A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3
Recently, the highly pathogenic serotype 4 fowl adenovirus (FAdV-4) and duck adenovirus 3 (DAdV-3) were outbroken and widespread, causing substantial economic losses to the duck industry. Therefore, there is an urgent need to generate a recombinant genetic engineering vaccine candidate against both...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1177866/full |
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| author | Yiwen Guo Yiwen Guo Yiwen Guo Yiwen Guo Yun Lin Yun Lin Yun Lin Quan Xie Quan Xie Quan Xie Quan Xie Wenyuan Zhang Wenyuan Zhang Wenyuan Zhang Wenyuan Zhang Zhenqi Xu Zhenqi Xu Zhenqi Xu Zhenqi Xu Yifei Chao Yifei Chao Yifei Chao Yifei Chao Xudong Cao Xudong Cao Xudong Cao Xudong Cao Huiru Jiang Huiru Jiang Huiru Jiang Huiru Jiang Han Li Han Li Han Li Han Li Tuofan Li Tuofan Li Tuofan Li Tuofan Li Zhimin Wan Zhimin Wan Zhimin Wan Zhimin Wan Hongxia Shao Hongxia Shao Hongxia Shao Hongxia Shao Aijian Qin Aijian Qin Aijian Qin Aijian Qin Jianqiang Ye Jianqiang Ye Jianqiang Ye Jianqiang Ye |
| author_facet | Yiwen Guo Yiwen Guo Yiwen Guo Yiwen Guo Yun Lin Yun Lin Yun Lin Quan Xie Quan Xie Quan Xie Quan Xie Wenyuan Zhang Wenyuan Zhang Wenyuan Zhang Wenyuan Zhang Zhenqi Xu Zhenqi Xu Zhenqi Xu Zhenqi Xu Yifei Chao Yifei Chao Yifei Chao Yifei Chao Xudong Cao Xudong Cao Xudong Cao Xudong Cao Huiru Jiang Huiru Jiang Huiru Jiang Huiru Jiang Han Li Han Li Han Li Han Li Tuofan Li Tuofan Li Tuofan Li Tuofan Li Zhimin Wan Zhimin Wan Zhimin Wan Zhimin Wan Hongxia Shao Hongxia Shao Hongxia Shao Hongxia Shao Aijian Qin Aijian Qin Aijian Qin Aijian Qin Jianqiang Ye Jianqiang Ye Jianqiang Ye Jianqiang Ye |
| author_sort | Yiwen Guo |
| collection | DOAJ |
| description | Recently, the highly pathogenic serotype 4 fowl adenovirus (FAdV-4) and duck adenovirus 3 (DAdV-3) were outbroken and widespread, causing substantial economic losses to the duck industry. Therefore, there is an urgent need to generate a recombinant genetic engineering vaccine candidate against both FAdV-4 and DAdV-3. In this study, a novel recombinant FAdV-4 expressing the Fiber-2 protein of DAdV-3, designated as rFAdV-4-Fiber-2/DAdV-3, was generated based on CRISPR/Cas9 and Cre-LoxP systems. Indirect immunofluorescence assay (IFA) and western blot (WB) showed that the Fiber-2 protein of DAdV-3 in rFAdV-4-Fiber-2/DAdV-3 was expressed successfully. Moreover, the growth curve revealed that rFAdV-4-Fiber-2/DAdV-3 replicated efficiently in LMH cells and even showed a stronger replication ability compared to the wild type FAdV-4. The generation of the recombinant rFAdV-4-Fiber-2/DAdV-3 provides a potential vaccine candidate against both FAdV-4 and DAdV-3. |
| first_indexed | 2024-04-09T21:02:38Z |
| format | Article |
