busan 47340, Republic of KoreaBiopharmaceutical Engineering Major, Dong-Eui University, Busan 47340, Republic of KoreaDepartment of Aquaculture, Sylhet Agricultural University, Sylhet 3100, BangladeshBiopharmaceutical Engineering Major, Dong-Eui University, Busan 47340, Republic of KoreaBiopharmaceutical Engineering Major, Dong-Eui University, Busan 47340, Republic of KoreaTwo novel strains of <i>Rummeliibacillus</i> sp. and <i>Microbacterium</i> sp. were identified from the intestine of olive flounder (<i>Paralichthys olivaceus</i>) and characterized in vitro as potential probiotics. Feeds without probiotic and with a 50:50 mixture of these two strains (1 × 10<sup>8</sup> CFU/g feed) were denoted as the control and Pro diets, respectively. Three randomly selected tanks (20 flounders/tank, ~11.4 g each) were used for each diet replication. After 8 weeks of feeding, the growth and feed utilization of the flounder in the Pro group improved (<i>p</i> < 0.05) compared to the control. Among four immune parameters, only myeloperoxidase activity was elevated in the Pro group. Serum biochemistry, intestinal microbial richness (Chao1), and diversity (Shannon index) remained unchanged (<i>p</i> ≥ 0.05), but phylogenetic diversity was enriched in the Pro fish intestine. Significantly lower Firmicutes and higher Proteobacteria were found in the Pro diet; the genus abundance in the control and Pro was as follows: <i>Staphylococcus</i> > <i>Lactobacillus</i> > <i>Corynebacterium</i> and <i>Lactobacillus</i> > <i>Staphylococcus</i> > <i>Corynebacterium</i>, respectively. Microbial linear discriminant scores and a cladogram analysis showed significant modulation. Therefore, the combination of two host-associated probiotics improved the growth and intestinal microbial population of flounder and could be supplemented in the Korean flounder industry.https://www.mdpi.com/2079-7737/12/11/1443<i>Rummeliibacillus</i> sp.<i>Microbacterium</i> sp.olive flounderprobioticsmicrobiota |
| spellingShingle | Su-Jeong Lee So Hee Kim Da-In Noh Young-Sun Lee Tae-Rim Kim Md Tawheed Hasan Eun-Woo Lee Won Je Jang Combination of Host-Associated <i>Rummeliibacillus</i> sp. and <i>Microbacterium</i> sp. Positively Modulated the Growth, Feed Utilization, and Intestinal Microbial Population of Olive Flounder (<i>Paralichthys olivaceus</i>) Biology <i>Rummeliibacillus</i> sp. <i>Microbacterium</i> sp. olive flounder probiotics microbiota |
| title | Combination of Host-Associated <i>Rummeliibacillus</i> sp. and <i>Microbacterium</i> sp. Positively Modulated the Growth, Feed Utilization, and Intestinal Microbial Population of Olive Flounder (<i>Paralichthys olivaceus</i>) |
| title_full | Combination of Host-Associated <i>Rummeliibacillus</i> sp. and <i>Microbacterium</i> sp. Positively Modulated the Growth, Feed Utilization, and Intestinal Microbial Population of Olive Flounder (<i>Paralichthys olivaceus</i>) |
| title_fullStr | Combination of Host-Associated <i>Rummeliibacillus</i> sp. and <i>Microbacterium</i> sp. Positively Modulated the Growth, Feed Utilization, and Intestinal Microbial Population of Olive Flounder (<i>Paralichthys olivaceus</i>) |
| title_full_unstemmed | Combination of Host-Associated <i>Rummeliibacillus</i> sp. and <i>Microbacterium</i> sp. Positively Modulated the Growth, Feed Utilization, and Intestinal Microbial Population of Olive Flounder (<i>Paralichthys olivaceus</i>) |
| title_short | Combination of Host-Associated <i>Rummeliibacillus</i> sp. and <i>Microbacterium</i> sp. Positively Modulated the Growth, Feed Utilization, and Intestinal Microbial Population of Olive Flounder (<i>Paralichthys olivaceus</i>) |
| title_sort | combination of host associated i rummeliibacillus i sp and i microbacterium i sp positively modulated the growth feed utilization and intestinal microbial population of olive flounder i paralichthys olivaceus i |
| topic | <i>Rummeliibacillus</i> sp. <i>Microbacterium</i> sp. olive flounder probiotics microbiota |
| url | https://www.mdpi.com/2079-7737/12/11/1443 |
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