Over-Expression of βII-Tubulin and Especially Its Localization in Cell Nuclei Correlates with Poorer Outcomes in Colorectal Cancer
Tubulin is a heterodimer of α and β subunits, both existing as isotypes differing in amino acid sequence encoded by different genes. Specific isotypes of tubulin have associations with cancer that are not well understood. Previous studies found that βII-tubulin is express...
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MDPI AG
2019-01-01
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| Series: | Cells |
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| Online Access: | http://www.mdpi.com/2073-4409/8/1/25 |
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| author | Kseniya Ruksha Artur Mezheyeuski Alexander Nerovnya Tatyana Bich Gennady Tur Julia Gorgun Richard Luduena Anna Portyanko |
| author_facet | Kseniya Ruksha Artur Mezheyeuski Alexander Nerovnya Tatyana Bich Gennady Tur Julia Gorgun Richard Luduena Anna Portyanko |
| author_sort | Kseniya Ruksha |
| collection | DOAJ |
| description | Tubulin is a heterodimer of α and β subunits, both existing as isotypes differing in amino acid sequence encoded by different genes. Specific isotypes of tubulin have associations with cancer that are not well understood. Previous studies found that βII-tubulin is expressed in a number of transformed cells and that this isotype is found in cell nuclei in non-microtubule form. The association of βII expression and its nuclear localization with cancer progression has not previously been addressed. We here used a monoclonal antibody to βII to examine patients with colorectal cancer and found that patients whose tumors over-express βII have a greatly decreased life expectancy which is even shorter in those patients with nuclear βII. Our results suggest that βII-tubulin may facilitate cancer growth and metastasis and, to accomplish this, may not need to be in microtubule form. Furthermore, βII expression and localization could be a useful prognostic marker. We also found that βII appears in the nuclei of otherwise normal cells adjacent to the tumor. It is possible therefore that cancer cells expressing βII influence nearby cells to do the same and to localize βII in their nuclei by an as yet uncharacterized regulatory pathway. |
| first_indexed | 2024-03-12T06:42:54Z |
| format | Article |
| id | doaj.art-14f60416ad664219a8d6a18d45b813a0 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T06:42:54Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-14f60416ad664219a8d6a18d45b813a02023-09-03T00:50:30ZengMDPI AGCells2073-44092019-01-01812510.3390/cells8010025cells8010025Over-Expression of βII-Tubulin and Especially Its Localization in Cell Nuclei Correlates with Poorer Outcomes in Colorectal CancerKseniya Ruksha0Artur Mezheyeuski1Alexander Nerovnya2Tatyana Bich3Gennady Tur4Julia Gorgun5Richard Luduena6Anna Portyanko7N.N. Alexandrov National Cancer Centre of Belarus, 223040 Minsk, BelarusDepartment of Pathology, Belarusian State Medical University, 220116 Minsk, BelarusDepartment of Pathology, Belarusian State Medical University, 220116 Minsk, BelarusDepartment of Pathology, Belarusian State Medical University, 220116 Minsk, BelarusMinsk City Clinical Oncologic Dispensary, 220013 Minsk, BelarusDepartment of Gastroenterology and Nutrition, Belarusian Medical Academy of Post-Graduate Education, 220013 Minsk, BelarusDepartment of Biochemistry and Structural Biology, University of Texas Health San Antonio, San Antonio, TX 78229, USADepartment of Pathology, Belarusian State Medical University, 220116 Minsk, BelarusTubulin is a heterodimer of α and β subunits, both existing as isotypes differing in amino acid sequence encoded by different genes. Specific isotypes of tubulin have associations with cancer that are not well understood. Previous studies found that βII-tubulin is expressed in a number of transformed cells and that this isotype is found in cell nuclei in non-microtubule form. The association of βII expression and its nuclear localization with cancer progression has not previously been addressed. We here used a monoclonal antibody to βII to examine patients with colorectal cancer and found that patients whose tumors over-express βII have a greatly decreased life expectancy which is even shorter in those patients with nuclear βII. Our results suggest that βII-tubulin may facilitate cancer growth and metastasis and, to accomplish this, may not need to be in microtubule form. Furthermore, βII expression and localization could be a useful prognostic marker. We also found that βII appears in the nuclei of otherwise normal cells adjacent to the tumor. It is possible therefore that cancer cells expressing βII influence nearby cells to do the same and to localize βII in their nuclei by an as yet uncharacterized regulatory pathway.http://www.mdpi.com/2073-4409/8/1/25βII-tubulinmicrotubulesnuclear tubulincolorectal cancercancer prognosis |
| spellingShingle | Kseniya Ruksha Artur Mezheyeuski Alexander Nerovnya Tatyana Bich Gennady Tur Julia Gorgun Richard Luduena Anna Portyanko Over-Expression of βII-Tubulin and Especially Its Localization in Cell Nuclei Correlates with Poorer Outcomes in Colorectal Cancer Cells βII-tubulin microtubules nuclear tubulin colorectal cancer cancer prognosis |
| title | Over-Expression of βII-Tubulin and Especially Its Localization in Cell Nuclei Correlates with Poorer Outcomes in Colorectal Cancer |
| title_full | Over-Expression of βII-Tubulin and Especially Its Localization in Cell Nuclei Correlates with Poorer Outcomes in Colorectal Cancer |
| title_fullStr | Over-Expression of βII-Tubulin and Especially Its Localization in Cell Nuclei Correlates with Poorer Outcomes in Colorectal Cancer |
| title_full_unstemmed | Over-Expression of βII-Tubulin and Especially Its Localization in Cell Nuclei Correlates with Poorer Outcomes in Colorectal Cancer |
| title_short | Over-Expression of βII-Tubulin and Especially Its Localization in Cell Nuclei Correlates with Poorer Outcomes in Colorectal Cancer |
| title_sort | over expression of βii tubulin and especially its localization in cell nuclei correlates with poorer outcomes in colorectal cancer |
| topic | βII-tubulin microtubules nuclear tubulin colorectal cancer cancer prognosis |
| url | http://www.mdpi.com/2073-4409/8/1/25 |
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