| id | doaj.art-1516da5d5b7f42938decf5a46bb1e595 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-09T21:02:38Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-1516da5d5b7f42938decf5a46bb1e5952023-03-29T06:14:42ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-03-011310.3389/fcimb.2023.11778661177866A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3Yiwen Guo0Yiwen Guo1Yiwen Guo2Yiwen Guo3Yun Lin4Yun Lin5Yun Lin6Quan Xie7Quan Xie8Quan Xie9Quan Xie10Wenyuan Zhang11Wenyuan Zhang12Wenyuan Zhang13Wenyuan Zhang14Zhenqi Xu15Zhenqi Xu16Zhenqi Xu17Zhenqi Xu18Yifei Chao19Yifei Chao20Yifei Chao21Yifei Chao22Xudong Cao23Xudong Cao24Xudong Cao25Xudong Cao26Huiru Jiang27Huiru Jiang28Huiru Jiang29Huiru Jiang30Han Li31Han Li32Han Li33Han Li34Tuofan Li35Tuofan Li36Tuofan Li37Tuofan Li38Zhimin Wan39Zhimin Wan40Zhimin Wan41Zhimin Wan42Hongxia Shao43Hongxia Shao44Hongxia Shao45Hongxia Shao46Aijian Qin47Aijian Qin48Aijian Qin49Aijian Qin50Jianqiang Ye51Jianqiang Ye52Jianqiang Ye53Jianqiang Ye54Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, ChinaInstitutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, ChinaRecently, the highly pathogenic serotype 4 fowl adenovirus (FAdV-4) and duck adenovirus 3 (DAdV-3) were outbroken and widespread, causing substantial economic losses to the duck industry. Therefore, there is an urgent need to generate a recombinant genetic engineering vaccine candidate against both FAdV-4 and DAdV-3. In this study, a novel recombinant FAdV-4 expressing the Fiber-2 protein of DAdV-3, designated as rFAdV-4-Fiber-2/DAdV-3, was generated based on CRISPR/Cas9 and Cre-LoxP systems. Indirect immunofluorescence assay (IFA) and western blot (WB) showed that the Fiber-2 protein of DAdV-3 in rFAdV-4-Fiber-2/DAdV-3 was expressed successfully. Moreover, the growth curve revealed that rFAdV-4-Fiber-2/DAdV-3 replicated efficiently in LMH cells and even showed a stronger replication ability compared to the wild type FAdV-4. The generation of the recombinant rFAdV-4-Fiber-2/DAdV-3 provides a potential vaccine candidate against both FAdV-4 and DAdV-3.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1177866/fullDAdV-3fiber-2recombinant FAdV-4CRISPR/Cas9expressionreplication |
| spellingShingle | Yiwen Guo Yiwen Guo Yiwen Guo Yiwen Guo Yun Lin Yun Lin Yun Lin Quan Xie Quan Xie Quan Xie Quan Xie Wenyuan Zhang Wenyuan Zhang Wenyuan Zhang Wenyuan Zhang Zhenqi Xu Zhenqi Xu Zhenqi Xu Zhenqi Xu Yifei Chao Yifei Chao Yifei Chao Yifei Chao Xudong Cao Xudong Cao Xudong Cao Xudong Cao Huiru Jiang Huiru Jiang Huiru Jiang Huiru Jiang Han Li Han Li Han Li Han Li Tuofan Li Tuofan Li Tuofan Li Tuofan Li Zhimin Wan Zhimin Wan Zhimin Wan Zhimin Wan Hongxia Shao Hongxia Shao Hongxia Shao Hongxia Shao Aijian Qin Aijian Qin Aijian Qin Aijian Qin Jianqiang Ye Jianqiang Ye Jianqiang Ye Jianqiang Ye A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3 Frontiers in Cellular and Infection Microbiology DAdV-3 fiber-2 recombinant FAdV-4 CRISPR/Cas9 expression replication |
| title | A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3 |
| title_full | A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3 |
| title_fullStr | A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3 |
| title_full_unstemmed | A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3 |
| title_short | A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3 |
| title_sort | novel recombinant serotype 4 fowl adenovirus expressing fiber 2 protein of duck adenovirus 3 |
| topic | DAdV-3 fiber-2 recombinant FAdV-4 CRISPR/Cas9 expression replication |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1177866/full |
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