Comparative studies on two tests for detection of avian influenza a virus and Newcastle disease virus in co-infected guinea fowl (Numida meleagris)
Thirty four oropharyngeal swabs were collected from guinea fowl infected with a low-pathogenicity avian influenza A virus Н6N2 (LPAIV H6N2) and vaccinated with a lentogenic NDV strain La Sota. All samples were examined in HI test after attempts for isolation of viruses in 9-day-old chick embryos (CЕ...
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| Format: | Article |
| Language: | English |
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Trakia University
2018-03-01
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| Series: | Trakia Journal of Sciences |
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| Online Access: | http://tru.uni-sz.bg/tsj/N%201,%20Vol.16,%202018/Iv.Zarkov.pdf |
| _version_ | 1811343336021164032 |
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| author | Iv. Zarkov |
| author_facet | Iv. Zarkov |
| author_sort | Iv. Zarkov |
| collection | DOAJ |
| description | Thirty four oropharyngeal swabs were collected from guinea fowl infected with a low-pathogenicity avian influenza A virus Н6N2 (LPAIV H6N2) and vaccinated with a lentogenic NDV strain La Sota. All samples were examined in HI test after attempts for isolation of viruses in 9-day-old chick embryos (CЕ) and by means of AIV-NDV rapid Ag kit (RapiGEN, South Korea). The results demonstrated that the rapid test could be used for guinea fowl despite its lower sensitivity of 91.67 % as compared to the HI test after isolation in CE. The test specificity was 100 % indicating that it could distinguish both viruses in co-infections. |
| first_indexed | 2024-04-13T19:28:40Z |
| format | Article |
| id | doaj.art-150422dfad18422b940c5bcba344f271 |
| institution | Directory Open Access Journal |
| issn | 1312-1723 1313-3551 |
| language | English |
| last_indexed | 2024-04-13T19:28:40Z |
| publishDate | 2018-03-01 |
| publisher | Trakia University |
| record_format | Article |
| series | Trakia Journal of Sciences |
| spelling | doaj.art-150422dfad18422b940c5bcba344f2712022-12-22T02:33:16ZengTrakia UniversityTrakia Journal of Sciences1312-17231313-35512018-03-0116171010.15547/tjs.2018.01.002Comparative studies on two tests for detection of avian influenza a virus and Newcastle disease virus in co-infected guinea fowl (Numida meleagris)Iv. ZarkovThirty four oropharyngeal swabs were collected from guinea fowl infected with a low-pathogenicity avian influenza A virus Н6N2 (LPAIV H6N2) and vaccinated with a lentogenic NDV strain La Sota. All samples were examined in HI test after attempts for isolation of viruses in 9-day-old chick embryos (CЕ) and by means of AIV-NDV rapid Ag kit (RapiGEN, South Korea). The results demonstrated that the rapid test could be used for guinea fowl despite its lower sensitivity of 91.67 % as compared to the HI test after isolation in CE. The test specificity was 100 % indicating that it could distinguish both viruses in co-infections.http://tru.uni-sz.bg/tsj/N%201,%20Vol.16,%202018/Iv.Zarkov.pdfAvian influenza virusNewcastle disease virus (NDV)AIV-NDV Rapid Ag kitHI testvirus isolation |
| spellingShingle | Iv. Zarkov Comparative studies on two tests for detection of avian influenza a virus and Newcastle disease virus in co-infected guinea fowl (Numida meleagris) Trakia Journal of Sciences Avian influenza virus Newcastle disease virus (NDV) AIV-NDV Rapid Ag kit HI test virus isolation |
| title | Comparative studies on two tests for detection of avian influenza a virus and Newcastle disease virus in co-infected guinea fowl (Numida meleagris) |
| title_full | Comparative studies on two tests for detection of avian influenza a virus and Newcastle disease virus in co-infected guinea fowl (Numida meleagris) |
| title_fullStr | Comparative studies on two tests for detection of avian influenza a virus and Newcastle disease virus in co-infected guinea fowl (Numida meleagris) |
| title_full_unstemmed | Comparative studies on two tests for detection of avian influenza a virus and Newcastle disease virus in co-infected guinea fowl (Numida meleagris) |
| title_short | Comparative studies on two tests for detection of avian influenza a virus and Newcastle disease virus in co-infected guinea fowl (Numida meleagris) |
| title_sort | comparative studies on two tests for detection of avian influenza a virus and newcastle disease virus in co infected guinea fowl numida meleagris |
| topic | Avian influenza virus Newcastle disease virus (NDV) AIV-NDV Rapid Ag kit HI test virus isolation |
| url | http://tru.uni-sz.bg/tsj/N%201,%20Vol.16,%202018/Iv.Zarkov.pdf |
| work_keys_str_mv | AT ivzarkov comparativestudiesontwotestsfordetectionofavianinfluenzaavirusandnewcastlediseasevirusincoinfectedguineafowlnumidameleagris |