Irradiation-induced exosomal HMGB1 to confer radioresistance via the PI3K/AKT/FOXO3A signaling pathway in ESCC
Abstract Background Radioresistance is a major cause of treatment failure in esophageal squamous cell carcinoma (ESCC) radiotherapy, and the underlying mechanisms of radioresistance are still unclear. Irradiation (IR) stimulates changes in tumor-derived exosome contents, which can be taken up by rec...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12967-022-03720-0 |
| _version_ | 1797984656850681856 |
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| author | Xingyu Du Xueyuan Zhang Jing Dong Naiyi Zou Dong Guo Weinan Yao Xiaobin Wang Shuguang Li Chunyang Song Ke Yan Wenbin Shen Shuchai Zhu |
| author_facet | Xingyu Du Xueyuan Zhang Jing Dong Naiyi Zou Dong Guo Weinan Yao Xiaobin Wang Shuguang Li Chunyang Song Ke Yan Wenbin Shen Shuchai Zhu |
| author_sort | Xingyu Du |
| collection | DOAJ |
| description | Abstract Background Radioresistance is a major cause of treatment failure in esophageal squamous cell carcinoma (ESCC) radiotherapy, and the underlying mechanisms of radioresistance are still unclear. Irradiation (IR) stimulates changes in tumor-derived exosome contents, which can be taken up by recipient cells, playing an important role in the proliferation, cell cycle and apoptosis of recipient cells. This study investigated the effect of IR-induced exosomal high mobility group box 1 (HMGB1) on radioresistance in ESCC cells. Methods Plasma exosomes were isolated from 21 ESCC patients and 24 healthy volunteers, and the expression of HMGB1 was examined. Then, the therapeutic effect of radiotherapy was analyzed according to the different expression levels of plasma exosomal HMGB1 in ESCC patients. The uptake of exosomes by recipient cells was verified by immunofluorescence staining, and the localization of exosomes and HMGB1 in cells before and after IR was evaluated. The effects of IR-induced exosomes on cell proliferation, invasion, apoptosis, cell cycle distribution and radioresistance after HMGB1 knockdown were verified. Moreover, western blotting was used to measure changes in the expression of cyclin B1, CDK1, Bax, Bcl2, phosphorylated histone H2AX and the PI3K/AKT/FOXO3A pathway in the HMGB1-knockdown exosome group and the negative control group. Results The expression of HMGB1 in ESCC plasma exosomes was significantly increased compared with that in healthy volunteers, and high expression of HMGB1 in plasma exosomes was associated with radioresistance (P = 0.016). IR-induced the release of exosomal HMGB1 and promoted proliferation and radioresistance in recipient cells, with a sensitization enhancement ratio (SER) of 0.906 and 0.919, respectively. In addition, IR-induced exosomal HMGB1 promotes G2/M phase arrest by regulating the proteins cyclin B1 and CDK1, cooperating with the proteins Bax and Bcl2 to reduce the apoptosis rate through the PI3K/AKT/FOXO3A signaling pathway, and participated in IR-induced DNA damage repair through γH2AX. Conclusion These findings indicate that high expression of plasma exosomal HMGB1 is associated with an adverse radiotherapy response. IR-induced exosomal HMGB1 enhances the radioresistance of ESCC cells. |
| first_indexed | 2024-04-11T07:06:26Z |
| format | Article |
| id | doaj.art-14f2e25f3cb141f7af2a16e6566e0896 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-04-11T07:06:26Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-14f2e25f3cb141f7af2a16e6566e08962022-12-22T04:38:23ZengBMCJournal of Translational Medicine1479-58762022-11-0120111510.1186/s12967-022-03720-0Irradiation-induced exosomal HMGB1 to confer radioresistance via the PI3K/AKT/FOXO3A signaling pathway in ESCCXingyu Du0Xueyuan Zhang1Jing Dong2Naiyi Zou3Dong Guo4Weinan Yao5Xiaobin Wang6Shuguang Li7Chunyang Song8Ke Yan9Wenbin Shen10Shuchai Zhu11Department of Radiotherapy, The Fourth Hospital of Hebei Medical UniversityDepartment of Radiotherapy, The Fourth Hospital of Hebei Medical UniversityDepartment of Radiotherapy, The Fourth Hospital of Hebei Medical UniversityDepartment of Radiotherapy, The Fourth Hospital of Hebei Medical UniversityDepartment of Radiotherapy, The Fourth