Evaluation of <i>Bacillus velezensis</i> Biocontrol Potential against Fusarium Fungi on Winter Wheat
Fungi of the genus <i>Fusarium</i> are economically significant pathogens in most wheat-growing regions worldwide. The biocontrol agents <i>Bacillus velezensis</i> BZR 336 g and BZR 517 were tested for growth inhibition of <i>F. graminearum</i> BZR 4. The results...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/8/1956 |
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| author | Anzhela Mikhailovna Asaturova Natalya Andreevna Zhevnova Natalia Sergeevna Tomashevich Tatiana Mikhailovna Sidorova Anna Igorevna Homyak Valentina Mikhailovna Dubyaga Vladimir Dmitrievich Nadykta Artem Pavlovich Zharikov Yuri Irodionovich Kostyukevich Boris Sergeevich Tupertsev |
| author_facet | Anzhela Mikhailovna Asaturova Natalya Andreevna Zhevnova Natalia Sergeevna Tomashevich Tatiana Mikhailovna Sidorova Anna Igorevna Homyak Valentina Mikhailovna Dubyaga Vladimir Dmitrievich Nadykta Artem Pavlovich Zharikov Yuri Irodionovich Kostyukevich Boris Sergeevich Tupertsev |
| author_sort | Anzhela Mikhailovna Asaturova |
| collection | DOAJ |
| description | Fungi of the genus <i>Fusarium</i> are economically significant pathogens in most wheat-growing regions worldwide. The biocontrol agents <i>Bacillus velezensis</i> BZR 336 g and BZR 517 were tested for growth inhibition of <i>F. graminearum</i> BZR 4. The results demonstrated that the strains are capable of deforming and destroying hyphae. The modified bioautography technique showed that the strains produce iturin A and surfactin, which probably explains the mechanism of pathogen inhibition. Furthermore, lipopeptides were detected and identified in two samples by the HPLC-HRMS. Compounds such as surfactin and their isomers and homologues were found in both samples. An experiment on an artificial infectious background in a climatic chamber established that the biological effectiveness of strains is close to that of chemical and biological references. Cultivation of plants with <i>B. velezensis</i> showed that the strains are likely to reduce the stress load. An efficacy of up to 45.0% was determined for bioagents BZR 336 g and BZR 517 in field trials, while the yield was up to 7.9 t/ha. The use of <i>B. velezensis</i> BZR 336 g and BZR 517 as biocontrol agents provides an environmentally friendly approach to the control of <i>Fusarium</i> rots on wheat, reduction of the pesticide load, and hence quality harvest. |
| first_indexed | 2024-03-09T10:02:09Z |
| format | Article |
| id | doaj.art-150eab21e4c04ff49738399b0bce6d66 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T10:02:09Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-150eab21e4c04ff49738399b0bce6d662023-12-01T23:18:38ZengMDPI AGAgronomy2073-43952022-08-01128195610.3390/agronomy12081956Evaluation of <i>Bacillus velezensis</i> Biocontrol Potential against Fusarium Fungi on Winter WheatAnzhela Mikhailovna Asaturova0Natalya Andreevna Zhevnova1Natalia Sergeevna Tomashevich2Tatiana Mikhailovna Sidorova3Anna Igorevna Homyak4Valentina Mikhailovna Dubyaga5Vladimir Dmitrievich Nadykta6Artem Pavlovich Zharikov7Yuri Irodionovich Kostyukevich8Boris Sergeevich Tupertsev9Federal Research Center of Biological Plant Protection, 350039 Krasnodar, RussiaFederal Research Center of Biological Plant Protection, 350039 Krasnodar, RussiaFederal Research Center of Biological Plant Protection, 350039 Krasnodar, RussiaFederal Research Center of Biological Plant Protection, 350039 Krasnodar, RussiaFederal Research Center of Biological Plant Protection, 350039 Krasnodar, RussiaFederal Research Center of Biological Plant Protection, 350039 Krasnodar, RussiaFederal Research Center of Biological Plant Protection, 350039 Krasnodar, RussiaSkolkovo Institute of Science and Technology, 121205 Moscow, RussiaSkolkovo Institute of Science and Technology, 121205 Moscow, RussiaSkolkovo Institute of Science and Technology, 121205 Moscow, RussiaFungi of the genus <i>Fusarium</i> are economically significant pathogens in most wheat-growing regions worldwide. The biocontrol agents <i>Bacillus velezensis</i> BZR 336 g and BZR 517 were tested for growth inhibition of <i>F. graminearum</i> BZR 4. The results demonstrated that the strains are capable of deforming and destroying hyphae. The modified bioautography technique showed that the strains produce iturin A and surfactin, which probably explains the mechanism of pathogen inhibition. Furthermore, lipopeptides were detected and identified in two samples by the HPLC-HRMS. Compounds such as surfactin and their isomers and homologues were found in both samples. An experiment on an artificial infectious background in a climatic chamber established that the biological effectiveness of strains is close to that of chemical and biological references. Cultivation of plants with <i>B. velezensis</i> showed that the strains are likely to reduce the stress load. An efficacy of up to 45.0% was determined for bioagents BZR 336 g and BZR 517 in field trials, while the yield was up to 7.9 t/ha. The use of <i>B. velezensis</i> BZR 336 g and BZR 517 as biocontrol agents provides an environmentally friendly approach to the control of <i>Fusarium</i> rots on wheat, reduction of the pesticide load, and hence quality harvest.https://www.mdpi.com/2073-4395/12/8/1956<i>Bacillus velezensis</i><i>Fusarium graminearum</i>biocontrolwinter wheat |
| spellingShingle | Anzhela Mikhailovna Asaturova Natalya Andreevna Zhevnova Natalia Sergeevna Tomashevich Tatiana Mikhailovna Sidorova Anna Igorevna Homyak Valentina Mikhailovna Dubyaga Vladimir Dmitrievich Nadykta Artem Pavlovich Zharikov Yuri Irodionovich Kostyukevich Boris Sergeevich Tupertsev Evaluation of <i>Bacillus velezensis</i> Biocontrol Potential against Fusarium Fungi on Winter Wheat Agronomy <i>Bacillus velezensis</i> <i>Fusarium graminearum</i> biocontrol winter wheat |
| title | Evaluation of <i>Bacillus velezensis</i> Biocontrol Potential against Fusarium Fungi on Winter Wheat |
| title_full | Evaluation of <i>Bacillus velezensis</i> Biocontrol Potential against Fusarium Fungi on Winter Wheat |
| title_fullStr | Evaluation of <i>Bacillus velezensis</i> Biocontrol Potential against Fusarium Fungi on Winter Wheat |
| title_full_unstemmed | Evaluation of <i>Bacillus velezensis</i> Biocontrol Potential against Fusarium Fungi on Winter Wheat |
| title_short | Evaluation of <i>Bacillus velezensis</i> Biocontrol Potential against Fusarium Fungi on Winter Wheat |
| title_sort | evaluation of i bacillus velezensis i biocontrol potential against fusarium fungi on winter wheat |
| topic | <i>Bacillus velezensis</i> <i>Fusarium graminearum</i> biocontrol winter wheat |
| url | https://www.mdpi.com/2073-4395/12/8/1956 |
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