Potential Antimicrobial and Anticancer Activities of an Ethanol Extract from <i>Bouea macrophylla</i>
<i>Bouea macrophylla</i> is a tree widely grown throughout South East Asia. It is used in folk medicine for the treatment of various illnesses. The present study aimed to identify the chemical constituents and to test the antimicrobial and anticancer activities of an ethanol extract from...
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MDPI AG
2020-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/8/1996 |
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| author | Ngoc Hong Nguyen Thuy Trang Nguyen Phu Cuong Ma Qui Thanh Hoai Ta Thuc-Huy Duong Van Giau Vo |
| author_facet | Ngoc Hong Nguyen Thuy Trang Nguyen Phu Cuong Ma Qui Thanh Hoai Ta Thuc-Huy Duong Van Giau Vo |
| author_sort | Ngoc Hong Nguyen |
| collection | DOAJ |
| description | <i>Bouea macrophylla</i> is a tree widely grown throughout South East Asia. It is used in folk medicine for the treatment of various illnesses. The present study aimed to identify the chemical constituents and to test the antimicrobial and anticancer activities of an ethanol extract from <i>B. macrophylla</i> leaves. The extract exhibited excellent antibacterial properties against 9 out of 10 target microorganisms. including four Gram-negative bacteria (<i>Escherichia coli, Shigella flexneri, Vibrio cholera,</i> and <i>Pseudomonas aeruginosa)</i> and four Gram-positive bacteria (<i>Staphylococcus aureus, Listeria monocytogenes, Enterococcus faecalis,</i> and <i>Bacillus cereus</i>), as well as a fungus (<i>Candida albicans).</i> In addition, the extract was also tested on HeLa and human colorectal carcinoma (HCT116) cells to evaluate its cytostatic effects. The ethanol extract was able to inhibit the proliferation of HeLa and HCT116 cells, showing IC<sub>50</sub> = 24 ± 0.8 and 28 ± 0.9 µg/mL, respectively, whereas the IC<sub>50</sub> values of doxorubicin (standard) were 13.6 ± 1.3 and 15.8 ± 1.1 µg/mL respectively. Also, we identified various bioactive compounds in the extract such as polyphenols, flavonoids, caryophyllene, phytol, and trans-geranylgeraniol by GC-MS, which could contribute to the extract’s biological activities. Therefore, our findings strongly indicate that the constituents of the <i>B. macrophylla</i> ethanol extract could be active against the tested bacteria and fungi as well as cancer cells. Further investigation is needed to understand the mechanisms mediating the antimicrobial and anticancer effects and identify signaling pathways that could be targeted for therapeutic application. |
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| format | Article |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T20:15:05Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1507b240c4c0433fa6dfabcc726631732023-11-19T22:35:52ZengMDPI AGMolecules1420-30492020-04-01258199610.3390/molecules25081996Potential Antimicrobial and Anticancer Activities of an Ethanol Extract from <i>Bouea macrophylla</i>Ngoc Hong Nguyen0Thuy Trang Nguyen1Phu Cuong Ma2Qui Thanh Hoai Ta3Thuc-Huy Duong4Van Giau Vo5CirTech Institute, Ho Chi Minh City University of Technology, Ho Chi Minh City 700000, VietnamFaculty of Pharmacy, Ho Chi Minh City University of Technology, Ho Chi Minh City 700000, VietnamInstitute of Applied Science, HCMC University of Technology (HUTECH), Ho Chi Minh City 700000, VietnamInstitute of Research and Development, Duy Tan University, Danang 550000, VietnamDepartment of Organic Chemistry, University of Education, Ho Chi Minh City 700000, VietnamBionanotechnology Research Group, Ton Duc Thang University, Ho Chi Minh City 700000, Vietnam<i>Bouea macrophylla</i> is a tree widely grown throughout South East Asia. It is used in folk medicine for the treatment of various illnesses. The present study aimed to identify the chemical constituents and to test the antimicrobial and anticancer activities of an ethanol extract from <i>B. macrophylla</i> leaves. The extract exhibited excellent antibacterial properties against 9 out of 10 target microorganisms. including four Gram-negative bacteria (<i>Escherichia coli, Shigella flexneri, Vibrio cholera,</i> and <i>Pseudomonas aeruginosa)</i> and four Gram-positive bacteria (<i>Staphylococcus aureus, Listeria monocytogenes, Enterococcus faecalis,</i> and <i>Bacillus cereus</i>), as well as a fungus (<i>Candida albicans).</i> In addition, the extract was also tested on HeLa and human colorectal carcinoma (HCT116) cells to evaluate its cytostatic effects. The ethanol extract was able to inhibit the proliferation of HeLa and HCT116 cells, showing IC<sub>50</sub> = 24 ± 0.8 and 28 ± 0.9 µg/mL, respectively, whereas the IC<sub>50</sub> values of doxorubicin (standard) were 13.6 ± 1.3 and 15.8 ± 1.1 µg/mL respectively. Also, we identified various bioactive compounds in the extract such as polyphenols, flavonoids, caryophyllene, phytol, and trans-geranylgeraniol by GC-MS, which could contribute to the extract’s biological activities. Therefore, our findings strongly indicate that the constituents of the <i>B. macrophylla</i> ethanol extract could be active against the tested bacteria and fungi as well as cancer cells. Further investigation is needed to understand the mechanisms mediating the antimicrobial and anticancer effects and identify signaling pathways that could be targeted for therapeutic application.https://www.mdpi.com/1420-3049/25/8/1996<i>Bouea macrophylla</i>extractantimicrobial activityHeLaHCT116 |
| spellingShingle | Ngoc Hong Nguyen Thuy Trang Nguyen Phu Cuong Ma Qui Thanh Hoai Ta Thuc-Huy Duong Van Giau Vo Potential Antimicrobial and Anticancer Activities of an Ethanol Extract from <i>Bouea macrophylla</i> Molecules <i>Bouea macrophylla</i> extract antimicrobial activity HeLa HCT116 |
| title | Potential Antimicrobial and Anticancer Activities of an Ethanol Extract from <i>Bouea macrophylla</i> |
| title_full | Potential Antimicrobial and Anticancer Activities of an Ethanol Extract from <i>Bouea macrophylla</i> |
| title_fullStr | Potential Antimicrobial and Anticancer Activities of an Ethanol Extract from <i>Bouea macrophylla</i> |
| title_full_unstemmed | Potential Antimicrobial and Anticancer Activities of an Ethanol Extract from <i>Bouea macrophylla</i> |
| title_short | Potential Antimicrobial and Anticancer Activities of an Ethanol Extract from <i>Bouea macrophylla</i> |
| title_sort | potential antimicrobial and anticancer activities of an ethanol extract from i bouea macrophylla i |
| topic | <i>Bouea macrophylla</i> extract antimicrobial activity HeLa HCT116 |
| url | https://www.mdpi.com/1420-3049/25/8/1996 |
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