Antibacterial Activity of PVA Hydrogels Embedding Oxide Nanostructures Sensitized by Noble Metals and Ruthenium Dye
Nanostructured oxides (SiO<sub>2</sub>, TiO<sub>2</sub>) were synthesized using the sol–gel method and modified with noble metal nanoparticles (Pt, Au) and ruthenium dye to enhance light harvesting and promote the photogeneration of reactive oxygen species, namely singlet oxy...
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MDPI AG
2023-08-01
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| Online Access: | https://www.mdpi.com/2310-2861/9/8/650 |
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| author | Diana Pelinescu Mihai Anastasescu Veronica Bratan Valentin-Adrian Maraloiu Catalin Negrila Daiana Mitrea Jose Calderon-Moreno Silviu Preda Ioana Catalina Gîfu Adrian Stan Robertina Ionescu Ileana Stoica Crina Anastasescu Maria Zaharescu Ioan Balint |
| author_facet | Diana Pelinescu Mihai Anastasescu Veronica Bratan Valentin-Adrian Maraloiu Catalin Negrila Daiana Mitrea Jose Calderon-Moreno Silviu Preda Ioana Catalina Gîfu Adrian Stan Robertina Ionescu Ileana Stoica Crina Anastasescu Maria Zaharescu Ioan Balint |
| author_sort | Diana Pelinescu |
| collection | DOAJ |
| description | Nanostructured oxides (SiO<sub>2</sub>, TiO<sub>2</sub>) were synthesized using the sol–gel method and modified with noble metal nanoparticles (Pt, Au) and ruthenium dye to enhance light harvesting and promote the photogeneration of reactive oxygen species, namely singlet oxygen (<sup>1</sup>O<sub>2</sub>) and hydroxyl radical (•OH). The resulting nanostructures were embedded in a transparent polyvinyl alcohol (PVA) hydrogel. Morphological and structural characterization of the bare and modified oxides was performed using scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM), UV–Vis spectroscopy, and X-ray photoelectron spectroscopy (XPS). Additionally, electrokinetic potential measurements were conducted. Crystallinity data and elemental analysis of the investigated systems were obtained through X-ray diffraction and X-ray fluorescence analyses, while the chemical state of the elements was determined using XPS. The engineered materials, both as simple powders and embedded in the hydrogel, were evaluated for their ability to generate reactive oxygen species (ROS) under visible and simulated solar light irradiation to establish a correlation with their antibacterial activity against <i>Staphylococcus aureus</i>. The generation of singlet oxygen (<sup>1</sup>O<sub>2</sub>) by the samples under visible light exposure can be of significant importance for their potential use in biomedical applications. |
| first_indexed | 2024-03-10T23:55:00Z |
| format | Article |
| id | doaj.art-1519a812c0d3430dbcdde5fa593c4f16 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-10T23:55:00Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-1519a812c0d3430dbcdde5fa593c4f162023-11-19T01:13:50ZengMDPI AGGels2310-28612023-08-019865010.3390/gels9080650Antibacterial Activity of PVA Hydrogels Embedding Oxide Nanostructures Sensitized by Noble Metals and Ruthenium DyeDiana Pelinescu0Mihai Anastasescu1Veronica Bratan2Valentin-Adrian Maraloiu3Catalin Negrila4Daiana Mitrea5Jose Calderon-Moreno6Silviu Preda7Ioana Catalina Gîfu8Adrian Stan9Robertina Ionescu10Ileana Stoica11Crina Anastasescu12Maria Zaharescu13Ioan Balint14Faculty of Biology, Intrarea Portocalilor 1–3, Sector 5, 060101 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, RomaniaNational Institute of Materials Physics, 405A Atomistilor St., 077125 Magurele, Ilfov, RomaniaNational Institute of Materials Physics, 405A Atomistilor St., 077125 Magurele, Ilfov, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, RomaniaNational Institute for Research and Development in Chemistry and Petrochemistry-ICECHIM, 202 Spl. Independentei, 060021 Bucharest, RomaniaTechir Cosmetics SRL, Plantelor Str., 907015 Agigea, RomaniaFaculty of Biology, Intrarea Portocalilor 1–3, Sector 5, 060101 Bucharest, RomaniaFaculty of Biology, Intrarea Portocalilor 1–3, Sector 5, 060101 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, RomaniaNanostructured oxides (SiO<sub>2</sub>, TiO<sub>2</sub>) were synthesized using the sol–gel method and modified with noble metal nanoparticles (Pt, Au) and ruthenium dye to enhance light harvesting and promote the photogeneration of reactive oxygen species, namely singlet oxygen (<sup>1</sup>O<sub>2</sub>) and hydroxyl radical (•OH). The resulting nanostructures were embedded in a transparent polyvinyl alcohol (PVA) hydrogel. Morphological and structural characterization of the bare and modified oxides was performed using scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM), UV–Vis spectroscopy, and X-ray photoelectron spectroscopy (XPS). Additionally, electrokinetic potential measurements were conducted. Crystallinity data and elemental analysis of the investigated systems were obtained through X-ray diffraction and X-ray fluorescence analyses, while the chemical state of the elements was determined using XPS. The engineered materials, both as simple powders and embedded in the hydrogel, were evaluated for their ability to generate reactive oxygen species (ROS) under visible and simulated solar light irradiation to establish a correlation with their antibacterial activity against <i>Staphylococcus aureus</i>. The generation of singlet oxygen (<sup>1</sup>O<sub>2</sub>) by the samples under visible light exposure can be of significant importance for their potential use in biomedical applications.https://www.mdpi.com/2310-2861/9/8/650optic active SiO<sub>2</sub>TiO<sub>2</sub>noble metal nanoparticlessensitizersinglet oxygenhydroxyl radical |
| spellingShingle | Diana Pelinescu Mihai Anastasescu Veronica Bratan Valentin-Adrian Maraloiu Catalin Negrila Daiana Mitrea Jose Calderon-Moreno Silviu Preda Ioana Catalina Gîfu Adrian Stan Robertina Ionescu Ileana Stoica Crina Anastasescu Maria Zaharescu Ioan Balint Antibacterial Activity of PVA Hydrogels Embedding Oxide Nanostructures Sensitized by Noble Metals and Ruthenium Dye Gels optic active SiO<sub>2</sub> TiO<sub>2</sub> noble metal nanoparticles sensitizer singlet oxygen hydroxyl radical |
| title | Antibacterial Activity of PVA Hydrogels Embedding Oxide Nanostructures Sensitized by Noble Metals and Ruthenium Dye |
| title_full | Antibacterial Activity of PVA Hydrogels Embedding Oxide Nanostructures Sensitized by Noble Metals and Ruthenium Dye |
| title_fullStr | Antibacterial Activity of PVA Hydrogels Embedding Oxide Nanostructures Sensitized by Noble Metals and Ruthenium Dye |
| title_full_unstemmed | Antibacterial Activity of PVA Hydrogels Embedding Oxide Nanostructures Sensitized by Noble Metals and Ruthenium Dye |
| title_short | Antibacterial Activity of PVA Hydrogels Embedding Oxide Nanostructures Sensitized by Noble Metals and Ruthenium Dye |
| title_sort | antibacterial activity of pva hydrogels embedding oxide nanostructures sensitized by noble metals and ruthenium dye |
| topic | optic active SiO<sub>2</sub> TiO<sub>2</sub> noble metal nanoparticles sensitizer singlet oxygen hydroxyl radical |
| url | https://www.mdpi.com/2310-2861/9/8/650 |
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