N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease
Abstract Background Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment. However, considering its heterogeneity, subjects with similar spirometric parameters do not necessarily have the same functional status. To overcome this limitati...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-11-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12967-018-1695-0 |
| _version_ | 1811285343944572928 |
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| author | Tamara Pavić Dario Dilber Domagoj Kifer Najda Selak Toma Keser Đivo Ljubičić Andrea Vukić Dugac Gordan Lauc Lada Rumora Olga Gornik |
| author_facet | Tamara Pavić Dario Dilber Domagoj Kifer Najda Selak Toma Keser Đivo Ljubičić Andrea Vukić Dugac Gordan Lauc Lada Rumora Olga Gornik |
| author_sort | Tamara Pavić |
| collection | DOAJ |
| description | Abstract Background Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment. However, considering its heterogeneity, subjects with similar spirometric parameters do not necessarily have the same functional status. To overcome this limitation novel biomarkers for COPD have been investigated. Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls. Methods Both the total plasma protein and IgG N-glycome have been profiled in the total of 137 patients with COPD and 95 matching controls from Croatia. Replication cohort consisted of 61 subjects with COPD and 148 controls recruited at another Croatian medical centre. Results Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms). We also observed a significant decline in plasma monogalactosylated species, and the same change replicated in IgG glycome. N-glycans also showed value in distinguishing subjects in different COPD GOLD stages, where the relative abundance of more complex glycan structures increased as the disease progressed. Glycans also showed statistically significant associations with the frequency of exacerbations and demonstrated to be affected by smoking, which is the major risk factor for COPD development. Conclusions This study showed that complexity of glycans associates with COPD, mirroring also the disease severity. Moreover, changes in N-glycome associate with exacerbation frequency and are affected by smoking. In general, this study provided new insights into plasma protein and IgG N-glycome changes occurring in COPD and pointed out potential novel markers of the disease progression and severity. |
| first_indexed | 2024-04-13T02:42:48Z |
| format | Article |
| id | doaj.art-15099fa04584425fb97f780738a00596 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-04-13T02:42:48Z |
| publishDate | 2018-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-15099fa04584425fb97f780738a005962022-12-22T03:06:08ZengBMCJournal of Translational Medicine1479-58762018-11-0116111510.1186/s12967-018-1695-0N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary diseaseTamara Pavić0Dario Dilber1Domagoj Kifer2Najda Selak3Toma Keser4Đivo Ljubičić5Andrea Vukić Dugac6Gordan Lauc7Lada Rumora8Olga Gornik9Faculty of Pharmacy and Biochemistry, University of ZagrebDeparment of Cardiology, County Hospital ČakovecFaculty of Pharmacy and Biochemistry, University of ZagrebFaculty of Pharmacy and Biochemistry, University of ZagrebFaculty of Pharmacy and Biochemistry, University of ZagrebDepartment of Pulmonology, Clinical Hospital DubravaClinical Department for Lung Diseases Jordanovac, University Hospital CentreFaculty of Pharmacy and Biochemistry, University of ZagrebFaculty of Pharmacy and Biochemistry, University of ZagrebFaculty of Pharmacy and Biochemistry, University of ZagrebAbstract Background Chronic obstructive pulmonary disease (COPD) is a complex condition, whose diagnosis requires spirometric assessment. However, considering its heterogeneity, subjects with similar spirometric parameters do not necessarily have the same functional status. To overcome this limitation novel biomarkers for COPD have been investigated. Therefore, we aimed to explore the potential value of N-glycans as COPD biomarkers and to examine the individual variation of plasma protein and immunoglobulin G (IgG) glycosylation profiles in subjects with COPD and healthy controls. Methods Both the total plasma protein and IgG N-glycome have been profiled in the total of 137 patients with COPD and 95 matching controls from Croatia. Replication cohort consisted of 61 subjects with COPD and 148 controls recruited at another Croatian medical centre. Results Plasma protein N-glycome in COPD subjects exhibited significant decrease in low branched and conversely, an increase in more complex glycan structures (tetragalactosylated, trisialylated, tetrasialylated and antennary fucosylated glycoforms). We also observed a significant decline in plasma monogalactosylated species, and the same change replicated in IgG glycome. N-glycans also showed value in distinguishing subjects in different COPD GOLD stages, where the relative abundance of more complex glycan structures increased as the disease progressed. Glycans also showed statistically significant associations with the frequency of exacerbations and demonstrated to be affected by smoking, which is the major risk factor for COPD development. Conclusions This study showed that complexity of glycans associates with COPD, mirroring also the disease severity. Moreover, changes in N-glycome associate with exacerbation frequency and are affected by smoking. In general, this study provided new insights into plasma protein and IgG N-glycome changes occurring in COPD and pointed out potential novel markers of the disease progression and severity.http://link.springer.com/article/10.1186/s12967-018-1695-0N-glycosylationCOPDBiomarkersPlasma glycoproteinsImmunoglobulin G |
| spellingShingle | Tamara Pavić Dario Dilber Domagoj Kifer Najda Selak Toma Keser Đivo Ljubičić Andrea Vukić Dugac Gordan Lauc Lada Rumora Olga Gornik N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease Journal of Translational Medicine N-glycosylation COPD Biomarkers Plasma glycoproteins Immunoglobulin G |
| title | N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease |
| title_full | N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease |
| title_fullStr | N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease |
| title_full_unstemmed | N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease |
| title_short | N-glycosylation patterns of plasma proteins and immunoglobulin G in chronic obstructive pulmonary disease |
| title_sort | n glycosylation patterns of plasma proteins and immunoglobulin g in chronic obstructive pulmonary disease |
| topic | N-glycosylation COPD Biomarkers Plasma glycoproteins Immunoglobulin G |
| url | http://link.springer.com/article/10.1186/s12967-018-1695-0 |
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