Self-Assembled MoS<sub>2</sub>/ssDNA Nanostructures for the Capacitive Aptasensing of Acetamiprid Insecticide
The aim of this work is to detect acetamiprid using electrochemical capacitance spectroscopy, which is widely used as a pesticide in agriculture and is harmful to humans. We have designed aptasensing platform based on the adsorption of a DNA aptamer on lipoic acid-modified MoS<sub>2</sub>...
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MDPI AG
2021-02-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/4/1382 |
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| author | Maroua Hamami Noureddine Raouafi Hafsa Korri-Youssoufi |
| author_facet | Maroua Hamami Noureddine Raouafi Hafsa Korri-Youssoufi |
| author_sort | Maroua Hamami |
| collection | DOAJ |
| description | The aim of this work is to detect acetamiprid using electrochemical capacitance spectroscopy, which is widely used as a pesticide in agriculture and is harmful to humans. We have designed aptasensing platform based on the adsorption of a DNA aptamer on lipoic acid-modified MoS<sub>2</sub> nano-sheets. The biosensor takes advantage of the high affinity of single-stranded DNA sequences to MoS<sub>2</sub> nano-sheets. The stability of DNA on MoS<sub>2</sub> nano-sheets is assured by covalent attachment to lipoic acid that forms self-assembled layer on MoS<sub>2</sub> surface. The biosensor exhibits excellent capacitance performances owing to its large effective surface area making it interesting material for capacitive transduction system. The impedance-derived capacitance varies with the increasing concentrations of acetamiprid that can be attributed to the aptamer desorption from the MoS<sub>2</sub> nanosheets facilitating ion diffusion into MoS<sub>2</sub> interlayers. The developed device showed high analytical performances for acetamiprid detection on electrochemical impedance spectroscopy EIS- derived capacitance variation and high selectivity toward the target in presence of other pesticides. Real sample analysis of food stuff such as tomatoes is demonstrated which open the way to their use for monitoring of food contaminants by tailoring the aptamer. |
| first_indexed | 2024-03-09T05:52:53Z |
| format | Article |
| id | doaj.art-1518fdf9008b4325af3af2e2b3948902 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T05:52:53Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-1518fdf9008b4325af3af2e2b39489022023-12-03T12:15:43ZengMDPI AGApplied Sciences2076-34172021-02-01114138210.3390/app11041382Self-Assembled MoS<sub>2</sub>/ssDNA Nanostructures for the Capacitive Aptasensing of Acetamiprid InsecticideMaroua Hamami0Noureddine Raouafi1Hafsa Korri-Youssoufi2Université Paris-Saclay, CNRS, Institut de Chimie Moléculaire et des Matériaux d’Orsay (ICMMO), ECBB, Bât 420, 2 Rue du Doyen Georges Poitou, 91400 Orsay, FranceFaculté des Sciences de Tunis El Manar, Laboratoire de Chimie Analytique et Electrochimie (LR99ES15), Campus Universitaire de Tunis El Manar, Université de Tunis El Manar, Tunis El–Manar 2092, TunisiaFaculté des Sciences de Tunis El Manar, Laboratoire de Chimie Analytique et Electrochimie (LR99ES15), Campus Universitaire de Tunis El Manar, Université de Tunis El Manar, Tunis El–Manar 2092, TunisiaThe aim of this work is to detect acetamiprid using electrochemical capacitance spectroscopy, which is widely used as a pesticide in agriculture and is harmful to humans. We have designed aptasensing platform based on the adsorption of a DNA aptamer on lipoic acid-modified MoS<sub>2</sub> nano-sheets. The biosensor takes advantage of the high affinity of single-stranded DNA sequences to MoS<sub>2</sub> nano-sheets. The stability of DNA on MoS<sub>2</sub> nano-sheets is assured by covalent attachment to lipoic acid that forms self-assembled layer on MoS<sub>2</sub> surface. The biosensor exhibits excellent capacitance performances owing to its large effective surface area making it interesting material for capacitive transduction system. The impedance-derived capacitance varies with the increasing concentrations of acetamiprid that can be attributed to the aptamer desorption from the MoS<sub>2</sub> nanosheets facilitating ion diffusion into MoS<sub>2</sub> interlayers. The developed device showed high analytical performances for acetamiprid detection on electrochemical impedance spectroscopy EIS- derived capacitance variation and high selectivity toward the target in presence of other pesticides. Real sample analysis of food stuff such as tomatoes is demonstrated which open the way to their use for monitoring of food contaminants by tailoring the aptamer.https://www.mdpi.com/2076-3417/11/4/1382aptasensorMoS<sub>2</sub>pesticideneonicotinoidcapacitance |
| spellingShingle | Maroua Hamami Noureddine Raouafi Hafsa Korri-Youssoufi Self-Assembled MoS<sub>2</sub>/ssDNA Nanostructures for the Capacitive Aptasensing of Acetamiprid Insecticide Applied Sciences aptasensor MoS<sub>2</sub> pesticide neonicotinoid capacitance |
| title | Self-Assembled MoS<sub>2</sub>/ssDNA Nanostructures for the Capacitive Aptasensing of Acetamiprid Insecticide |
| title_full | Self-Assembled MoS<sub>2</sub>/ssDNA Nanostructures for the Capacitive Aptasensing of Acetamiprid Insecticide |
| title_fullStr | Self-Assembled MoS<sub>2</sub>/ssDNA Nanostructures for the Capacitive Aptasensing of Acetamiprid Insecticide |
| title_full_unstemmed | Self-Assembled MoS<sub>2</sub>/ssDNA Nanostructures for the Capacitive Aptasensing of Acetamiprid Insecticide |
| title_short | Self-Assembled MoS<sub>2</sub>/ssDNA Nanostructures for the Capacitive Aptasensing of Acetamiprid Insecticide |
| title_sort | self assembled mos sub 2 sub ssdna nanostructures for the capacitive aptasensing of acetamiprid insecticide |
| topic | aptasensor MoS<sub>2</sub> pesticide neonicotinoid capacitance |
| url | https://www.mdpi.com/2076-3417/11/4/1382 |
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