The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions
Ferritin, a spherically shaped protein complex, is responsible for iron storage in bacteria, plants, animals, and humans. Various ferritin iron core compositions in organisms are associated with specific living requirements, health state, and different biochemical roles of ferritin isomers. Magnetof...
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MDPI AG
2021-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/22/6960 |
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| author | Lucia Balejčíková Karel Saksl Jozef Kováč Anne Martel Vasil M. Garamus Mikhail V. Avdeev Viktor I. Petrenko László Almásy Peter Kopčanský |
| author_facet | Lucia Balejčíková Karel Saksl Jozef Kováč Anne Martel Vasil M. Garamus Mikhail V. Avdeev Viktor I. Petrenko László Almásy Peter Kopčanský |
| author_sort | Lucia Balejčíková |
| collection | DOAJ |
| description | Ferritin, a spherically shaped protein complex, is responsible for iron storage in bacteria, plants, animals, and humans. Various ferritin iron core compositions in organisms are associated with specific living requirements, health state, and different biochemical roles of ferritin isomers. Magnetoferritin, a synthetic ferritin derivative, serves as an artificial model system of unusual iron phase structures found in humans. We present the results of a complex structural study of magnetoferritins prepared by controlled in vitro synthesis. Using various complementary methods, it was observed that manipulation of the synthesis technology can improve the physicochemical parameters of the system, which is useful in applications. Thus, a higher synthesis temperature leads to an increase in magnetization due to the formation of the magnetite phase. An increase in the iron loading factor has a more pronounced impact on the protein shell structure in comparison with the pH of the aqueous medium. On the other hand, a higher loading factor at physiological temperature enhances the formation of an amorphous phase instead of magnetite crystallization. It was confirmed that the iron-overloading effect alone (observed during pathological events) cannot contribute to the formation of magnetite. |
| first_indexed | 2024-03-10T05:13:56Z |
| format | Article |
| id | doaj.art-14fd86fc1fa74d149209f302da826dc5 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T05:13:56Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-14fd86fc1fa74d149209f302da826dc52023-11-23T00:36:29ZengMDPI AGMolecules1420-30492021-11-012622696010.3390/molecules26226960The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal ConditionsLucia Balejčíková0Karel Saksl1Jozef Kováč2Anne Martel3Vasil M. Garamus4Mikhail V. Avdeev5Viktor I. Petrenko6László Almásy7Peter Kopčanský8Institute of Hydrology SAS, Dúbravská Cesta 9, 84104 Bratislava, SlovakiaInstitute of Materials Research Slovak Academy of Sciences, Watsonova 47, 04001 Košice, SlovakiaInstitute of Experimental Physic SAS, Watsonova 47, 04001 Košice, SlovakiaInstitut Laue-Langevin, 71 Avenue des Martyrs, 38042 Grenoble, FranceHelmholtz-Zentrum Hereon, Max-Planck-Street 1, 21502 Geesthacht, GermanyJoint Institute for Nuclear Research, Joliot-Curie Str. 6, 141980 Dubna, RussiaBCMaterials, Basque Centre for Materials, Applications and Nanostructures, 48940 Leioa, SpainInstitute for Energy Security and Environmental Safety, Centre for Energy Research, Konkoly Thege Mi-klos ut 29-33, 1121 Budapest, HungaryInstitute of Experimental Physic SAS, Watsonova 47, 04001 Košice, SlovakiaFerritin, a spherically shaped protein complex, is responsible for iron storage in bacteria, plants, animals, and humans. Various ferritin iron core compositions in organisms are associated with specific living requirements, health state, and different biochemical roles of ferritin isomers. Magnetoferritin, a synthetic ferritin derivative, serves as an artificial model system of unusual iron phase structures found in humans. We present the results of a complex structural study of magnetoferritins prepared by controlled in vitro synthesis. Using various complementary methods, it was observed that manipulation of the synthesis technology can improve the physicochemical parameters of the system, which is useful in applications. Thus, a higher synthesis temperature leads to an increase in magnetization due to the formation of the magnetite phase. An increase in the iron loading factor has a more pronounced impact on the protein shell structure in comparison with the pH of the aqueous medium. On the other hand, a higher loading factor at physiological temperature enhances the formation of an amorphous phase instead of magnetite crystallization. It was confirmed that the iron-overloading effect alone (observed during pathological events) cannot contribute to the formation of magnetite.https://www.mdpi.com/1420-3049/26/22/6960magnetoferritinmagnetiteloading factorprotein stabilityaqueous medium |
| spellingShingle | Lucia Balejčíková Karel Saksl Jozef Kováč Anne Martel Vasil M. Garamus Mikhail V. Avdeev Viktor I. Petrenko László Almásy Peter Kopčanský The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions Molecules magnetoferritin magnetite loading factor protein stability aqueous medium |
| title | The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions |
| title_full | The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions |
| title_fullStr | The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions |
| title_full_unstemmed | The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions |
| title_short | The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions |
| title_sort | impact of redox hydrolysis and dehydration chemistry on the structural and magnetic properties of magnetoferritin prepared in variable thermal conditions |
| topic | magnetoferritin magnetite loading factor protein stability aqueous medium |
| url | https://www.mdpi.com/1420-3049/26/22/6960 |
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