<i>N</i>,<i>N</i>′-Diphenyl-1,4-phenylenediamine Antioxidant’s Potential Role in Enhancing the Pancreatic Antioxidant, Immunomodulatory, and Anti-Apoptotic Therapeutic Capabilities of Adipose-Derived Stem Cells in Type I Diabetic Rats
Mesenchymal stem cells (MSCs) are considered to be a promising therapeutic protocol for diabetes mellitus (DM) management. The latter is attributed to their differentiation potentiality to pancreatic β-cells, angiogenesis, and immune-modulatory capabilities by releasing various paracrine factors. In...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/1/58 |
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| author | Saad Shaaban Hemdan El-Shamy Mohamed Gouda Marwa K. Darwish Hany M. Abd El-Lateef Mai M. Khalaf Ehab I. El-Hallous Kholoud H. Radwan Hanan M. Rashwan Shady G. El-Sawah |
| author_facet | Saad Shaaban Hemdan El-Shamy Mohamed Gouda Marwa K. Darwish Hany M. Abd El-Lateef Mai M. Khalaf Ehab I. El-Hallous Kholoud H. Radwan Hanan M. Rashwan Shady G. El-Sawah |
| author_sort | Saad Shaaban |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) are considered to be a promising therapeutic protocol for diabetes mellitus (DM) management. The latter is attributed to their differentiation potentiality to pancreatic β-cells, angiogenesis, and immune-modulatory capabilities by releasing various paracrine factors. Interestingly, antioxidant co-administration increased the MSCs’ hypoglycemic and regenerative activities. Thus, this study aims to evaluate the therapeutic implication of type 1 DM after the co-administration of adipose tissue-derived-MSCs (AD-MSCs) and <i>N</i>,<i>N</i>′-d iphenyl-1,4-phenylenediamine (DPPD), compared to the single injection of either of them alone. In our four week long experiment, six rat groups were used as control, DPPD (250 mg/kg, i.p.), STZ-diabetic (D), D+DPPD, D+AD-MSCs (1 × 10<sup>6</sup> cell/rat, i.p.), and D+AD-MSCs+DPPD groups. Within this context, a single injection of AD-MSCs or DPPD into diabetic rats showed significant pancreatic anti-inflammatory, immunomodulation, antioxidant, and anti-apoptotic capacities, superior to AD-MSCs injection. However, AD-MSCs and DPPD co-administration into diabetic rats manifested the highest hypoglycemic and pancreatic regenerative activities in managing diabetes compared to the single shot of AD-MSCs or DPPD. These results highlight the synergetic role of DPPD as an antioxidant in enhancing AD-MSCs’ therapeutic applications. |
| first_indexed | 2024-03-09T13:46:49Z |
| format | Article |
| id | doaj.art-1500670bdf9d4607bb91c97a9ed73e3e |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T13:46:49Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-1500670bdf9d4607bb91c97a9ed73e3e2023-11-30T20:57:45ZengMDPI AGAntioxidants2076-39212022-12-011215810.3390/antiox12010058<i>N</i>,<i>N</i>′-Diphenyl-1,4-phenylenediamine Antioxidant’s Potential Role in Enhancing the Pancreatic Antioxidant, Immunomodulatory, and Anti-Apoptotic Therapeutic Capabilities of Adipose-Derived Stem Cells in Type I Diabetic RatsSaad Shaaban0Hemdan El-Shamy1Mohamed Gouda2Marwa K. Darwish3Hany M. Abd El-Lateef4Mai M. Khalaf5Ehab I. El-Hallous6Kholoud H. Radwan7Hanan M. Rashwan8Shady G. El-Sawah9Department of Chemistry, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa 31982, Saudi ArabiaDeanship of Student Affairs, King Faisal University, P.O. Box 380, Al-Ahsa 31982, Saudi ArabiaDepartment of Chemistry, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa 31982, Saudi ArabiaChemistry Department (Biochemistry Branch), Faculty of Science, Suez University, P.O. Box 43518, Suez 8151650, EgyptDepartment of Chemistry, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa 31982, Saudi ArabiaDepartment of Chemistry, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa 31982, Saudi ArabiaZoology Department, Faculty of Science, Arish University, El Arish 31111, EgyptDepartment of Biochemistry, Horus