Txnip inhibits porcine adipocyte differentiation through PPARγ and impairs the induction of glucose via ChREBP
ABSTRACT To explore the functions of Txnip and its mechanism in adipocyte differentiation, the preadipocytes were isolated from subcutaneous adipose tissue of three-day-old piglets and induced into adipogenic differentiation. The expression of Txnip and ChREBP was silenced, and the Txnip overexpress...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Zootecnia
2023-11-01
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| Series: | Revista Brasileira de Zootecnia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982023000100405&tlng=en |
| _version_ | 1797449397416493056 |
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| author | Xiao Ning Dou Fang Wei Jing Zhang Cheng Cheng Tian Lu Fu Su Su Jiang Jian Xiong Lu Guo Hua Zhang |
| author_facet | Xiao Ning Dou Fang Wei Jing Zhang Cheng Cheng Tian Lu Fu Su Su Jiang Jian Xiong Lu Guo Hua Zhang |
| author_sort | Xiao Ning Dou |
| collection | DOAJ |
| description | ABSTRACT To explore the functions of Txnip and its mechanism in adipocyte differentiation, the preadipocytes were isolated from subcutaneous adipose tissue of three-day-old piglets and induced into adipogenic differentiation. The expression of Txnip and ChREBP was silenced, and the Txnip overexpression was achieved in the cells with transfection of the recombinant lentivirus strategies. Txnip silencing promoted the differentiation of porcine preadipocytes and PPARγ expression, and a PPARγ inhibitor reduced this facilitation. Instead, Txnip overexpression exerted a suppressive effect on the cell differentiation and PPARγ expression, and the PPARγ agonist offset this inhibition. High glucose stimulated the preadipocyte differentiation and expressions of ChREBP and Txnip. In contrast, Txnip expression was reduced by ChREBP silencing, suggesting glucose-regulated Txnip expression through the mediation of ChREBP. Moreover, the expressions of ChREBP and Glut4 induced by high glucose and glucose uptake of the cells were reduced by Txnip-overexpression, but increased by Txnip silencing, while these changes of Txnip did not alter their expressions under low glucose. Collectively, Txnip could be an inhibitor of porcine preadipocyte differentiation, which attenuated the adipogenesis through the negative feedback regulation on PPARγ. Txnip impaired the induction of glucose on the preadipocyte differentiation through decreasing Glut4 expression and glucose uptake and subsequent decrease of the expression and transcriptional activity of ChREBP. |
| first_indexed | 2024-03-09T14:24:29Z |
| format | Article |
| id | doaj.art-1510cec337df49689fbb9b0acbd8fc3b |
| institution | Directory Open Access Journal |
| issn | 1806-9290 |
| language | English |
| last_indexed | 2024-03-09T14:24:29Z |
| publishDate | 2023-11-01 |
| publisher | Sociedade Brasileira de Zootecnia |
| record_format | Article |
| series | Revista Brasileira de Zootecnia |
| spelling | doaj.art-1510cec337df49689fbb9b0acbd8fc3b2023-11-28T07:43:25ZengSociedade Brasileira de ZootecniaRevista Brasileira de Zootecnia1806-92902023-11-015210.37496/rbz5220220029Txnip inhibits porcine adipocyte differentiation through PPARγ and impairs the induction of glucose via ChREBPXiao Ning Douhttps://orcid.org/0000-0002-8382-3365Fang Weihttps://orcid.org/0000-0001-5098-0551Jing Zhanghttps://orcid.org/0000-0002-5164-2180Cheng Cheng Tianhttps://orcid.org/0000-0003-1954-0149Lu Fuhttps://orcid.org/0000-0003-3953-2621Su Su Jianghttps://orcid.org/0000-0002-8213-6264Jian Xiong Luhttps://orcid.org/0000-0003-4399-1572Guo Hua Zhanghttps://orcid.org/0000-0001-9558-1044ABSTRACT To explore the functions of Txnip and its mechanism in adipocyte differentiation, the preadipocytes were isolated from subcutaneous adipose tissue of three-day-old piglets and induced into adipogenic differentiation. The expression of Txnip and ChREBP was silenced, and the Txnip overexpression was achieved in the cells with transfection of the recombinant lentivirus strategies. Txnip silencing promoted the differentiation of porcine preadipocytes and PPARγ expression, and a PPARγ inhibitor reduced this facilitation. Instead, Txnip overexpression exerted a suppressive effect on the cell differentiation and PPARγ expression, and the PPARγ agonist offset this inhibition. High glucose stimulated the preadipocyte differentiation and expressions of ChREBP and Txnip. In contrast, Txnip expression was reduced by ChREBP silencing, suggesting glucose-regulated Txnip expression through the mediation of ChREBP. Moreover, the expressions of ChREBP and Glut4 induced by high glucose and glucose uptake of the cells were reduced by Txnip-overexpression, but increased by Txnip silencing, while these changes of Txnip did not alter their expressions under low glucose. Collectively, Txnip could be an inhibitor of porcine preadipocyte differentiation, which attenuated the adipogenesis through the negative feedback regulation on PPARγ. Txnip impaired the induction of glucose on the preadipocyte differentiation through decreasing Glut4 expression and glucose uptake and subsequent decrease of the expression and transcriptional activity of ChREBP.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982023000100405&tlng=enadipose tissuelipidpig |
| spellingShingle | Xiao Ning Dou Fang Wei Jing Zhang Cheng Cheng Tian Lu Fu Su Su Jiang Jian Xiong Lu Guo Hua Zhang Txnip inhibits porcine adipocyte differentiation through PPARγ and impairs the induction of glucose via ChREBP Revista Brasileira de Zootecnia adipose tissue lipid pig |
| title | Txnip inhibits porcine adipocyte differentiation through PPARγ and impairs the induction of glucose via ChREBP |
| title_full | Txnip inhibits porcine adipocyte differentiation through PPARγ and impairs the induction of glucose via ChREBP |
| title_fullStr | Txnip inhibits porcine adipocyte differentiation through PPARγ and impairs the induction of glucose via ChREBP |
| title_full_unstemmed | Txnip inhibits porcine adipocyte differentiation through PPARγ and impairs the induction of glucose via ChREBP |
| title_short | Txnip inhibits porcine adipocyte differentiation through PPARγ and impairs the induction of glucose via ChREBP |
| title_sort | txnip inhibits porcine adipocyte differentiation through pparγ and impairs the induction of glucose via chrebp |
| topic | adipose tissue lipid pig |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982023000100405&tlng=en |
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