MTBP promoted the proliferation, migration and invasion of colon cancer cells by activating the expression of ZEB2
Colon cancer is a malignant tumor that seriously affects human health. Recently, studies revealed that the expression of MTBP enhanced the proliferation and metastasis of many types of cancer cells. And the data also showed that MTBP has the potential to regulate the expression of ZEB2. However, it...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-05-01
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| Series: | Animal Cells and Systems |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19768354.2021.1938218 |
| _version_ | 1818656319412895744 |
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| author | Paerhati Shayimu Aikeremu Yusufu Aizimaiti Rehemutula Darebai Redati Rexida Jiapaer Rousidan Tuerdi |
| author_facet | Paerhati Shayimu Aikeremu Yusufu Aizimaiti Rehemutula Darebai Redati Rexida Jiapaer Rousidan Tuerdi |
| author_sort | Paerhati Shayimu |
| collection | DOAJ |
| description | Colon cancer is a malignant tumor that seriously affects human health. Recently, studies revealed that the expression of MTBP enhanced the proliferation and metastasis of many types of cancer cells. And the data also showed that MTBP has the potential to regulate the expression of ZEB2. However, it is unclear whether MTBP can affect the proliferation, migration and invasion of colon cancer cells by modulating the expression of ZEB2. In this study, we established the MTBP overexpression and knockdown colon cancer cells with the transfection. Next, CCK-8 and transwell assays were carried out to determine the changes of the proliferation and invasion of colon cancer cells, respectively. After that, we overexpressed the ZEB2 in these MTBP knockdown colon cancer cells. Finally, the invasion and migration of these cells were detected with the same methods. We revealed that overexpression of MTBP enhanced the proliferation and invasion of colon cancer cells. Moreover, suppression of MTBP repressed the proliferation, migration and invasion of colon cancer cells. Furthermore, MTBP promoted the expression of ZEB2. The overexpression of ZEB2 abolished the MTBP knockdown induced inhibition of the migration and invasion of colon cancer cells. These results implied that MTBP enhanced the proliferation, migration and invasion of colon cancer cells by activating the expression of ZEB2. |
| first_indexed | 2024-12-17T03:23:42Z |
| format | Article |
| id | doaj.art-14fa8d485ebb42959523360e5ed94936 |
| institution | Directory Open Access Journal |
| issn | 1976-8354 2151-2485 |
| language | English |
| last_indexed | 2024-12-17T03:23:42Z |
| publishDate | 2021-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj.art-14fa8d485ebb42959523360e5ed949362022-12-21T22:05:26ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852021-05-0125315216010.1080/19768354.2021.19382181938218MTBP promoted the proliferation, migration and invasion of colon cancer cells by activating the expression of ZEB2Paerhati Shayimu0Aikeremu Yusufu1Aizimaiti Rehemutula2Darebai Redati3Rexida Jiapaer4Rousidan Tuerdi5Cancer Hospital of Xinjiang Medical UniversityCancer Hospital of Xinjiang Medical UniversityCancer Hospital of Xinjiang Medical UniversityB-Ultrasound Room, Cancer Hospital of Xinjiang Medical UniversityCancer Hospital of Xinjiang Medical UniversityXinjiang Medical UniversityColon cancer is a malignant tumor that seriously affects human health. Recently, studies revealed that the expression of MTBP enhanced the proliferation and metastasis of many types of cancer cells. And the data also showed that MTBP has the potential to regulate the expression of ZEB2. However, it is unclear whether MTBP can affect the proliferation, migration and invasion of colon cancer cells by modulating the expression of ZEB2. In this study, we established the MTBP overexpression and knockdown colon cancer cells with the transfection. Next, CCK-8 and transwell assays were carried out to determine the changes of the proliferation and invasion of colon cancer cells, respectively. After that, we overexpressed the ZEB2 in these MTBP knockdown colon cancer cells. Finally, the invasion and migration of these cells were detected with the same methods. We revealed that overexpression of MTBP enhanced the proliferation and invasion of colon cancer cells. Moreover, suppression of MTBP repressed the proliferation, migration and invasion of colon cancer cells. Furthermore, MTBP promoted the expression of ZEB2. The overexpression of ZEB2 abolished the MTBP knockdown induced inhibition of the migration and invasion of colon cancer cells. These results implied that MTBP enhanced the proliferation, migration and invasion of colon cancer cells by activating the expression of ZEB2.http://dx.doi.org/10.1080/19768354.2021.1938218colon cancer cellsmtbpzeb2proliferationinvasion |
| spellingShingle | Paerhati Shayimu Aikeremu Yusufu Aizimaiti Rehemutula Darebai Redati Rexida Jiapaer Rousidan Tuerdi MTBP promoted the proliferation, migration and invasion of colon cancer cells by activating the expression of ZEB2 Animal Cells and Systems colon cancer cells mtbp zeb2 proliferation invasion |
| title | MTBP promoted the proliferation, migration and invasion of colon cancer cells by activating the expression of ZEB2 |
| title_full | MTBP promoted the proliferation, migration and invasion of colon cancer cells by activating the expression of ZEB2 |
| title_fullStr | MTBP promoted the proliferation, migration and invasion of colon cancer cells by activating the expression of ZEB2 |
| title_full_unstemmed | MTBP promoted the proliferation, migration and invasion of colon cancer cells by activating the expression of ZEB2 |
| title_short | MTBP promoted the proliferation, migration and invasion of colon cancer cells by activating the expression of ZEB2 |
| title_sort | mtbp promoted the proliferation migration and invasion of colon cancer cells by activating the expression of zeb2 |
| topic | colon cancer cells mtbp zeb2 proliferation invasion |
| url | http://dx.doi.org/10.1080/19768354.2021.1938218 |
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