Transfusion associated unknown peak on HPLC chromatogram: A diagnostic dilemma
A pre-transfusion blood sample with a well-spread and well-stained peripheral smear is indispensible for diagnosis of hemolytic anemia. However, apparent hemoglobinopathies have been reported in previous literature. They are known to cause delay in diagnosis and treatment. We report a case of newly...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | Journal of Applied Hematology |
| Subjects: | |
| Online Access: | http://www.jahjournal.org/article.asp?issn=1658-5127;year=2014;volume=5;issue=1;spage=19;epage=22;aulast=Chauhan |
| _version_ | 1819111485671997440 |
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| author | Richa Chauhan Sunita Sharma Preeti Rai Jagdish Chandra |
| author_facet | Richa Chauhan Sunita Sharma Preeti Rai Jagdish Chandra |
| author_sort | Richa Chauhan |
| collection | DOAJ |
| description | A pre-transfusion blood sample with a well-spread and well-stained peripheral smear is indispensible for diagnosis of hemolytic anemia. However, apparent hemoglobinopathies have been reported in previous literature. They are known to cause delay in diagnosis and treatment. We report a case of newly diagnosed β-thalassemia major infant, in which an abnormal peak was seen at retention time 4.71 minutes on Hemoglobin-High Performance Liquid Chromatography (Hb-HPLC). This peak was associated with history of recent blood transfusion and was confirmed by family study and with repeated values of HPLC. |
| first_indexed | 2024-12-22T03:58:22Z |
| format | Article |
| id | doaj.art-150035d7322b4b838f1a7d251cfcd860 |
| institution | Directory Open Access Journal |
| issn | 1658-5127 |
| language | English |
| last_indexed | 2024-12-22T03:58:22Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Applied Hematology |
| spelling | doaj.art-150035d7322b4b838f1a7d251cfcd8602022-12-21T18:39:48ZengWolters Kluwer Medknow PublicationsJournal of Applied Hematology1658-51272014-01-0151192210.4103/1658-5127.131821Transfusion associated unknown peak on HPLC chromatogram: A diagnostic dilemmaRicha ChauhanSunita SharmaPreeti RaiJagdish ChandraA pre-transfusion blood sample with a well-spread and well-stained peripheral smear is indispensible for diagnosis of hemolytic anemia. However, apparent hemoglobinopathies have been reported in previous literature. They are known to cause delay in diagnosis and treatment. We report a case of newly diagnosed β-thalassemia major infant, in which an abnormal peak was seen at retention time 4.71 minutes on Hemoglobin-High Performance Liquid Chromatography (Hb-HPLC). This peak was associated with history of recent blood transfusion and was confirmed by family study and with repeated values of HPLC.http://www.jahjournal.org/article.asp?issn=1658-5127;year=2014;volume=5;issue=1;spage=19;epage=22;aulast=ChauhanHemoglobinopathyhigh performance liquid chromatographytransfusionβ-thalassemia major |
| spellingShingle | Richa Chauhan Sunita Sharma Preeti Rai Jagdish Chandra Transfusion associated unknown peak on HPLC chromatogram: A diagnostic dilemma Journal of Applied Hematology Hemoglobinopathy high performance liquid chromatography transfusion β-thalassemia major |
| title | Transfusion associated unknown peak on HPLC chromatogram: A diagnostic dilemma |
| title_full | Transfusion associated unknown peak on HPLC chromatogram: A diagnostic dilemma |
| title_fullStr | Transfusion associated unknown peak on HPLC chromatogram: A diagnostic dilemma |
| title_full_unstemmed | Transfusion associated unknown peak on HPLC chromatogram: A diagnostic dilemma |
| title_short | Transfusion associated unknown peak on HPLC chromatogram: A diagnostic dilemma |
| title_sort | transfusion associated unknown peak on hplc chromatogram a diagnostic dilemma |
| topic | Hemoglobinopathy high performance liquid chromatography transfusion β-thalassemia major |
| url | http://www.jahjournal.org/article.asp?issn=1658-5127;year=2014;volume=5;issue=1;spage=19;epage=22;aulast=Chauhan |
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