Hospital of Hebei Medical UniversityDepartment of Radiotherapy, The Fourth Hospital of Hebei Medical UniversityDepartment of Radiotherapy, The Fourth Hospital of Hebei Medical UniversityDepartment of Radiotherapy, The Fourth Hospital of Hebei Medical UniversityDepartment of Radiotherapy, The Fourth Hospital of Hebei Medical UniversityDepartment of Radiotherapy, The Fourth Hospital of Hebei Medical UniversityDepartment of Radiotherapy, The Fourth Hospital of Hebei Medical UniversityDepartment of Radiotherapy, The Fourth Hospital of Hebei Medical UniversityAbstract Background Radioresistance is a major cause of treatment failure in esophageal squamous cell carcinoma (ESCC) radiotherapy, and the underlying mechanisms of radioresistance are still unclear. Irradiation (IR) stimulates changes in tumor-derived exosome contents, which can be taken up by recipient cells, playing an important role in the proliferation, cell cycle and apoptosis of recipient cells. This study investigated the effect of IR-induced exosomal high mobility group box 1 (HMGB1) on radioresistance in ESCC cells. Methods Plasma exosomes were isolated from 21 ESCC patients and 24 healthy volunteers, and the expression of HMGB1 was examined. Then, the therapeutic effect of radiotherapy was analyzed according to the different expression levels of plasma exosomal HMGB1 in ESCC patients. The uptake of exosomes by recipient cells was verified by immunofluorescence staining, and the localization of exosomes and HMGB1 in cells before and after IR was evaluated. The effects of IR-induced exosomes on cell proliferation, invasion, apoptosis, cell cycle distribution and radioresistance after HMGB1 knockdown were verified. Moreover, western blotting was used to measure changes in the expression of cyclin B1, CDK1, Bax, Bcl2, phosphorylated histone H2AX and the PI3K/AKT/FOXO3A pathway in the HMGB1-knockdown exosome group and the negative control group. Results The expression of HMGB1 in ESCC plasma exosomes was significantly increased compared with that in healthy volunteers, and high expression of HMGB1 in plasma exosomes was associated with radioresistance (P = 0.016). IR-induced the release of exosomal HMGB1 and promoted proliferation and radioresistance in recipient cells, with a sensitization enhancement ratio (SER) of 0.906 and 0.919, respectively. In addition, IR-induced exosomal HMGB1 promotes G2/M phase arrest by regulating the proteins cyclin B1 and CDK1, cooperating with the proteins Bax and Bcl2 to reduce the apoptosis rate through the PI3K/AKT/FOXO3A signaling pathway, and participated in IR-induced DNA damage repair through γH2AX. Conclusion These findings indicate that high expression of plasma exosomal HMGB1 is associated with an adverse radiotherapy response. IR-induced exosomal HMGB1 enhances the radioresistance of ESCC cells.https://doi.org/10.1186/s12967-022-03720-0ExosomesHMGB1RadioresistanceESCC |
| spellingShingle | Xingyu Du Xueyuan Zhang Jing Dong Naiyi Zou Dong Guo Weinan Yao Xiaobin Wang Shuguang Li Chunyang Song Ke Yan Wenbin Shen Shuchai Zhu Irradiation-induced exosomal HMGB1 to confer radioresistance via the PI3K/AKT/FOXO3A signaling pathway in ESCC Journal of Translational Medicine Exosomes HMGB1 Radioresistance ESCC |
| title | Irradiation-induced exosomal HMGB1 to confer radioresistance via the PI3K/AKT/FOXO3A signaling pathway in ESCC |
| title_full | Irradiation-induced exosomal HMGB1 to confer radioresistance via the PI3K/AKT/FOXO3A signaling pathway in ESCC |
| title_fullStr | Irradiation-induced exosomal HMGB1 to confer radioresistance via the PI3K/AKT/FOXO3A signaling pathway in ESCC |
| title_full_unstemmed | Irradiation-induced exosomal HMGB1 to confer radioresistance via the PI3K/AKT/FOXO3A signaling pathway in ESCC |
| title_short | Irradiation-induced exosomal HMGB1 to confer radioresistance via the PI3K/AKT/FOXO3A signaling pathway in ESCC |
| title_sort | irradiation induced exosomal hmgb1 to confer radioresistance via the pi3k akt foxo3a signaling pathway in escc |
| topic | Exosomes HMGB1 Radioresistance ESCC |
| url | https://doi.org/10.1186/s12967-022-03720-0 |
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