Recent Research Progress in Taxol Biosynthetic Pathway and Acylation Reactions Mediated by <i>Taxus</i> Acyltransferases
Taxol is one of the most effective anticancer drugs in the world that is widely used in the treatments of breast, lung and ovarian cancer. The elucidation of the taxol biosynthetic pathway is the key to solve the problem of taxol supply. So far, the taxol biosynthetic pathway has been reported to re...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/10/2855 |
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| author | Tao Wang Lingyu Li Weibing Zhuang Fengjiao Zhang Xiaochun Shu Ning Wang Zhong Wang |
| author_facet | Tao Wang Lingyu Li Weibing Zhuang Fengjiao Zhang Xiaochun Shu Ning Wang Zhong Wang |
| author_sort | Tao Wang |
| collection | DOAJ |
| description | Taxol is one of the most effective anticancer drugs in the world that is widely used in the treatments of breast, lung and ovarian cancer. The elucidation of the taxol biosynthetic pathway is the key to solve the problem of taxol supply. So far, the taxol biosynthetic pathway has been reported to require an estimated 20 steps of enzymatic reactions, and sixteen enzymes involved in the taxol pathway have been well characterized, including a novel taxane-10β-hydroxylase (T10βOH) and a newly putative β-phenylalanyl-CoA ligase (PCL). Moreover, the source and formation of the taxane core and the details of the downstream synthetic pathway have been basically depicted, while the modification of the core taxane skeleton has not been fully reported, mainly concerning the developments from diol intermediates to 2-debenzoyltaxane. The acylation reaction mediated by specialized <i>Taxus</i> BAHD family acyltransferases (ACTs) is recognized as one of the most important steps in the modification of core taxane skeleton that contribute to the increase of taxol yield. Recently, the influence of acylation on the functional and structural diversity of taxanes has also been continuously revealed. This review summarizes the latest research advances of the taxol biosynthetic pathway and systematically discusses the acylation reactions supported by <i>Taxus</i> ACTs. The underlying mechanism could improve the understanding of taxol biosynthesis, and provide a theoretical basis for the mass production of taxol. |
| first_indexed | 2024-03-10T11:30:22Z |
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| id | doaj.art-14f2c6f2070e4896a55ac22c9f08c86c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T11:30:22Z |
| publishDate | 2021-05-01 |
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| spelling | doaj.art-14f2c6f2070e4896a55ac22c9f08c86c2023-11-21T19:15:52ZengMDPI AGMolecules1420-30492021-05-012610285510.3390/molecules26102855Recent Research Progress in Taxol Biosynthetic Pathway and Acylation Reactions Mediated by <i>Taxus</i> AcyltransferasesTao Wang0Lingyu Li1Weibing Zhuang2Fengjiao Zhang3Xiaochun Shu4Ning Wang5Zhong Wang6Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaTaxol is one of the most effective anticancer drugs in the world that is widely used in the treatments of breast, lung and ovarian cancer. The elucidation of the taxol biosynthetic pathway is the key to solve the problem of taxol supply. So far, the taxol biosynthetic pathway has been reported to require an estimated 20 steps of enzymatic reactions, and sixteen enzymes involved in the taxol pathway have been well characterized, including a novel taxane-10β-hydroxylase (T10βOH) and a newly putative β-phenylalanyl-CoA ligase (PCL). Moreover, the source and formation of the taxane core and the details of the downstream synthetic pathway have been basically depicted, while the modification of the core taxane skeleton has not been fully reported, mainly concerning the developments from diol intermediates to 2-debenzoyltaxane. The acylation reaction mediated by specialized <i>Taxus</i> BAHD family acyltransferases (ACTs) is recognized as one of the most important steps in the modification of core taxane skeleton that contribute to the increase of taxol yield. Recently, the influence of acylation on the functional and structural diversity of taxanes has also been continuously revealed. This review summarizes the latest research advances of the taxol biosynthetic pathway and systematically discusses the acylation reactions supported by <i>Taxus</i> ACTs. The underlying mechanism could improve the understanding of taxol biosynthesis, and provide a theoretical basis for the mass production of taxol.https://www.mdpi.com/1420-3049/26/10/2855taxolbiosynthetic pathwayacylation reactionacyltransferaselatest research progress |
| spellingShingle | Tao Wang Lingyu Li Weibing Zhuang Fengjiao Zhang Xiaochun Shu Ning Wang Zhong Wang Recent Research Progress in Taxol Biosynthetic Pathway and Acylation Reactions Mediated by <i>Taxus</i> Acyltransferases Molecules taxol biosynthetic pathway acylation reaction acyltransferase latest research progress |
| title | Recent Research Progress in Taxol Biosynthetic Pathway and Acylation Reactions Mediated by <i>Taxus</i> Acyltransferases |
| title_full | Recent Research Progress in Taxol Biosynthetic Pathway and Acylation Reactions Mediated by <i>Taxus</i> Acyltransferases |
| title_fullStr | Recent Research Progress in Taxol Biosynthetic Pathway and Acylation Reactions Mediated by <i>Taxus</i> Acyltransferases |
| title_full_unstemmed | Recent Research Progress in Taxol Biosynthetic Pathway and Acylation Reactions Mediated by <i>Taxus</i> Acyltransferases |
| title_short | Recent Research Progress in Taxol Biosynthetic Pathway and Acylation Reactions Mediated by <i>Taxus</i> Acyltransferases |
| title_sort | recent research progress in taxol biosynthetic pathway and acylation reactions mediated by i taxus i acyltransferases |
| topic | taxol biosynthetic pathway acylation reaction acyltransferase latest research progress |
| url | https://www.mdpi.com/1420-3049/26/10/2855 |
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