Anti-Inflammatory Activity of the Wild Mushroom, <i>Echinodontium tinctorium,</i> in RAW264.7 Macrophage Cells and Mouse Microcirculation
The aim of this study was to investigate the anti-inflammatory activity of a previously un-studied wild mushroom, <i>Echinodontium tinctorium</i>, collected from the forests of north-central British Columbia. The lipopolysaccharide (LPS)-induced RAW264.7 macrophage model was used to stud...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/19/3509 |
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| author | Sumreen Javed Wai Ming Li Mehreen Zeb Almas Yaqoob Linda E. Tackaberry Hugues B. Massicotte Keith N. Egger Peter C.K. Cheung Geoffrey W. Payne Chow H. Lee |
| author_facet | Sumreen Javed Wai Ming Li Mehreen Zeb Almas Yaqoob Linda E. Tackaberry Hugues B. Massicotte Keith N. Egger Peter C.K. Cheung Geoffrey W. Payne Chow H. Lee |
| author_sort | Sumreen Javed |
| collection | DOAJ |
| description | The aim of this study was to investigate the anti-inflammatory activity of a previously un-studied wild mushroom, <i>Echinodontium tinctorium</i>, collected from the forests of north-central British Columbia. The lipopolysaccharide (LPS)-induced RAW264.7 macrophage model was used to study the in vitro anti-inflammatory activity. The crude alkaline extract demonstrated potent anti-inflammatory activity, and was further purified using a “bio-activity-guided-purification” approach. The size-exclusion and ion-exchange chromatography yielded a water-soluble anti-inflammatory polysaccharide (AIPetinc). AIPetinc has an average molecular weight of 5 kDa, and is a heteroglucan composed of mainly glucose (88.6%) with a small amount of galactose (4.0%), mannose (4.4%), fucose (0.7%), and xylose (2.3%). In in vivo settings, AIPetinc restored the histamine-induced inflammatory event in mouse gluteus maximus muscle, thus confirming its anti-inflammatory activity in an animal model. This study constitutes the first report on the bioactivity of <i>Echinodontium tinctorium</i>, and highlights the potential medicinal benefits of fungi from the wild forests of northern British Columbia. Furthermore, it also reiterates the need to explore natural resources for alternative treatment to modern world diseases. |
| first_indexed | 2024-12-10T09:30:53Z |
| format | Article |
| id | doaj.art-1504f8928d06413ba932a555c2b12f8e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T09:30:53Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1504f8928d06413ba932a555c2b12f8e2022-12-22T01:54:21ZengMDPI AGMolecules1420-30492019-09-012419350910.3390/molecules24193509molecules24193509Anti-Inflammatory Activity of the Wild Mushroom, <i>Echinodontium tinctorium,</i> in RAW264.7 Macrophage Cells and Mouse MicrocirculationSumreen Javed0Wai Ming Li1Mehreen Zeb2Almas Yaqoob3Linda E. Tackaberry4Hugues B. Massicotte5Keith N. Egger6Peter C.K. Cheung7Geoffrey W. Payne8Chow H. Lee9Chemistry and Biochemistry Program, University of Northern British Columbia, Prince George, BC V2N 4Z9, CanadaChemistry and Biochemistry Program, University of Northern British Columbia, Prince George, BC V2N 4Z9, CanadaChemistry and Biochemistry Program, University of Northern British Columbia, Prince George, BC V2N 4Z9, CanadaChemistry and Biochemistry Program, University of Northern British Columbia, Prince George, BC V2N 4Z9, CanadaEcosystem Science and Management Program, University of Northern British Columbia, Prince George, BC V2N 4Z9, CanadaEcosystem Science and Management Program, University of Northern British Columbia, Prince George, BC V2N 4Z9, CanadaEcosystem Science and Management Program, University of Northern British Columbia, Prince George, BC V2N 4Z9, CanadaFood and Nutritional Sciences Program, School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, ChinaNorthern Medical Program, University of Northern British Columbia, Prince George, BC V2N 4Z9, CanadaChemistry and Biochemistry Program, University of Northern British Columbia, Prince George, BC V2N 4Z9, CanadaThe aim of this study was to investigate the anti-inflammatory activity of a previously un-studied wild mushroom, <i>Echinodontium tinctorium</i>, collected from the forests of north-central British Columbia. The lipopolysaccharide (LPS)-induced RAW264.7 macrophage model was used to study the in vitro anti-inflammatory activity. The crude alkaline extract demonstrated potent anti-inflammatory activity, and was further purified using a “bio-activity-guided-purification” approach. The size-exclusion and ion-exchange chromatography yielded a water-soluble anti-inflammatory polysaccharide (AIPetinc). AIPetinc has an average molecular weight of 5 kDa, and is a heteroglucan composed of mainly glucose (88.6%) with a small amount of galactose (4.0%), mannose (4.4%), fucose (0.7%), and xylose (2.3%). In in vivo settings, AIPetinc restored the histamine-induced inflammatory event in mouse gluteus maximus muscle, thus confirming its anti-inflammatory activity in an animal model. This study constitutes the first report on the bioactivity of <i>Echinodontium tinctorium</i>, and highlights the potential medicinal benefits of fungi from the wild forests of northern British Columbia. Furthermore, it also reiterates the need to explore natural resources for alternative treatment to modern world diseases.https://www.mdpi.com/1420-3049/24/19/3509<i>echinodontium tinctorium</i>polysaccharideanti-inflammationmushroombritish columbia |
| spellingShingle | Sumreen Javed Wai Ming Li Mehreen Zeb Almas Yaqoob Linda E. Tackaberry Hugues B. Massicotte Keith N. Egger Peter C.K. Cheung Geoffrey W. Payne Chow H. Lee Anti-Inflammatory Activity of the Wild Mushroom, <i>Echinodontium tinctorium,</i> in RAW264.7 Macrophage Cells and Mouse Microcirculation Molecules <i>echinodontium tinctorium</i> polysaccharide anti-inflammation mushroom british columbia |
| title | Anti-Inflammatory Activity of the Wild Mushroom, <i>Echinodontium tinctorium,</i> in RAW264.7 Macrophage Cells and Mouse Microcirculation |
| title_full | Anti-Inflammatory Activity of the Wild Mushroom, <i>Echinodontium tinctorium,</i> in RAW264.7 Macrophage Cells and Mouse Microcirculation |
| title_fullStr | Anti-Inflammatory Activity of the Wild Mushroom, <i>Echinodontium tinctorium,</i> in RAW264.7 Macrophage Cells and Mouse Microcirculation |
| title_full_unstemmed | Anti-Inflammatory Activity of the Wild Mushroom, <i>Echinodontium tinctorium,</i> in RAW264.7 Macrophage Cells and Mouse Microcirculation |
| title_short | Anti-Inflammatory Activity of the Wild Mushroom, <i>Echinodontium tinctorium,</i> in RAW264.7 Macrophage Cells and Mouse Microcirculation |
| title_sort | anti inflammatory activity of the wild mushroom i echinodontium tinctorium i in raw264 7 macrophage cells and mouse microcirculation |
| topic | <i>echinodontium tinctorium</i> polysaccharide anti-inflammation mushroom british columbia |
| url | https://www.mdpi.com/1420-3049/24/19/3509 |
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