University in Egypt HUE, Damietta 7991164, EgyptZoology Department, Faculty of Science, Arish University, El Arish 31111, EgyptZoology Department, Faculty of Science, Arish University, El Arish 31111, EgyptMesenchymal stem cells (MSCs) are considered to be a promising therapeutic protocol for diabetes mellitus (DM) management. The latter is attributed to their differentiation potentiality to pancreatic β-cells, angiogenesis, and immune-modulatory capabilities by releasing various paracrine factors. Interestingly, antioxidant co-administration increased the MSCs’ hypoglycemic and regenerative activities. Thus, this study aims to evaluate the therapeutic implication of type 1 DM after the co-administration of adipose tissue-derived-MSCs (AD-MSCs) and <i>N</i>,<i>N</i>′-d iphenyl-1,4-phenylenediamine (DPPD), compared to the single injection of either of them alone. In our four week long experiment, six rat groups were used as control, DPPD (250 mg/kg, i.p.), STZ-diabetic (D), D+DPPD, D+AD-MSCs (1 × 10<sup>6</sup> cell/rat, i.p.), and D+AD-MSCs+DPPD groups. Within this context, a single injection of AD-MSCs or DPPD into diabetic rats showed significant pancreatic anti-inflammatory, immunomodulation, antioxidant, and anti-apoptotic capacities, superior to AD-MSCs injection. However, AD-MSCs and DPPD co-administration into diabetic rats manifested the highest hypoglycemic and pancreatic regenerative activities in managing diabetes compared to the single shot of AD-MSCs or DPPD. These results highlight the synergetic role of DPPD as an antioxidant in enhancing AD-MSCs’ therapeutic applications.https://www.mdpi.com/2076-3921/12/1/58apoptosisantioxidantsinflammationdiabetesstem cellsoxidative stress |
| spellingShingle | Saad Shaaban Hemdan El-Shamy Mohamed Gouda Marwa K. Darwish Hany M. Abd El-Lateef Mai M. Khalaf Ehab I. El-Hallous Kholoud H. Radwan Hanan M. Rashwan Shady G. El-Sawah <i>N</i>,<i>N</i>′-Diphenyl-1,4-phenylenediamine Antioxidant’s Potential Role in Enhancing the Pancreatic Antioxidant, Immunomodulatory, and Anti-Apoptotic Therapeutic Capabilities of Adipose-Derived Stem Cells in Type I Diabetic Rats Antioxidants apoptosis antioxidants inflammation diabetes stem cells oxidative stress |
| title | <i>N</i>,<i>N</i>′-Diphenyl-1,4-phenylenediamine Antioxidant’s Potential Role in Enhancing the Pancreatic Antioxidant, Immunomodulatory, and Anti-Apoptotic Therapeutic Capabilities of Adipose-Derived Stem Cells in Type I Diabetic Rats |
| title_full | <i>N</i>,<i>N</i>′-Diphenyl-1,4-phenylenediamine Antioxidant’s Potential Role in Enhancing the Pancreatic Antioxidant, Immunomodulatory, and Anti-Apoptotic Therapeutic Capabilities of Adipose-Derived Stem Cells in Type I Diabetic Rats |
| title_fullStr | <i>N</i>,<i>N</i>′-Diphenyl-1,4-phenylenediamine Antioxidant’s Potential Role in Enhancing the Pancreatic Antioxidant, Immunomodulatory, and Anti-Apoptotic Therapeutic Capabilities of Adipose-Derived Stem Cells in Type I Diabetic Rats |
| title_full_unstemmed | <i>N</i>,<i>N</i>′-Diphenyl-1,4-phenylenediamine Antioxidant’s Potential Role in Enhancing the Pancreatic Antioxidant, Immunomodulatory, and Anti-Apoptotic Therapeutic Capabilities of Adipose-Derived Stem Cells in Type I Diabetic Rats |
| title_short | <i>N</i>,<i>N</i>′-Diphenyl-1,4-phenylenediamine Antioxidant’s Potential Role in Enhancing the Pancreatic Antioxidant, Immunomodulatory, and Anti-Apoptotic Therapeutic Capabilities of Adipose-Derived Stem Cells in Type I Diabetic Rats |
| title_sort | i n i i n i diphenyl 1 4 phenylenediamine antioxidant s potential role in enhancing the pancreatic antioxidant immunomodulatory and anti apoptotic therapeutic capabilities of adipose derived stem cells in type i diabetic rats |
| topic | apoptosis antioxidants inflammation diabetes stem cells oxidative stress |
| url | https://www.mdpi.com/2076-3921/12/1/58 |
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