Transfusion associated unknown peak on HPLC chromatogram: A diagnostic dilemma
A pre-transfusion blood sample with a well-spread and well-stained peripheral smear is indispensible for diagnosis of hemolytic anemia. However, apparent hemoglobinopathies have been reported in previous literature. They are known to cause delay in diagnosis and treatment. We report a case of newly...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | Journal of Applied Hematology |
| Subjects: | |
| Online Access: | http://www.jahjournal.org/article.asp?issn=1658-5127;year=2014;volume=5;issue=1;spage=19;epage=22;aulast=Chauhan |
| Summary: | A pre-transfusion blood sample with a well-spread and well-stained peripheral smear is indispensible for diagnosis of hemolytic anemia. However, apparent hemoglobinopathies have been reported in previous literature. They are known to cause delay in diagnosis and treatment. We report a case of newly diagnosed β-thalassemia major infant, in which an abnormal peak was seen at retention time 4.71 minutes on Hemoglobin-High Performance Liquid Chromatography (Hb-HPLC). This peak was associated with history of recent blood transfusion and was confirmed by family study and with repeated values of HPLC. |
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| ISSN: | 1658-5127 |