Whole-genome sequencing of Listeria monocytogenes isolated from the first listeriosis foodborne outbreak in South Korea
Listeria monocytogenes is a foodborne pathogen that causes listeriosis in humans with severe symptoms. In South Korea, listeriosis had only been reported sporadically among hospitalized patients until the first foodborne outbreak occurred in 2018. In this study, a L. monocytogenes strain responsible...
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| Format: | Article |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1182090/full |
| _version_ | 1827935507247529984 |
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| author | Seung Hun Lee Sangmi Lee Sang Hun Park Ok Kyung Koo |
| author_facet | Seung Hun Lee Sangmi Lee Sang Hun Park Ok Kyung Koo |
| author_sort | Seung Hun Lee |
| collection | DOAJ |
| description | Listeria monocytogenes is a foodborne pathogen that causes listeriosis in humans with severe symptoms. In South Korea, listeriosis had only been reported sporadically among hospitalized patients until the first foodborne outbreak occurred in 2018. In this study, a L. monocytogenes strain responsible for this outbreak (FSCNU0110) was characterized via whole genome sequencing and compared with publicly available L. monocytogenes genomes of the same clonal complex (CC). Strain FSCNU0110 belonged to multilocus sequence typing (MLST)-based sequence type 224 and CC224, and core genome MLST-based sublineage 6,178. The strain harbored tetracycline resistance gene tetM, four other antibiotic resistance genes, and 64 virulence genes, including Listeria pathogenicity island 1 (LIPI-1) and LIPI-3. Interestingly, llsX in LIPI-3 exhibited a characteristic SNP (deletion of A in position 4, resulting in a premature stop codon) that was missing among all CC224 strains isolated overseas but was conserved among those from South Korea. In addition, the tetM gene was also detected only in a subset of CC224 strains from South Korea. These findings will provide an essential basis for assessing the characteristics of CC224 strains in South Korea that have shown a potential to cause listeriosis outbreaks. |
| first_indexed | 2024-03-13T07:55:47Z |
| format | Article |
| id | doaj.art-150b9a7ac3ce420483bfabcdd22ee4e6 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-13T07:55:47Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-150b9a7ac3ce420483bfabcdd22ee4e62023-06-02T05:17:42ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-06-011410.3389/fmicb.2023.11820901182090Whole-genome sequencing of Listeria monocytogenes isolated from the first listeriosis foodborne outbreak in South KoreaSeung Hun Lee0Sangmi Lee1Sang Hun Park2Ok Kyung Koo3Department of Food and Nutrition, Gyeongsang National University, Jinju, Republic of KoreaDepartment of Food and Nutrition, Chungbuk National University, Cheongju, Republic of KoreaSeoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of KoreaDepartment of Food Science and Technology, Chungnam National University, Daejeon, Republic of KoreaListeria monocytogenes is a foodborne pathogen that causes listeriosis in humans with severe symptoms. In South Korea, listeriosis had only been reported sporadically among hospitalized patients until the first foodborne outbreak occurred in 2018. In this study, a L. monocytogenes strain responsible for this outbreak (FSCNU0110) was characterized via whole genome sequencing and compared with publicly available L. monocytogenes genomes of the same clonal complex (CC). Strain FSCNU0110 belonged to multilocus sequence typing (MLST)-based sequence type 224 and CC224, and core genome MLST-based sublineage 6,178. The strain harbored tetracycline resistance gene tetM, four other antibiotic resistance genes, and 64 virulence genes, including Listeria pathogenicity island 1 (LIPI-1) and LIPI-3. Interestingly, llsX in LIPI-3 exhibited a characteristic SNP (deletion of A in position 4, resulting in a premature stop codon) that was missing among all CC224 strains isolated overseas but was conserved among those from South Korea. In addition, the tetM gene was also detected only in a subset of CC224 strains from South Korea. These findings will provide an essential basis for assessing the characteristics of CC224 strains in South Korea that have shown a potential to cause listeriosis outbreaks.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1182090/fullListeria monocytogenesfoodborne outbreakSouth KoreaWGSMLSTllsX |
| spellingShingle | Seung Hun Lee Sangmi Lee Sang Hun Park Ok Kyung Koo Whole-genome sequencing of Listeria monocytogenes isolated from the first listeriosis foodborne outbreak in South Korea Frontiers in Microbiology Listeria monocytogenes foodborne outbreak South Korea WGS MLST llsX |
| title | Whole-genome sequencing of Listeria monocytogenes isolated from the first listeriosis foodborne outbreak in South Korea |
| title_full | Whole-genome sequencing of Listeria monocytogenes isolated from the first listeriosis foodborne outbreak in South Korea |
| title_fullStr | Whole-genome sequencing of Listeria monocytogenes isolated from the first listeriosis foodborne outbreak in South Korea |
| title_full_unstemmed | Whole-genome sequencing of Listeria monocytogenes isolated from the first listeriosis foodborne outbreak in South Korea |
| title_short | Whole-genome sequencing of Listeria monocytogenes isolated from the first listeriosis foodborne outbreak in South Korea |
| title_sort | whole genome sequencing of listeria monocytogenes isolated from the first listeriosis foodborne outbreak in south korea |
| topic | Listeria monocytogenes foodborne outbreak South Korea WGS MLST llsX |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1182090/full |
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