ABCA1 gene promoter methylation and sudden cardiac death
Aim. A study of the association of the methylation of the promoter of the ABCA1 gene with sudden cardiac death(SCD).Materials and methods. The study design is based on the case-control principle. The SCD group included 150 men (mean age (46.7 ± 9.2) years) who died of sudden cardiac death according...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Siberian State Medical University (Tomsk)
2021-01-01
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| Series: | Бюллетень сибирской медицины |
| Subjects: | |
| Online Access: | https://bulletin.ssmu.ru/jour/article/view/4153 |
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| author | A. A. Ivanova A. A. Gurazheva E. I. Akinshina S. V. Maksimova S. K. Malyutina V. P. Novoselov I. A. Rodina O. V. Khamovich V. N. Maksimov |
| author_facet | A. A. Ivanova A. A. Gurazheva E. I. Akinshina S. V. Maksimova S. K. Malyutina V. P. Novoselov I. A. Rodina O. V. Khamovich V. N. Maksimov |
| author_sort | A. A. Ivanova |
| collection | DOAJ |
| description | Aim. A study of the association of the methylation of the promoter of the ABCA1 gene with sudden cardiac death(SCD).Materials and methods. The study design is based on the case-control principle. The SCD group included 150 men (mean age (46.7 ± 9.2) years) who died of sudden cardiac death according to forensic medical examination data (the main pathological diagnoses are acute circulatory failure, acute coronary insufficiency). The control group included 150 men (mean age (42.6 ± 1.2) years) who died suddenly, but not due to cardiovascular pathology. DNA was isolated by phenol-chloroform extraction from myocardial tissue. The methylation status of the ABCA1 gene promoter was assessed by methyl-specific polymerase chain reaction. The results obtained were statistically processed in SPSS 16.0 using Pearson’s test and Fisher’s test with Yates’ correction for continuity. P < 0.05 was used as a level of significance.Results. Comparing the groups revealed statistically significant differences in the methylation status of the gene promoter (p = 0.015). In the SCD group, the proportion of individuals whose ABCA1 gene promoter is methylated is statistically significantly higher compared to the control group (p = 0.020; OR = 5.86; 95% CI (1.28–26.89)).Conclusion. Methylation of the promoter of the ABCA1 gene is associated with sudden cardiac death. |
| first_indexed | 2024-04-10T01:04:56Z |
| format | Article |
| id | doaj.art-14fb2a2dee25450192d002c1ee67790c |
| institution | Directory Open Access Journal |
| issn | 1682-0363 1819-3684 |
| language | English |
| last_indexed | 2024-04-10T01:04:56Z |
| publishDate | 2021-01-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj.art-14fb2a2dee25450192d002c1ee67790c2023-03-13T09:58:26ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842021-01-01194808510.20538/1682-0363-2020-4-80-852725ABCA1 gene promoter methylation and sudden cardiac deathA. A. Ivanova0A. A. Gurazheva1E. I. Akinshina2S. V. Maksimova3S. K. Malyutina4V. P. Novoselov5I. A. Rodina6O. V. Khamovich7V. N. Maksimov8Научно-исследовательский институт терапии и профилактической медицины (НИИТПМ) – филиал Федерального исследовательского центра «Институт цитологии и генетики» Сибирского отделения Российской академии наук (ФИЦ ИЦиГ СО РАН)Научно-исследовательский институт терапии и профилактической медицины (НИИТПМ) – филиал Федерального исследовательского центра «Институт цитологии и генетики» Сибирского отделения Российской академии наук (ФИЦ ИЦиГ СО РАН)Научно-исследовательский институт терапии и профилактической медицины (НИИТПМ) – филиал Федерального исследовательского центра «Институт цитологии и генетики» Сибирского отделения Российской академии наук (ФИЦ ИЦиГ СО РАН)Новосибирский государственный медицинский университет (НГМУ)Научно-исследовательский институт терапии и профилактической медицины (НИИТПМ) – филиал Федерального исследовательского центра «Институт цитологии и генетики» Сибирского отделения Российской академии наук (ФИЦ ИЦиГ СО РАН)Новосибирское областное клиническое бюро судебно-медицинской экспертизы (НОКБСМЭ)Новосибирское областное клиническое бюро судебно-медицинской экспертизы (НОКБСМЭ)Новосибирское областное клиническое бюро судебно-медицинской экспертизы (НОКБСМЭ)Научно-исследовательский институт терапии и профилактической медицины (НИИТПМ) – филиал Федерального исследовательского центра «Институт цитологии и генетики» Сибирского отделения Российской академии наук (ФИЦ ИЦиГ СО РАН)Aim. A study of the association of the methylation of the promoter of the ABCA1 gene with sudden cardiac death(SCD).Materials and methods. The study design is based on the case-control principle. The SCD group included 150 men (mean age (46.7 ± 9.2) years) who died of sudden cardiac death according to forensic medical examination data (the main pathological diagnoses are acute circulatory failure, acute coronary insufficiency). The control group included 150 men (mean age (42.6 ± 1.2) years) who died suddenly, but not due to cardiovascular pathology. DNA was isolated by phenol-chloroform extraction from myocardial tissue. The methylation status of the ABCA1 gene promoter was assessed by methyl-specific polymerase chain reaction. The results obtained were statistically processed in SPSS 16.0 using Pearson’s test and Fisher’s test with Yates’ correction for continuity. P < 0.05 was used as a level of significance.Results. Comparing the groups revealed statistically significant differences in the methylation status of the gene promoter (p = 0.015). In the SCD group, the proportion of individuals whose ABCA1 gene promoter is methylated is statistically significantly higher compared to the control group (p = 0.020; OR = 5.86; 95% CI (1.28–26.89)).Conclusion. Methylation of the promoter of the ABCA1 gene is associated with sudden cardiac death.https://bulletin.ssmu.ru/jour/article/view/4153внезапная сердечная смертьметилированиеabca1промотор |
| spellingShingle | A. A. Ivanova A. A. Gurazheva E. I. Akinshina S. V. Maksimova S. K. Malyutina V. P. Novoselov I. A. Rodina O. V. Khamovich V. N. Maksimov ABCA1 gene promoter methylation and sudden cardiac death Бюллетень сибирской медицины внезапная сердечная смерть метилирование abca1 промотор |
| title | ABCA1 gene promoter methylation and sudden cardiac death |
| title_full | ABCA1 gene promoter methylation and sudden cardiac death |
| title_fullStr | ABCA1 gene promoter methylation and sudden cardiac death |
| title_full_unstemmed | ABCA1 gene promoter methylation and sudden cardiac death |
| title_short | ABCA1 gene promoter methylation and sudden cardiac death |
| title_sort | abca1 gene promoter methylation and sudden cardiac death |
| topic | внезапная сердечная смерть метилирование abca1 промотор |
| url | https://bulletin.ssmu.ru/jour/article/view/4153 |
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